miR-200b mediates post-transcriptional repression of ZFHX1B
- Nanna Rønbjerg Christoffersen1,
- Asli Silahtaroglu2,
- Ulf Andersson Ørom1,
- Sakari Kauppinen2, and
- Anders H. Lund1,3
- 1Biotech Research and Innovation Centre, DK-2200 Copenhagen, Denmark
- 2Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, DK-2200 Copenhagen N, Denmark
- 3Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
Abstract
MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFβ signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3′-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.
Keywords
Footnotes
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Reprint requests to: Anders H. Lund, Biotech Research & Innovation Centre, Ole Maaløes Vej 5, DK-2200 Copenhagen, Denmark; e-mail: anders.lund{at}bric.dk; fax: (+45) 35325669.
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Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.586807.
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- Received March 29, 2007.
- Accepted May 8, 2007.
- Copyright © 2007 RNA Society
