Rhodamine: Difference between revisions

Content deleted Content added
No edit summary
Line 40:
There are many rhodamine derivatives used for imaging purposes, for example the tetramethyl rhodamine derivatives TRITC and TAMRA, sulforhodamine 101 (and its sulfonyl chloride form Texas Red) and Rhodamine Red. TRITC is the base rhodamine molecule functionalized with an [[isothiocyanate]] group ('''-N=C=S'''), replacing a [[hydrogen]] atom on the bottom ring of the structure. This derivative is reactive towards [[amine]] groups on proteins inside cells. A [[succinimidyl-ester]] functional group attached to the rhodamine core, creating NHS-rhodamine, forms another common amine-reactive derivative.
 
Other derivatives of rhodamine include newer fluorophores such as [[Alexa (fluor)|Alexa 546, Alexa 555, and Alexa 633]] and [[DyLight Fluor|DyLight 549]], have been tailored for various chemical and biological applications where higher [[Photobleaching|photostability]], increased brightness, different spectral characteristics, or different attachment groups are needed.
 
==External links==