epitope

Antibody paratope characterization pertains a crucial step of generating a perfect antibody.

If the primary antibody can specifically bind its antigen in this pre-incubation, the antibody's paratope will be masked, preventing it from subsequently binding to epitopes in the tissue.

In response to antigenic stimuli and costimulatory signals from other immune cells, B cells undergo CSR to alter their Ig constant heavy chain while retaining the same antigen specificity, in effect, generating an alternative Ig isotype with the same paratope but distinct effector functions [87].

For example, Rerosa Federico, Favoino Elvira, Antonietta have reported that murine anti-idiotypic monoclonal antibodies 16D7 are able to recognize HP2/6 paratope of monoclonal antibodies (moAbs) against synthetic human CD4 molecules.

This presentation will discuss how HDX-MS can be used to observe conformational changes, includes the case study of a protein-substrate interaction and the case study of the identification of both the epitope and paratope of an antibody.

It is possible that certain conformational changes in the antibody paratope regions and/or adjustment of the 3-D shape of the drug might have been induced to accommodate both antibodies during the sandwich formation.

An antibody's paratope can bind with an antigen's epitope according to their affinity.

In this paper, a paratope with a built in robot's steering direction is regarded as antibody.