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+ < meta http-equiv ="Content-type " content ="text/html;charset=utf-8 " />
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+ < link rel ="stylesheet " type ="text/css " href ="./index.css " />
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+ < title > NGS分析</ title >
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+ </ head >
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+ < body >
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+ < center > < h1 > NGS分析</ h1 > </ center >
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+ < hr />
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+ < p class ="version "> 该实验指导(word)依旧延续第一版风格——以The Pennsylvania State University 所开设的< a href ="http://www.personal.psu.edu/iua1/courses/2012-BMMB-597D.html "> 《Analyzing High Throughput Sequencing Data》</ a > 为主线,讲解了NGS分析的入门知识。与第一版相比,第二版更加详细的介绍了生物信息学分析中常用的格式,同时增加了更过网络文本、视频资料,以便更好的掌握知识。</ p >
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+ < p > 实验指导(word)在此处下载:< a href ="./doDownload.php?tag=00:NGS分析篇.pdf "> PDF版</ a >   < a href ="./doDownload.php?tag=00:NGS分析篇.docx "> WORD版</ a > </ p >
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+
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+ < table >
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+ < tr >
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+ < td class ="title "> 实验7 Linux系统中进行GFF文件处理入门</ td >
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+ < td class ="context ">
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+ < ul >
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+ < li > 从< a href ="http://www.yeastgenome.org/ "> 酵母菌基因组数据库(SGD)</ a > 下载酵母菌基因组注释信息< a href ="./doDownload.php?tag=sc.gff "> sc.gff</ a > </ li >
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+ < li > 掌握< a href ="https://github.com/The-Sequence-Ontology/Specifications/blob/master/gff3.md "> GFF3格式</ a > </ li >
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+ < li > 使用Linux常用的文本处理命令:< code > wc</ code > < code > grep</ code > < code > cut</ code > < code > head</ code > 等处理GFF文件</ li >
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+ < li > 相关下载:
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+ < a href ="./doDownload.php?tag=01:实验7_Linux系统中进行GFF文件处理入门.docx "> 实验指导(实验7/word)</ a >
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+ < a href ="./doDownload.php?tag=01:putty.exe "> putty.exe</ a >
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+ </ li >
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+ </ ul >
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+ </ td >
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+ < tr >
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+ < tr >
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+ < td class ="title "> 实验8 NGS数据初步分析</ td >
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+ < td class ="context ">
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+ < ul >
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+ < li > < a href ="http://blog.csdn.net/dyllove98/article/details/9736441 "> 三代测序的原理</ a > </ li >
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+ < li > < a href ="http://v.youku.com/v_show/id_XNzk3NTI0Mjc2.html "> Illumina测序化学原理</ a > </ li >
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+ < li > 介绍测序数据的质量控制过程</ li >
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+ < li > 相关下载:
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+ < a href ="./doDownload.php?tag=01:实验8_NGS数据初步分析.docx "> 实验指导(实验8/word)</ a >
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+ < a href ="./doDownload.php?tag=02:sample1.fq "> 样本数据sample1.fq</ a >
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+ < a href ="./doDownload.php?tag=02:WinSCP-5.9.3-Setup.exe "> WinSCP</ a >
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+ < a href ="./doDownload.php?tag=02:fastqc_v0.11.5.zip "> fastqc</ a >
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+ < a href ="./doDownload.php?tag=02:fastx_toolkit_0.0.13_binaries_Linux_2.6_amd64.tar.bz2 "> fastx_toolkit</ a >
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+ </ li >
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+ </ ul >
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+ </ td >
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+ < tr >
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+ < tr >
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+ < td class ="title "> 实验9 短序列比对</ td >
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+ < td class ="context ">
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+ < ul >
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+ < li > 介绍短序列比对的方法</ li >
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+ < li > SAM格式</ li >
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+ < li > 相关下载:
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+ < a href ="./doDownload.php?tag=03:实验9_短序列组装.docx "> 实验指导(实验9/word)</ a >
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+ < a href ="./doDownload.php?tag=03:test.fq "> 测试数据test.fq</ a >
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+ < a href ="./doDownload.php?tag=03:SAM详解.pdf "> SAM格式说明</ a >
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+ < a href ="./doDownload.php?tag=03:bwa-0.7.15.tar.bz2 "> bwa</ a >
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+ < a href ="./doDownload.php?tag=03:samtools-1.3.1.tar.bz2 "> samtools</ a >
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+ </ li >
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+ </ ul >
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+ </ td >
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+ </ tr >
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+ < tr >
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+ < td class ="title "> 实验10 基因组的可视化</ td >
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+ < td class ="context ">
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+ < ul >
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+ < li > IGV的使用</ li >
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+ < li > 相关下载:
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+ < a href ="./doDownload.php?tag=04:实验10_基因组的可视化.docx "> 实验指导(实验10/word)</ a >
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+ < a href ="./doDownload.php?tag=04:IGV_2.3.90.rar "> IGV</ a >
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+ </ li >
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+ </ ul >
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+ </ td >
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+ </ tr >
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+ < tr >
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+ < td class ="title "> 实验11 NGS数据初步分析</ td >
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+ < td class ="context ">
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+ < ul >
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+ < li > 测序数据的模拟方法</ li >
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+ < li > SNP calling的方法</ li >
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+ < li > VCF格式</ li >
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+ < li > 相关下载:
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+ < a href ="./doDownload.php?tag=05:实验11_识别SNP.docx "> 实验指导(实验11/word)</ a >
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+ < a href ="./doDownload.php?tag=05:VCFv4.2.pdf "> VCF格式说明</ a >
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+ < a href ="./doDownload.php?tag=05:bcftools-1.3.1.tar.bz2 "> bcftools</ a >
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+ </ li >
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+ </ ul >
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+ </ td >
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+ </ tr >
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+ < tr >
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+ < td class ="title "> 优秀的博客资源</ td >
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+ < td class ="context ">
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+ < ul >
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+ < li > < a href ="http://www.biotrainee.com/index.php "> 生物技能树</ a > </ li >
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+ < li > < a href ="http://biobits.org/samtools_primer.html "> SAMtools Tutorial</ a > (与实验指导相互参照学习,可促进你更好的理解)</ li >
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+ < li > < a href ="http://blog.qiubio.com:8080/rbioconductor-2 "> 糗世界</ a > (R/Bioconductor学习可以参考)</ li >
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+ </ ul >
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+ </ td >
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+ </ tr >
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+ </ table >
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+ </ body >
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+ </ html >
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