Rhodamine is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as a dye and as a dye laser gain medium. It is often used as a tracer within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be measured easily and inexpensively with instruments called fluorometers.
Rhodamine dyes are generally toxic, and are soluble in water, methanol, and ethanol.
Rhodamine B
Molecular Formula: C28H31N2O3Cl
Molecular Weight: 479.02 grams per mole
CAS Number: 81-88-9
Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium.
Rhodamine B is tunable around 610 nm when used as a laser dye.
Rhodamine B is also called Rhodamine 610, Basic Violet 10, or C.I. 45170.
Rhodamine 6G
Molecular Formula: C28H31N2O3Cl
Molecular Weight: 479.02 g/mol
CAS Number: 989-38-8
Rhodamine 6G is often used in a laser dye pumped by the 2nd (532 nm) harmonic from a Nd:YAG laser since it has a remarkably high photostability, high quantum yield, low cost, and close proximty to the absorption maximum (approximately 530 nm). The lasing range is 555 to 585 nm with a maximum at 566 nm.
Rhodamine 6G is also called Rhodamine 590, R6G, Basic Rhodamine Yellow , or C.I. 45160.
External links
- Absorption and Emission Spectra of Rhodamine B
- Absorption and Emission Spectra of Rhodamine 6G
- Absorption and Emission Spectra of Rhodamine 123
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