Abstract
We have constructed a genetic map of the mouse genome containing 4,006 simple sequence length polymorphims (SSLPs). The map provides an average spacing of 0.35 centiMorgans (cM) between markers, corresponding to about 750 kb. Approximately 90% of the genome lies within 1.1 cM of a marker and 99% lies within 2.2 cM. The markers have an average polymorphism rate of 50% in crosses between laboratory strains. The markers are distributed in a relatively uniform fashion across the genome, although some deviations from randomness can be detected. In particular, there is a significant underrepresentation of markers on the X chromosome. This map represents the two–thirds point toward our goal of developing a mouse genetic map containing 6,000 SSLPs.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
206,07 € per year
only 17,17 € per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Weber, J.L. & May, P.E. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. hum. Genet. 44, 388–396 (1989).
Love, J.M., Knight, A.M., McAleer, M.A. & Todd, J.A. Towards construction of a high-resolution map of the mouse genome using PCR analysed microsatellites. Nucl. Acids Res. 18, 4123–4130 (1990).
Weissenbach, J. et al. A second-generation linkage map of the human genome. Nature 359, 794–801 (1992).
Dietrich, W.F. et al. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447 (1992).
Gypay, G. et al. The 1993–94 Gménéthon human genetic linkage map. Nature Genet. 7, 246–339 (1994).
Dietrich, W.F. et al. in Genetic Maps 1992 (ed. O'Brien, S.) 4.110–4.142 (Cold Spring Harbor Laboratory Press, New York, 1992).
Miller, J.C. et al. in Genetic Variants and Strains of the Laboratory Mouse 3rd edn (eds Lyons, M. F. & Searle, A.) (Oxford University Press, New York, in the press).
Copeland, N.G. et al. A genetic linkage map of the mouse: Current applications and future prospects. Science 262, 57–66 (1993).
Copeland, N.G. et al. Genome Maps IV: The Mouse. Science 262, 67–82 (1993).
Evans, E. in Genetic Variants and Strains of the Laboratory Mouse 2nd edn (eds Lyon, M. F. & Searle, A.) 576–578 (Oxford University Press, New York, 1989).
Mardon, G. et al. Mouse Zfx protein is similar to Zfy-2: each contains an acidic activating domain and 13 zinc fingers. Molec. Cell Biol. 10, 681–688 (1990).
Arratia, R., Goldstein, L. & Gordon, L. Poisson Approximation and the Chen-Stein Method. Stat. Sci. 5, 403–134 (1990).
Eicher, E.M. & Shown, E.P. Molecular markers that define the distal ends of mouse autosomes 4, 13, 19 and the sex chromosomes. Mamm. Genome. 4, 226–229 (1993).
Tanzi, R.E. et al. A genetic linkage map of human chromosome 21: analysis of recombination as a function of sex and age. Am. J. hum. Genet. 50, 551–558 (1992).
Bryda, E.C., De Pari, J.A., Sant'Angelo, D.B., Murphy, D.B. & Passmore, H.C. Multiple sites of crossing over within the Eb recombinational hotspot in the mouse. Mamm. Genome 2, 123–129 (1992).
Hofker, M.H. et al. The X chromosome shows less genetic variation at restriction sites than the autosomes. Am. J. hum. Genet. 3, 438–451 (1986).
Lander, E.S. & Botstein, D. Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121, 185–199 (1989).
Michelmore, R.W., Paran, I. & Kesseli, R.V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. natn. Acad. Sci. U.S.A. 88, 9828–32 (1991).
Larin, Z., Monaco, A.P. & Lehrach, H. Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc. natn. Acad. Sci. U.S.A. 88, 4123 (1991).
Kusumi, K., Smith, U.S., Segre, J.A., Koos, D.S. & Lander, E.S. Construction of a large-insert yeast artificial chromosome (YAC) library of the mouse genome. Mamm. Genome 4, 391–392 (1993).
Bruford, M.W. & Wayne, R.K. Microsatellites and their application to population genetic studies. Curr. Op. Genet. Devel. 3, 939–944 (1993).
Lander, E.S. et al. MAPMAKER: An interactive computer package for constructing genetic linkage maps of experimental and natural populations. Genomics 1, 174–181 (1987).
Lincoln, S.E. & Lander, E.S. Systematic detection of errors in genomic linkage data. Genomics 14, 604–610 (1992).
Hawkins, T. M13 single-strand purification using a biotinylated probe and streptavidin coated magnetic beads. DNA Seq. 3, 65–69 (1992).
Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual 2 edn (Cold Spring Harbor Laboratory, New York, 1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dietrich, W., Miller, J., Steen, R. et al. A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nat Genet 7 (Suppl 2), 220–245 (1994). https://doi.org/10.1038/ng0694supp-220
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/ng0694supp-220
This article is cited by
-
Genome-wide characterization of microsatellite DNA in fishes: survey and analysis of their abundance and frequency in genome-specific regions
BMC Genomics (2021)
-
Fine mapping of the major bleomycin-induced pulmonary fibrosis susceptibility locus in mice
Mammalian Genome (2018)
-
The Bermuda Triangle: The Pragmatics, Policies, and Principles for Data Sharing in the History of the Human Genome Project
Journal of the History of Biology (2018)
-
Differential effects of genes of the Rb1 signalling pathway on osteosarcoma incidence and latency in alpha-particle irradiated mice
Radiation and Environmental Biophysics (2011)
-
Mutational Dynamics of Microsatellites
Molecular Biotechnology (2010)
