Caption: Figure 4: The cytoplasm of these malignant cells stain positively for mucicarmine, thereby highly indicating an adenocarcinoma, not otherwise specified.
Percutaneous biopsy of the right subscapularis mass was performed demonstrating positive immunostaining with p63 and mucicarmine in a pattern similar to the penile masses.
Samples were then processed routinely and stained with hematoxylin and eosin, in addition to acid mucicarmine and toluidine blue stains to demonstrate mucin production.
MEC is usually composed of a mixture of predominantly epidermoid (squamoid) cells, abundant intermediate cells ranging from small basal cells with basophilic cytoplasm to larger cells with eosinophilic cytoplasm, and mucous cells which have a positive PAS reaction by staining with mucicarmine or Alcian blue [13].
The sections were stained with Mayer's haematoxylin and eosin stain for histomorphological studies (Luna, 1968), Masson's Trichrome stain for collagen fibers (Luna), Gridley's stain for reticular fibres (Sheehan & Hrapchak, 1973), Verhoeff's for elastic fibers (Sheehan & Hrapchak), Alcian blue for mucosubstances (pH 2.5), McManus' PAS method for glycogen, Mayer's mucicarmine method for mucin, and Sudan black B method for fats (Luna).