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Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin

Nature volume 324, pages 73–76 (1986)Cite this article

Abstract

When stimulated with antigen, B cells are influenced by T cells to proliferate and differentiate into antibody-forming cells. Since it was reported1,2 that soluble factors could replace certain functions of helper T cells in the antibody response, several different kinds of lymphokines and monokines have been reported in B-cell growth and differentiation3,4. Among these, human B-cell differentiation factor (BCDF or BSF-2) has been shown to induce the final maturation of B cells into immunoglobulin-secreting cells5–8. BSF-2 was purified to homogeneity9 and its partial NH2-terminal amino-acid sequence was determined10. These studies indicated that BSF-2 is functionally and structurally unlike other known proteins. Here, we report the molecular cloning, structural analysis and functional expression of the cDNA encoding human BSF-2. The primary sequence of BSF-2 deduced from the cDNA reveals that BSF-2 is a novel interleukin consisting of 184 amino acids.

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References

  1. Dutton, R. W. et al. Prog. Immun. 1, 355–368 (1971).

    Article  Google Scholar 

  2. Schimpl, A. & Wecker, E. Nature 237, 15–17 (1972).

    CAS  Google Scholar 

  3. Howard, M. & Paul, W. E. A. Rev. Immun. 1, 307–333 1983).

    Article  CAS  Google Scholar 

  4. Kishimoto, T. A. Rev. Immun. 3, 135–159 (1985).

    Article  Google Scholar 

  5. Okada, M. et al. J. exp. Med. 157, 583–590 (1983).

    Article  CAS  Google Scholar 

  6. Butler, J. L., Falkoff, R. J. M. & Fauci, A. S. Proc. natn. Acad. Sci. U.S.A. 81, 2475–2478 (1984).

    Article  ADS  CAS  Google Scholar 

  7. Hirano, T., Teranishi, T. Lin, B. H. & Onoue, K. J. Immun. 133, 798–802 (1984).

    CAS  PubMed  Google Scholar 

  8. Kikutani, H. et al. J. Immun. 134, 990–995 (1985).

    CAS  PubMed  Google Scholar 

  9. Hirano, T. et al. Proc. natn. Acad. Sci. U.S.A. 82, 5490–5494 (1985).

    Article  ADS  CAS  Google Scholar 

  10. Hirano, T. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  11. Shimizu, K. et al. J. Immun. 133, 1728–1733 (1985).

    Google Scholar 

  12. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  13. Maxam, A. W. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980)

    Article  CAS  Google Scholar 

  14. Nagata, S. et al. Nature 319, 415–418 (1986).

    Article  ADS  CAS  Google Scholar 

  15. Rawle, F.C. et al. Eur. J. Immun. 16, 1017–1019 (1986).

    Article  CAS  Google Scholar 

  16. Sutton, M. G. S. J., Mercier, L., Giuliani, E. R. & Lie, J. T. Mayo clin. Proc. 55, 371–376 (1980).

    Google Scholar 

  17. Hewick, R. M., Hunkapillar, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1981).

    CAS  PubMed  Google Scholar 

  18. Tsunasawa, S., Kondo, J. & Sakiyama, F. J. Biochem, Tokyo 97, 701–704 (1985).

    Article  CAS  Google Scholar 

  19. Taniguchi, T. et al. Nature 302, 305–310 (1983).

    Article  ADS  CAS  Google Scholar 

  20. Mizuuchi, K., Mizuuchi, M. & Gellert, M. J. molec. Biol. 156, 229–243 (1982).

    Article  CAS  Google Scholar 

  21. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    Article  CAS  Google Scholar 

  22. Huynh, T. V., Young, R. A. & Davis, R. W. DNA Cloning. Vol. 1, 49–78 (IRL press, Oxford, 1985).

    Google Scholar 

  23. Wood, W. I., Gitshier, J., Lasky, L. A. & Lawn, R. M. Proc. natn. Acad. Sci. U.S.A. 82, 1585–1588 (1985).

    Article  ADS  CAS  Google Scholar 

  24. Gluzman, Y. Cell 23, 175–182 (1981).

    Article  CAS  Google Scholar 

  25. Wilbur, W. J. & Lipman, D. J. Proc. natn. Acad. Sci. U.S.A. 80, 726–730 (1983).

    Article  ADS  CAS  Google Scholar 

  26. Lehrach, H., Diamond, D., Wozey, J. M. & Boedtker, H. Biochemistry 16, 4743–4751 (1977).

    Article  CAS  Google Scholar 

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Author notes

  1. Hisashi Harada and Tadatsugu Taniguchi: Division of Molecular Biology, Institute for Molecular and Cellular Biology, Osaka University, 1–3, Yamada-Oka, Suita, Osaka 565, Japan

  2. Akihiro Iwamatsu, Susumu Tsunasawa and Fumio Sakiyama: Division of Protein Chemistry, Institute for Protein Research, Osaka University, 1–3, Yamada-Oka, Suita, Osaka 565, Japan

  3. Hiroshi Matsui and Yoshiyuki Takahara: Central Research Laboratories, Ajinomoto Co. Inc., Totsuka-ku, Yokohama 244, Japan

Authors and Affiliations

  1. Division of Immunology, Osaka University, 1–3, Yamada-Oka, Suita, Osaka, 565, Japan

    Toshio Hirano, Kiyoshi Yasukawa, Hisashi Harada, Tetsuya Taga, Yasuo Watanabe, Tadashi Matsuda, Shin-ichiro Kashiwamura, Koichi Nakajima, Koichi Koyama, Akihiro Iwamatsu, Susumu Tsunasawa, Fumio Sakiyama, Hiroshi Matsui, Yoshiyuki Takahara, Tadatsugu Taniguchi & Tadamitsu Kishimoto

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  1. Toshio Hirano
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  2. Kiyoshi Yasukawa
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  7. Shin-ichiro Kashiwamura
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  8. Koichi Nakajima
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  9. Koichi Koyama
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  10. Akihiro Iwamatsu
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  11. Susumu Tsunasawa
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  13. Hiroshi Matsui
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  14. Yoshiyuki Takahara
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  15. Tadatsugu Taniguchi
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  16. Tadamitsu Kishimoto
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Hirano, T., Yasukawa, K., Harada, H. et al. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324, 73–76 (1986). https://doi.org/10.1038/324073a0

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