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Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells

Nature Genetics volume 24, pages 372–376 (2000)Cite this article

Abstract

Cell fate during development is defined by transcription factors that act as molecular switches to activate or repress specific gene expression programmes. The POU transcription factor Oct-3/4 (encoded by Pou5f1) is a candidate regulator in pluripotent and germline cells1,2,3,4 and is essential for the initial formation of a pluripotent founder cell population in the mammalian embryo5. Here we use conditional expression and repression in embryonic stem (ES) cells to determine requirements for Oct-3/4 in the maintenance of developmental potency. Although transcriptional determination has usually been considered as a binary on-off control system, we found that the precise level of Oct-3/4 governs three distinct fates of ES cells. A less than twofold increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct-3/4 induces loss of pluripotency and dedifferentiation to trophectoderm. Thus a critical amount of Oct-3/4 is required to sustain stem-cell self-renewal, and up- or downregulation induce divergent developmental programmes. Our findings establish a role for Oct-3/4 as a master regulator of pluripotency that controls lineage commitment and illustrate the sophistication of critical transcriptional regulators and the consequent importance of quantitative analyses.

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Figure 1: Generation of ES cells with variable Oct-3/4 expression.
Figure 2: Differentiation of ZHTc6 and ZHBTc4 ES cells induced by modulation of Oct-3/4 expression.
Figure 3: Morphological differentiation of ZHBTc4 ES cells on repression of Oct-3/4.
Figure 4: Comparison of gene-expression pattern during differentiation of ZHBTc4 and ZHTc6 ES cells.
Figure 5: TS cell derivation from ZHBTc4 ES cells.
Figure 6: Functions of Oct-3/4 in pluripotent stem cells.

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References

  1. Schöler, H.R., Ruppert, S., Suzuki, N., Chowdhury, K. & Gruss, P. New type of POU domain in germ line-specific protein Oct-4. Nature 344, 435– 439 (1990).

    Article  Google Scholar 

  2. Rosner, M.H. et al. A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature s345, 686–692 (1990).

    Article  Google Scholar 

  3. Okamoto, K. et al. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell 60, 461–472 (1990).

    Article  CAS  Google Scholar 

  4. Pesce, M., Wang, X., Wolgemuth, D.J. & Scholer, H. Differential expression of the Oct-4 transcription factor during mouse germ cell differentiation. Mech. Dev. 71, 89– 98 (1998).

    Article  CAS  Google Scholar 

  5. Nichols, J. et al. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct-4. Cell 95, 379–391 (1998).

    Article  CAS  Google Scholar 

  6. Gossen, M. & Bujard, H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl Acad. Sci. USA 89, 5547–5551 (1992).

    Article  CAS  Google Scholar 

  7. Mountford, P. et al. Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression. Proc. Natl Acad. Sci. USA 91, 4303–4307 (1994).

    Article  CAS  Google Scholar 

  8. Gassmann, M., Donoho, G. & Berg, P. Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells. Proc. Natl Acad. Sci. USA 92, 1292–1296 (1995).

    Article  CAS  Google Scholar 

  9. Niwa, H., Burdon, T., Chambers, I. & Smith, A.G. Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev. 12, 2048–2060 (1998).

    Article  CAS  Google Scholar 

  10. Smith, A. G. et al. Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 336 , 688–690 (1988).

    Article  CAS  Google Scholar 

  11. Saijoh, Y. et al. Identification of putative downstream genes of Oct-3, a pluripotent cell-specific transcription factor. Genes Cells 1, 239–252 (1996).

    Article  CAS  Google Scholar 

  12. Arceci, R.J., King, A.A., Simon, M.C., Orkin, S.H. & Wilson, D.B. Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart. Mol. Cell. Biol. 13, 2235– 2246 (1993).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Wilkinson, D.G., Bhatt, S. & Herrmann, B.G. Expression pattern of the mouse T gene and its role in mesoderm formation. Nature 343, 657– 659 (1990).

    Article  CAS  Google Scholar 

  14. Nishikawa, S.I., Nishikawa, S., Hirashima, M., Matsuyoshi, N. & Kodama, H. Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. Development 125, 1747–1757 (1998).

    Article  CAS  Google Scholar 

  15. Hebert, J.M., Boyle, M. & Martin, G.R. mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation. Development 112, 407–415 (1991).

    Article  CAS  Google Scholar 

  16. Cserjesi, P., Brown, D., Lyons, G.E. & Olson, E.N. Expression of the novel basic helix-loop-helix gene eHAND in neural crest derivatives and extraembryonic membranes during mouse development. Dev. Biol. 170, 664–678 (1995).

    Article  CAS  Google Scholar 

  17. Beck, F., Erler, T., Russell, A. & James, R. Expression of Cdx-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev. Dyn. 204, 219–227 (1995).

    Article  CAS  Google Scholar 

  18. Brook, F.A. & Gardner, R.L. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl Acad. Sci. USA 94, 5709–5712 ( 1997).

    Article  CAS  Google Scholar 

  19. Beddington, R.S.P. & Robertson, E.J. An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo. Development 105, 733– 737 (1989).

    Article  CAS  Google Scholar 

  20. Tanaka, S., Kunath, T., Hadjantonakis, A.-K., Nagy, A. & Rossant, J. Promotion of trophoblast stem cell proliferation by FGF-4. Science 282, 2072 –2075 (1998).

    Article  CAS  Google Scholar 

  21. Ambrosetti, D.-C., Basilico, C. & Dailey, L. Synergistic activation of the fibroblast growth factor 4 enhancer by sox2 and oct-3 depends on protein-protein interactions facilitated by a specific spatial arrangement of factor binding sites. Mol. Cell. Biol. 17, 6321–6329 (1997).

    Article  CAS  Google Scholar 

  22. Nishimoto, M., Fukushima, A., Okuda, A. & Muramatsu, M. The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2. Mol. Cell. Biol. 19, 5453–5465 (1999).

    Article  CAS  Google Scholar 

  23. Schöler, H.R., Ciesiolka, T. & Gruss, P. Anexus between Oct-4 and E1A: implications for gene regulation in embryonic stem cells. Cell 66, 291–304 (1991).

    Article  Google Scholar 

  24. Ben-Shushan, E., Thompson, J.R., Gudas, L.J. & Bergman, Y. Rex-1, a gene encoding a transcription factor expressed in the early embryo, is regulated via Oct-3/4 and Oct-6 binding to an octamer site and a novel protein, Rox-1, binding to an adjacent site. Mol. Cell. Biol. 18, 1666–1878 (1998).

    Article  Google Scholar 

  25. Botquin, V. et al. New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2. Genes Dev. 12, 2073–2090 (1998).

    Article  CAS  Google Scholar 

  26. Burdon, T., Chambers, I., Niwa, H., Stracey, C. & Smith, A.G. Signalling mechanisms regulating self-renewal and differentiation of pluripotent embryonic stem cells. Cells Tissues Organs 165, 131–143 ( 1999).

    Article  CAS  Google Scholar 

  27. Dani, C. et al. Paracrine induction of stem cell renewal by LIF-deficient cells: a new ES cell regulatory pathway. Dev. Biol. 203, 149–162 (1998).

    Article  CAS  Google Scholar 

  28. Palmieri, S.L., Peter, W., Hess, H. & Schöler, H.R. Oct-4 transcription factor is differentially expressed in the mouse embryo during establishment of the first two extraembryonic cell lineages involved in implantation. Dev. Biol. 166, 259–267 (1994).

    Article  CAS  Google Scholar 

  29. Niwa, H., Yamamura, K.-I. & Miyazaki, J.-I. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193–200 (1991).

    Article  CAS  Google Scholar 

  30. Shimazaki, T. et al. Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential. EMBO J. 12, 4489–4498 (1993).

    Article  CAS  Google Scholar 

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Acknowledgements

We thank S. Nishikawa for access to time-lapse recording facilities and H. Schöler and H. Hamada for anti-Oct-3/4 antisera. We acknowledge F. Stehnouse for technical assistance. This work was supported by the Biotechnology and Biological Sciences Research Council of the United Kingdom, The Ministry of Education, Science and Culture of Japan, and a Uehara Memorial Foundation Award to H.N.

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Authors and Affiliations

  1. Centre for Genome Research, The University of Edinburgh, Edinburgh, King's Buildings, UK

    Hitoshi Niwa & Austin G. Smith

  2. Department of Nutrition and Physiological Chemistry, Osaka University School of Medicine, Osaka, Japan

    Hitoshi Niwa & Jun-ichi Miyazaki

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  1. Hitoshi Niwa
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Correspondence to Hitoshi Niwa or Austin G. Smith.

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Niwa, H., Miyazaki, Ji. & Smith, A. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24, 372–376 (2000). https://doi.org/10.1038/74199

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