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Transient incorporation of native GluR2-lacking AMPA receptors during hippocampal long-term potentiation

Nature Neuroscience volume 9, pages 602–604 (2006)Cite this article

Abstract

Postnatal glutamatergic principal neuron synapses are typically presumed to express only calcium-impermeable (CI), GluR2-containing AMPARs under physiological conditions. Here, however, we demonstrate that long-term potentiation (LTP) in CA1 hippocampal pyramidal neurons causes rapid incorporation of GluR2-lacking calcium-permeable (CP)-AMPARs: CP-AMPARs are present transiently, being replaced by GluR2-containing AMPARs ∼25 min after LTP induction. Thus, CP-AMPARs are physiologically expressed at CA1 pyramidal cell synapses during LTP, and may be required for LTP consolidation.

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Figure 1: LTP causes the transient incorporation of inwardly rectifying AMPARs at synapses in CA1 pyramidal neurons.
Figure 2: Philanthotoxin selectively blocks EPSCs when applied 3 or 10 min, but not 20 min, after LTP induction.

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References

  1. Shi, S., Hayashi, Y., Esteban, J.A. & Malinow, R. Cell 105, 331–343 (2001).

    Article  CAS  Google Scholar 

  2. Hayashi, Y. et al. Science 287, 2262–2267 (2000).

    Article  CAS  Google Scholar 

  3. Terashima, A. et al. J. Neurosci. 24, 5381–5390 (2004).

    Article  CAS  Google Scholar 

  4. Kumar, S.S., Bacci, A., Kharazia, V. & Huguenard, J.R. J. Neurosci. 22, 3005–3015 (2002).

    Article  CAS  Google Scholar 

  5. Ju, W. et al. Nat. Neurosci. 7, 244–253 (2004).

    Article  CAS  Google Scholar 

  6. Bowie, D. & Mayer, M.L. Neuron 15, 453–462 (1995).

    Article  CAS  Google Scholar 

  7. Kamboj, S.K., Swanson, G.T. & Cull-Candy, S.G. J. Physiol. (Lond.) 486, 297–303 (1995).

    Article  CAS  Google Scholar 

  8. Koh, D.S., Burnashev, N. & Jonas, P. J. Physiol. (Lond.) 486, 305–312 (1995).

    Article  CAS  Google Scholar 

  9. Toth, K. & McBain, C.J. Nat. Neurosci. 1, 572–578 (1998).

    Article  CAS  Google Scholar 

  10. Toth, K., Suares, G., Lawrence, J.J., Philips-Tansey, E. & McBain, C.J. J. Neurosci. 20, 8279–8289 (2000).

    Article  CAS  Google Scholar 

  11. Bagal, A.A., Kao, J.P., Tang, C.M. & Thompson, S.M. Proc. Natl. Acad. Sci. USA 102, 14434–14439 (2005).

    Article  CAS  Google Scholar 

  12. Bellone, C. & Luscher, C. Eur. J. Neurosci. 21, 1280–1288 (2005).

    Article  Google Scholar 

  13. Liu, S.Q. & Cull-Candy, S.G. Nature 405, 454–458 (2000).

    Article  CAS  Google Scholar 

  14. Malenka, R.C. & Bear, M.F. Neuron 44, 5–21 (2004).

    Article  CAS  Google Scholar 

  15. Frey, U. & Morris, R.G. Nature 385, 533–536 (1997).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Supported by the Wellcome Trust (A.T., G.L.C. and J.T.R.I.), the Medical Research Council UK (G.L.C. and J.T.R.I.), the National Institute of Neurological Disorders and Stroke Intramural Program (J.T.R.I.) and the National Institute of Child Health and Human Development Intramural Program (K.A.P. and C.J.M.)

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Author notes

  1. Karen Plant and Kenneth A Pelkey: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Anatomy, MRC Centre for Synaptic Plasticity, University of Bristol, School of Medical Sciences, Bristol, BS8 1TD, UK

    Karen Plant, Zuner A Bortolotto, Daiju Morita, Akira Terashima, Graham L Collingridge & John T R Isaac

  2. Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development, 35 Convent Drive, Bethesda, 20892, Maryland, USA

    Kenneth A Pelkey & Chris J McBain

  3. Developmental Synaptic Plasticity Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 35 Convent Drive, Bethesda, 20892, Maryland, USA

    Daiju Morita & John T R Isaac

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  1. Karen Plant
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  2. Kenneth A Pelkey
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Correspondence to John T R Isaac.

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Supplementary information

Supplementary Fig. 1

LTP is NMDA receptor-dependent and PhTx blocks potentiated EPSCs when applied three minutes after LTP induction. (PDF 2037 kb)

Supplementary Methods (PDF 54 kb)

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Plant, K., Pelkey, K., Bortolotto, Z. et al. Transient incorporation of native GluR2-lacking AMPA receptors during hippocampal long-term potentiation. Nat Neurosci 9, 602–604 (2006). https://doi.org/10.1038/nn1678

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