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A dendritic-cell-derived C–C chemokine that preferentially attracts naive T cells

Nature volume 387, pages 713–717 (1997)Cite this article

Abstract

Dendritic cells form a system of highly efficient antigen-presenting cells. After capturing antigen in the periphery, they migrate to lymphoid organs where they present the antigen to T cells1,2. Their seemingly unique ability to interact with and sensitize naive T cells gives dendritic cells a central role in the initiation of immune responses and allows them to be used in therapeutic strategies against cancer, viral infection and other diseases. How they interact preferentially with naive rather than activated T lymphocytes is still poorly understood. Chemokines direct the transport of white blood cells in immune surveillance3,4. Here we report the identification and characterization of a C-C chemokine (DC-CK1) that is specifically expressed by human dendritic cells at high levels. Tissue distribution analysis demonstrates that dendritic cells present in germinal centres and T-cell areas of secondary lymphoid organs express this chemokine. We show that DC-CK1, in contrast to RANTES, MIP-1α and interleukin-8, preferentially attracts naive T cells (CD45RA+). The specific expression of DC-CK1 by dendritic cells at the site of initiation of an immune response, combined with its chemotactic activity for naive T cells, suggests that DC-CK1 has an important rule in the induction of immune responses.

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Figure 1: a, Nucleotide and amino-acid sequence of DC-CK1.
Figure 2: DC-CK1 mRNA is specifically expressed by dendritic cells.
Figure 3: Detection of DC-CK1 in germinal centres and T-cell areas of a tonsil by in situ hybridization.
Figure 4: DC-CK1 is a potent chemoattractant for the CD45RA+T-cell subset.

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Acknowledgements

We thank S. Zurawski, D. Gorman, F. Vega, R. Kastelein, M. Bell, K. Franz-Bacon, D.Figueroa, M. Koningswieser, R. Huijbens, C. Maass and L. Schalkwijk for assistance, and G. Zurawski, D. Ruiter and P. de Mulder for support. The DNAX Research Institute is supported by Schering Ploung Corporation.

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Authors and Affiliations

  1. *Departments of Tumour Immunology, University Hospital Nijmegen St Radboud, Philips van Leydenlaan 25, 6525 EX, Nijmegen, The Netherlands

    Gosse J. Adema, Franca Hartgers, Riet Verstraten, Edwin de Vries, Gill Marland & Carl G. Figdor

  2. ‡Department of Pathology, University Hospital Nijmegen St Radboud, Philips van Leydenlaan 25, 6525 EX, Nijmegen, The Netherlands

    Pete Nooyen

  3. †Departments of Immunology and Molecular Biology, DNAX Research Institute, Palo Alto, 94304, California, USA

    Satish Menon, Jessica Foster, Yuming Xu, Terrill McClanahan & Kevin B. Bacon

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  1. Gosse J. Adema
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Correspondence to Gosse J. Adema.

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Adema, G., Hartgers, F., Verstraten, R. et al. A dendritic-cell-derived C–C chemokine that preferentially attracts naive T cells. Nature 387, 713–717 (1997). https://doi.org/10.1038/42716

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