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Targeted gene disruption in somatic zebrafish cells using engineered TALENs

Nature Biotechnology volume 29, pages 697–698 (2011)Cite this article

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To the Editor:

Miller et al.1 recently described a transcription activator–like effector nuclease (TALEN) architecture for efficient genome editing in cultured human cells. We sought to determine whether the same framework could be used to efficiently disrupt endogenous genes in somatic cells of zebrafish and how the efficiency of TALENs compares with that obtained using engineered zinc-finger nucleases (ZFNs).

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Figure 1: Target sequences, frequencies of mutations and mutations induced by TALENs in embryonic zebrafish cells.

References

  1. Miller, J.C. et al. Nat. Biotechnol. 29, 143–148 (2011).

    Article  PubMed  Google Scholar 

  2. Moscou, M.J. & Bogdanove, A.J. Science 326, 1501 (2009).

    Article  PubMed  Google Scholar 

  3. Boch, J. et al. Science 326, 1509–1512 (2009).

    Article  PubMed  Google Scholar 

  4. Cermak, T. et al. Nucleic Acids Res. 39, e82 (2011).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Meng, X., Noyes, M.B., Zhu, L.J., Lawson, N.D. & Wolfe, S.A. Nat. Biotechnol. 26, 695–701 (2008).

    Article  PubMed  PubMed Central  Google Scholar 

  6. Doyon, Y. et al. Nat. Biotechnol. 26, 702–708 (2008).

    Article  PubMed Central  Google Scholar 

  7. Foley, J.E. et al. PLoS ONE 4, e4348 (2009).

    Article  PubMed  PubMed Central  Google Scholar 

  8. Cifuentes, D. et al. Science 328, 1694–1698 (2010).

    Article  PubMed  PubMed Central  Google Scholar 

  9. Siekmann, A.F., Standley, C., Fogarty, K.E., Wolfe, S.A. & Lawson, N.D. Genes Dev. 23, 2272–2277 (2009).

    Article  PubMed  PubMed Central  Google Scholar 

  10. Sander, J.D. et al. Nat. Methods 8, 67–69 (2011).

    Article  PubMed  Google Scholar 

  11. Miller, J.C. et al. Nat. Biotechnol. 25, 778–785 (2007).

    Article  PubMed  Google Scholar 

  12. Wood, A.J. et al. 333, 307 (2011).

Download references

Acknowledgements

We thank D. Voytas and A. Bogdanove for helpful discussions in the early stages of this project and J. Foley, M. Maeder, S. Thibodeau-Beganny, F. Zhang, M. Christian and D. Voytas for help with characterizing the hey2- and gria3a-targeted ZFN pairs. This work was supported by US National Institutes of Health (NIH) R01 GM088040 (J.K.J. & R.T.P.), NIH Director's Pioneer Award DP1 OD006862 (J.K.J.), NIH T32 CA009216 (J.D.S.), NIH K01 AG031300 (J.-R.J.Y.), the Jim and Ann Orr Massachusetts General Hospital (MGH) Research Scholar award (J.K.J.), and the Charles and Ann Sanders MGH Research Scholar award (R.T.P.).

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Author notes

  1. Jeffry D Sander and Lindsay Cade: These authors contributed equally to this work.

Authors and Affiliations

  1. Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA

    Jeffry D Sander, Cyd Khayter & J Keith Joung

  2. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA

    Jeffry D Sander & J Keith Joung

  3. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA

    Lindsay Cade, Randall T Peterson & Jing-Ruey J Yeh

  4. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA

    Lindsay Cade, Randall T Peterson & Jing-Ruey J Yeh

  5. Department of Genetics, Development and Cell Biology,

    Deepak Reyon

  6. Interdepartmental Graduate Program in Bioinformatics & Computational Biology, Iowa State University, Ames, Iowa, USA

    Deepak Reyon

  7. Broad Institute, Cambridge, Massachusetts, USA

    Randall T Peterson

Authors
  1. Jeffry D Sander
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  2. Lindsay Cade
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  3. Cyd Khayter
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  6. J Keith Joung
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Corresponding authors

Correspondence to J Keith Joung or Jing-Ruey J Yeh.

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Supplementary Figures 1–12, Supplementary Table 1 and Supplementary Methods (PDF 1457 kb)

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Sander, J., Cade, L., Khayter, C. et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol 29, 697–698 (2011). https://doi.org/10.1038/nbt.1934

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