La primera cadena de
ADNc fue sintetizada mediante el Sistema de Sintesis RiboClone[R]
ADNc (Promega 2005) con la pareja de cebadores oligo(dT)16 (5'-[AACATATGCGGCCGCATTATGGCCTTT.sub.16]-3' y 5'-GATCTTCCACGCGTCGAC[(T).sub.16] 3') y a partir de las condiciones de amplificacion recomendadas en el prospecto del juego de reactivos Marathon[TM].
Para la construccion del
ADNc, 1 [micron]g del ARNm se puso en contacto con 50 ng/ml de cebadores aleatorios, 0,5 mM de dNTP, 0,005 M de DDT y 200 U de la enzima SuperScript III, en un volumen final de 20 [micron]l (Invitrogen).
Meanwhile, the top volume stock companies were (GPI), (INVESTORS), (
ADNC), (ITHMAR), and (HITSTELEC).
Meanwhile, the top volume stock companies were (
ADNC), (ITHMAR), (ALMOUDON), (AJWAN), and (MAYADEEN).
Meanwhile, the top volume stock companies were (
ADNC), (ITHMR), (MAYADEEN), (INVESTORS), AND (AJWAN).
Meanwhile, the top volume stock companies were (CBK), (
ADNC), (ALMADINA), (INVESTORS), AND (ZIMAH).
(end) Top share gainers companies were (CABLETV), (REAM), (YIACO), (KMEFIC), and (ALSALAM), while top volume stock companies were (ALIMRIAZ), (
ADNC), (SENERGY), (KBT), and (INVESTORS).
The top gainers were (REAM), (UPAC), (QURAINHLD), (ASC) and (MENA), while the top volume shares were (ALIMTIAZ), (
ADNC), (INVESTORS), (GIH) and (ALMUDON).(end) ag
The top gainers were (RKWC), (NINV), (HUMANSOFT), (TAMINV) and (MARIN), while the top volume shares were (
ADNC), (ALMADINA), (COAST), (HITSTELEC) and (GNAHC).(end) fh.tb.ag
The top gainers were (INVESTORS), (KPPC), (HAYATCOMM), (FUTURE) and (MAYADEEN), while the top volume stocks were (
ADNC), (INVESTORS), (GPI), (MAYADEEN) and (ALMUDON).
The top gainers were (YIACO), (HCC), (NIH), (ZIMAH) and (KINV), while the top volume shares were (
ADNC), (INVESTORS), (ALMUDON), (MANAZEL) and (GPI).(end) ag