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Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine

Nature volume 283, pages 826–831 (1980)Cite this article

Abstract

The Abelson murine leukaemia virus protein (P120) can become phosphorylated in vitro by [γ-32P]ATP. The protein has been purified from cell membranes to the point that in specific conditions virtually all of the incorporated 32P is in P120. The reaction is stimulated by Mn2+ and Mg2+ but not Ca2+ and is very rapid even at 0 °C. The phosphate is linked to P120 at tyrosine, a linkage not previously reported for a phosphorylation reaction. Phosphorylation may be involved in the transforming activity of viruses that cause leukaemia as well as sarcomas.

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Authors and Affiliations

  1. Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    Owen N. Witte, Asim Dasgupta & David Baltimore

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  1. Owen N. Witte
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  2. Asim Dasgupta
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Witte, O., Dasgupta, A. & Baltimore, D. Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine. Nature 283, 826–831 (1980). https://doi.org/10.1038/283826a0

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