Abstract
RNA enzymes are postulated to have catalysed all chemical reactions in the earliest living cells. This idea is now investigated in a search for alkyl transferases from a pool of random sequence RNAs. Selection for self-biotinylation yields a transfer RNA-like ribozyme that efficiently catalyses carbon–nitrogen bond formation. Ribozymes can thus promote reactions other than those involving the RNA sugar–phosphate backbone, suggesting that RNA may be capable of a broad range of catalytic activities.
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References
Joyce, G. F. Nature 338, 217–224 (1989).
Benner, S. A., Ellington, A. D. & Tauer, A. Proc. natn. Acad. Sci. U.S.A. 86, 7054–7058 (1989).
Wilson, C. & Szostak, J. W. Curr. Opin. struct. Biol. 2, 749–756 (1992).
Ellington, A. D. & Szostak, J. W. Nature 346, 818–822 (1990).
Famulok, M. & Szostak, J. W. J. Am. chem. Soc. 114, 3990–3991 (1992).
Sassanfar, M. & & Szostak, J. W. Nature 364, 550–553 (1993).
Connell, G. J., Illangesekare, M. & Yarus, M. Biochemistry 32, 5497–5502 (1993).
Lorsch, J. R. & Szostak, J. W. Biochemistry 33, 973–982 (1994).
Jenison, R. D., Gill, S. C., Pardi, A. & Polisky, B. Science 263, 1425–1429 (1994).
Famulok, M. J. Am. chem. Soc. 116, 1698–1706 (1994).
Bartel, D. P. & Szostak, J. W. Science 261, 1411–1418 (1993).
Lorsch, J. R. & Szostak, J. W. Nature 371, 31–36 (1994).
Prudent, J. R., Uno, T. & Schultz, P. G. Science 264, 1924–1927 (1994).
Green, N. M. Adv. Protein Chem. 29, 85–133 (1975).
Al-hakim, A. H. Biochem. J. 251, 935–938 (1988).
Peattie, D. A. Proc. natn. Acad. Sci. U.S.A. 76, 1760–1764 (1979).
Tramontano, A., Ammann, A. A. & Lerner, R. A. J. Am. chem. Soc. 110, 2282–2283 (1988).
Janda, K. D., Weinhouse, M. I., Schloeder, D. M., Lerner, R. A., & Benkovic, S. J. J. Am. chem. Soc. 112, 1274–1275 (1990).
Fersht, A. Enzyme Structure and Mechanism, 2nd edn 57–59 (Freeman, New York, 1985).
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Wilson, C., Szostak, J. In vitro evolution of a self-alkylatlng ribozyme. Nature 374, 777–782 (1995). https://doi.org/10.1038/374777a0
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DOI: https://doi.org/10.1038/374777a0
