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Atomic structure of the actin: DNase I complex

Nature volume 347, pages 37–44 (1990)Cite this article

Abstract

The atomic models of the complex between rabbit skeletal muscle actin and bovine pancreatic deoxyribonuclease I both in the ATP and ADP forms have been determined byo X-ray analysis at an effective resolution of 2.8 Å and 3 Å, respectively. The two structures are very similar. The actin molecule consists of two domains which can be further subdivided into two subdomains. ADP or ATP is located in the cleft between the domains with a calcium ion bound to the β- or β- and γ-phosphates, respectively. The motif of a five-stranded (3 sheet consisting of a (3 meander and a right handed β α β unit appears in each domain suggesting that gene duplication might have occurred. These sheets have the same topology as that found in hexokinase.

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References

  1. Straub, F. B. Studies, University of Szeged II, 3–15 (1942).

    Google Scholar 

  2. Straub, F. B. Studies, University of Szeged III, 23–27 (1943).

    Google Scholar 

  3. Pollard, T. D. Curr. Opinion Cell Biol. 2, 33–40 (1990).

    Article  CAS  Google Scholar 

  4. Vandekerckhove, J. Curr. Opinion Cell Biol. 2, 41–50 (1990).

    Article  CAS  Google Scholar 

  5. Elzinga, M., Collins, J. H., Kuehl, W. M. & Adelstein, R. S. Proc. natn. Acad. Sci. U.S.A. 70, 2687–2691 (1973).

    Article  ADS  CAS  Google Scholar 

  6. Collins, J. H. & Elzinga, M. J. biol. Chem. 250, 5915–5920 (1975).

    CAS  PubMed  Google Scholar 

  7. Lu, R. C. & Elzinga, M. Biochemistry 16, 5801–5806 (1977).

    Article  CAS  Google Scholar 

  8. Vandekerckhove, J. & Weber, K. Eur. J. Biochem. 90, 451–462 (1978).

    Article  CAS  Google Scholar 

  9. Lazarides, E. & Lindberg, U. Proc. natn. Acad. Sci. U.S.A. 71, 4742–4746 (1974).

    Article  ADS  CAS  Google Scholar 

  10. Mannherz, H. G., Barrington-Leigh, J., Leberman, R. & Pfrang, H. FEBS Lett. 60, 34–38 (1975).

    Article  CAS  Google Scholar 

  11. Hitchcock, S. E. J. biol. Chem. 255, 5668–5673 (1980).

    CAS  PubMed  Google Scholar 

  12. Mannherz, H. G., Kabsch, W. & Leberman, R. FEBS Lett. 73, 141–143 (1977).

    Article  CAS  Google Scholar 

  13. Sugino H. et al. J. Biochem. 86, 257–260 (1979).

    CAS  PubMed  Google Scholar 

  14. Suck, D., Kabsch, W. & Mannherz, H. G. Proc. natn. Acad. Sci. U.S.A. 78, 4319–4323 (1981).

    Article  ADS  CAS  Google Scholar 

  15. Kabsch, W., Mannherz, H. G. & Suck, D. EMBO J. 4, 2113–2118 (1985).

    Article  CAS  Google Scholar 

  16. Suck, D., Oefner, C. & Kabsch, W. EMBO J. 3, 2423–2430 (1984).

    Article  CAS  Google Scholar 

  17. Oefner, C. & Suck, D. J. molec. Biol. 192, 605–632 (1986).

    Article  CAS  Google Scholar 

  18. Kabsch, W. J. appl. Crystallogr. 21, 916–924 (1988).

    Article  CAS  Google Scholar 

  19. Kabsch, W. J. appl. Crystallogr. 21, 67–71 (1988).

    Article  CAS  Google Scholar 

  20. Kabsch, W., Pai, E. F., Mannherz, H. G. & Suck, D. Springer Series in Biophysics (eds Aebi U. & Engel, J.) 3, 42–47 (1989).

    Google Scholar 

  21. Bruenger, A. T., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).

    Article  ADS  CAS  Google Scholar 

  22. Read, R. J. Acta crystallogr. A42, 140–149 (1986).

    Article  CAS  Google Scholar 

  23. Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).

    Article  CAS  Google Scholar 

  24. Steitz, T. A., Fletterick, R. J., Anderson, W. A. & Anderson, C. M. J. molec. Biol. 104, 197–222 (1976).

    Article  CAS  Google Scholar 

  25. Kabsch, W. & Sander, C. Biopolymers 22, 2577–2637 (1983).

    Article  CAS  Google Scholar 

  26. Naharro, G., Robbins, K. C. & Reddy, E. P. Science 223, 63–66 (1984).

    Article  ADS  CAS  Google Scholar 

  27. Holmes, K. C., Popp, D., Gebhard, W. & Kabsch, W., Nature 347, 44–49 (1990).

    Article  ADS  CAS  Google Scholar 

  28. Polzar, B., Nowak, E., Goody, R. S. & Mannherz, H. G. Eur. J. Biochem. 182, 267–275 (1989).

    Article  CAS  Google Scholar 

  29. Suck, D., Lahm, A. & Oefner, C. Nature 332, 464–468 (1988).

    Article  ADS  CAS  Google Scholar 

  30. Boyer, M., Roustan, C. & Benyamin, Y. Biosci. Rpt 5, 39–46 (1985).

    Article  CAS  Google Scholar 

  31. Sutoh, K. Biochemistry 23, 1942–1946 (1984).

    Article  CAS  Google Scholar 

  32. Mornet, D. & Ue, K. Proc. natn. Acad. Sci. U.S.A. 81, 3680–3684 (1984).

    Article  ADS  CAS  Google Scholar 

  33. Mornet, D., Bonet, A., Audemard, E. & Bonicel, J. J. Muscle Res. Cell Motil. 10, 10–24 (1989).

    Article  CAS  Google Scholar 

  34. Jacobson, G. R. & Rosenbusch, J. P. Proc. natn. Acad. Sci. U.S.A. 73, 2742–2746 (1976).

    Article  ADS  CAS  Google Scholar 

  35. Johnson, P., Wester, P. J. & Hikida, R. S. Biochim. biophys. Acta 578, 253–257 (1979).

    Article  CAS  Google Scholar 

  36. Konno, K. J. Biochem., Tokyo 103, 386–392 (1988).

    Article  CAS  Google Scholar 

  37. Schwyter, D., Phillips, M. & Reisler, E. Biochemistry 28, 5889–5895 (1989).

    Article  CAS  Google Scholar 

  38. Carlsson, L. et al. J. molec. Biol. 105, 353–366 (1976).

    Article  CAS  Google Scholar 

  39. Schutt, C. E., Lindberg, U., Myslik, J. & Strauss, N. J. molec. Biol. 209, 735–746 (1989).

    Article  CAS  Google Scholar 

  40. Vandekerckhove, J. S., Kaiser, D. A. & Pollard, T. D. J. Cell Biol. 109, 619–626 (1989).

    Article  CAS  Google Scholar 

  41. Kuwayama, H. & Yount, R. G. Biophys. J. 49, 454a (1986).

    Google Scholar 

  42. Hegyi, Szilagyi, L. & Elzinga, M. Biochemistry 25, 5793–5798 (1986).

    Article  CAS  Google Scholar 

  43. Saenger, W. Principles of Nucleic Acid Structure 74 (Springer-Verlag, 1984).

    Book  Google Scholar 

  44. DePhamphilis, M. L. & Cleland, W. W. Biochemistry 12, 3714–3723 (1973).

    Article  Google Scholar 

  45. Strzelecka-Golaszewska, H., Boguta, G., Zmorzynski, S. & Moraczewska, J. Eur. J. Biochem. 182, 299–305 (1989).

    Article  CAS  Google Scholar 

  46. Nowak, E., Strzelecka-Golaszewska, H. & Goody, R. S. Biochemistry 27, 1785–1792 (1988).

    Article  CAS  Google Scholar 

  47. Loscalzo, J. & Reed, G. H. Biochemistry, 15, 5407–5412 (1976).

    Article  CAS  Google Scholar 

  48. Brauer, M. & Sykes, B. D. Biochemistry 21, 5934–5939 (1982).

    Article  CAS  Google Scholar 

  49. Miki, M. & Wahl, P. Biochim. biophys. Acta 828, 188–195 (1985).

    Article  CAS  Google Scholar 

  50. Valentin-Ranc, C. & Carlier, M.-F. J. biol. Chem. 264, 20871–20880 (1989).

    CAS  PubMed  Google Scholar 

  51. Moore, P. B., Huxley, H. E. & DeRosier, D. J. J. molec. Biol. 50, 279–295 (1970).

    Article  CAS  Google Scholar 

  52. Sutoh, K. Biochemistry 21, 3654–3661 (1982).

    Article  CAS  Google Scholar 

  53. Bertrand, R. et al. Biochemistry 27, 5728–5736 (1988).

    Article  CAS  Google Scholar 

  54. Méjean, C. et al. Biosci. Rept 6, 493–499 (1986).

    Article  Google Scholar 

  55. Miller, L., Kalnowski, M., Yunossi, Z., Bulsinki, J. C. & Reisler, E. Biochemistry 26, 6064–6070 (1987).

    Article  CAS  Google Scholar 

  56. Moir, A. J. G., Levine, B. A., Goodearl, A. J. & Trayer, I. P. J. Muscle Res. Cell Motil. 8, 68–69 (1988).

    Google Scholar 

  57. Barden, J. A., Phillips, L., Cornell, B. A. & dos Remedios, C. G. Biochemistry 28, 5895–5901 (1989).

    Article  CAS  Google Scholar 

  58. Barden, J. A. & Phillips, L. Biochemistry 29, 1348–1354 (1990).

    Article  CAS  Google Scholar 

  59. Szilagyi, L. & Lu, R. C. Biochim. Biophys. Acta 709, 204–211 (1982).

    Article  CAS  Google Scholar 

  60. Trayer, I. P., Trayer, H. R. & Levine, B. A. Eur. J. Biochem. 164, 259–266 (1987).

    Article  CAS  Google Scholar 

  61. Flaherty, K. M., DeLuca-Flaherty, C. & McKay, D. B. Nature 346, 623–628 (1990).

    Article  ADS  CAS  Google Scholar 

  62. Blum, M., Metcalf, P. & Wiley, D. C. J. appl. Crystallogr. 20, 235–247 (1987).

    Article  CAS  Google Scholar 

  63. dos Remedios, C., Miki, M. & Barden, J. A. J. Muscle Res. Cell Motil. 8, 97–117 (1987).

    Article  CAS  Google Scholar 

  64. Priestle, J. P. J. appl. Crystallogr. 21, 572–576 (1988).

    Article  Google Scholar 

Download references

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Author notes

  1. Hans Georg Mannherz: Institut für Anatomie und Zellbiologie der Philipps-Universitat, Robert-Koch-StraBe 6, 3550 Marburg, FRG

  2. Dietrich Suck: European Molecular Biology Laboratory, Postfach 102209, 6900 Heidelberg, FRG

Authors and Affiliations

  1. Max-Planck-lnstitut für medizinische Forschung, Abteilung Biophysik, JahnstraBe 29, 6900, Heidelberg, FRG

    Wolfgang Kabsch, Hans Georg Mannherz, Dietrich Suck, Emil F. Pai & Kenneth C. Holmes

Authors
  1. Wolfgang Kabsch
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  2. Hans Georg Mannherz
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  3. Dietrich Suck
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  4. Emil F. Pai
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  5. Kenneth C. Holmes
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Kabsch, W., Mannherz, H., Suck, D. et al. Atomic structure of the actin: DNase I complex . Nature 347, 37–44 (1990). https://doi.org/10.1038/347037a0

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