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Clostridium butyricum activates TLR2-mediated MyD88-independent signaling pathway in HT-29 cells

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Abstract

Oral administration of Clostridium butyricum as probiotic is increasingly gaining importance in the treatment of diarrhea and the improvement of animal performance. However, the mechanisms of host cell receptor recognition of C. butyricum and the downstream immune signaling pathways leading to these benefits remain unclear. The objective of this study was to analyze the mechanisms involved in C. butyricum induction of the toll-like receptor (TLR) signaling. Knockdown of myeloid differentiation primary response protein 88 (MyD88) expression using small interfering RNA in this manner did not affect C. butyricum-induced elevated levels of nuclear factor κB (NF-κB), interleukin-8 (IL-8), IL-6, and tumor necrosis factor alpha (TNF-α), suggesting a MyD88-independent route to TLR signaling transduction. However, a significant reduction in the levels of NF-κB, IL-8, IL-6, and TNF-α was evident in the absence of TLR2 expression, implying the need for TLR2 in C. butyricum recognition. Hence, C. butyricum activates TLR2-mediated MyD88-independent signaling pathway in human epithelial cells, which adds to our understanding of the molecular mechanisms of this probiotic action on gut epithelium.

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Abbreviations

PRRs:

Pattern recognition receptors

TLR:

Toll-like receptor

MyD88:

Myeloid differentiation primary response protein 88

TRIF:

Toll/IL-1 receptor-containing adapter inducing IFN-β

NF-κB:

Nuclear factor-κB

IκB:

Inhibitor of nuclear factor-κB

IL-8:

Interleukin-8

TNF-α:

Tumor necrosis factor alpha

SiRNA:

Small interfering RNA

ELISA :

Enzyme-linked immunosorbent assay

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Acknowledgment

This study was supported by National Natural Science Foundation of China (Grant No. 30901039) and Ningbo City Bureau of Science and Technology (Grant No. 2009A610155).

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Authors and Affiliations

  1. Key Laboratory for Molecular Design and Nutrition Engineering, Ningbo Institute of Technology, Zhejiang University, 1 Qianhunan Road, Ningbo, 315100, China

    Quanxin Gao, Lili Qi & Jinbo Wang

  2. College of Animal Sciences, Zhejiang University, Hangzhou, 310000, China

    Quanxin Gao & Tianxing Wu

Authors
  1. Quanxin Gao
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  2. Lili Qi
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  3. Tianxing Wu
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  4. Jinbo Wang
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Correspondence to Jinbo Wang.

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Gao, Q., Qi, L., Wu, T. et al. Clostridium butyricum activates TLR2-mediated MyD88-independent signaling pathway in HT-29 cells. Mol Cell Biochem 361, 31–37 (2012). https://doi.org/10.1007/s11010-011-1084-y

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  • DOI: https://doi.org/10.1007/s11010-011-1084-y

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