Sections were washed in phosphate-buffered saline-Tween and counterstained with
DAPI. Antifade mounting medium (Beyotime, Haimen, China) was added.
Indices
DAPI and PI correspond to the two fluorescent dyes used.
Then, nuclei were stained using the kit CyStain[R] UV Precise P (Partec GmbH., Munster, Germany) with 1600 [micro]l of
DAPI (1x) solution.
We used a 405-nm laser to illuminate
DAPI, a 488-nm laser for Alexa-flour (EdU), and a 594-nm laser for the natural fluorescence of Symbiodinium.
The chromosomes were counter-stained with
DAPI (0.2 mg per mL) in Vectashield Mountng Medium (Vector Laboratories, Burlingame, California, USA).
After 60 min, the cells were fixed with 4% paraformaldehyde and stained with
DAPI and fluorescein isothiocyanate- (FITC-) conjugated phalloidin.
Slides preparations were subjected to RNAse and Proteinase K pretreatments, followed by steps of denaturalization, probe hybridization, blocking, probe detection by means of antibodies linked to fluorochromes [anti-digoxigenin to fluorescein (FITC) and anti-biotin to rhodamine (TRITC)] (Dako, USA), washing and
DAPI staining for contrast.
Nuclei were counterstained with
DAPI (Blue channel).
Nova Lite
DAPI ANCA Kits are the first FDA cleared reagents for the detection of anti-neutrophil cytoplasmic antibodies on an automated digital IFA microscope, a long-awaited solution for laboratories performing ANCA IFA testing.
Por lo tanto, no se conoce la utilidad del bandeo
DAPI en el analisis cariotipico de las especies de Canna.