Third, the TCL-1 gene was expressed under the
EF1a promoter in our model.
ACTB, together with GAPDH, TUB,
EF1A and 18S rRNA, are expressed constitutively and are involved in basic housekeeping functions required for cell maintenance.
(2006) with three genes: leucine tRNA, COII mtDNA, and EF1a.
Downie and Gullan (2004) assessed the phylogeny of the Pseudococcidae using DNA sequences of EF1a, 28S and 18S.
Several alternative, broadly conserved, and phylogenetically useful gene sequences have also been explored as bacterial species markers, including 23S rDNA, internal transcribed spacer regions, hsp65, rpoB, recA, sodA, gyrB, IF2, and
EF1A. Once the sequence region of interest is amplified from crude or purified lysates of the cultured bacterial organisms, the sequence of the amplicons contains the information necessary to identify the underlying species.
All the sequences generated in the present study, corresponding to COI, COII and EF1a was aligned using BioEdit.
Comparison of the COI, COII and EF1a sequences of 22 populations of A.
Expected used size(bp)
ef1a 5'-GGCTGACTGTGCTGTGCTGATTG-3' 28 382 5' -CTTGTCGGTGGGACGGCTACG-3' vegfa 5'-GAGAGCCAGCGACTCACCGCAACAC-3' 28 623 for [vegfa.sub.165] 5-GTTCGCTCGATCATCATCTTGGC-3' 491 for [vegfa.sub.121] kdr 5' -GCCTGATCCACAACTGCTTCC-3' 31 150 5' -CTCTCCTCACACGACTCAATGC-3' angpt 1 5' -ACAGCAGTGGAACCGAACAG-3' 30 169 5'-AGCCTCCGCCAGCAGAC-3' angpt2 5' -AGGTGGAGGCTGGACTGTC-3' 28 141 5'-GTGGTGAGCAGGTGGATGAC-3' tie 1 5'-GCGATGGATGGCTATTGAGTCTCTA-3' 31 348 5' -GCATCTATTCCAGCATA-3' tie2 5' -CTACCCAGTGACCAACGC-3' 31 117 5'-GCTCTACAGCTCCTGACGAT-3' Gene Accession no.
Apart from this, various molecular markers have been employed for species identification and molecular phylogeny studies of insects, viz., Cytochrome b, 16S rRNA (von Dohlen & Moran 2000), 18S rRNA, 28S rRNA, 5.8S rRNA, ITS-Internal Transcribed Spacers, and
EF1a (Elongation Factor) (Ji et al.