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m Alexa Fluor 555 is a cyanine dye, not a rhodamine dye. |
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The laser dye rhodamine 123 is also used in biochemistry to inhibit [[mitochondrion]] function. Rhodamine 123 seems to bind to the mitochondrion membranes and inhibit transport processes, especially the electron transport chain, thus slowing down [[inner respiration]]. It is a substrate of [[P-glycoprotein]] (Pgp), which is usually overexpressed in cancer cells. Recent reports indicate that rhodamine 123 may be also a substrate of multidrug resistance-associated protein (MRP), or more specifically, MRP1.
==Other
There are many rhodamine derivatives used for imaging purposes, for example Carboxytetramethylrhodamine (TAMRA), tetramethylrhodamine (TMR) and its isothiocyanate derivative (TRITC) and, [[sulforhodamine 101]] (and its sulfonyl chloride form [[Texas Red]]) and Rhodamine Red. TRITC is the base rhodamine molecule functionalized with an [[isothiocyanate]] group ('''-N=C=S'''), replacing a [[hydrogen]] atom on the bottom ring of the structure. This derivative is reactive towards [[amine]] groups on proteins inside cells. A [[succinimidyl-ester]] functional group attached to the rhodamine core, creating NHS-rhodamine, forms another common amine-reactive derivative.
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