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ENZYME

ENZYME entry: EC 2.1.1.394

Accepted Name
2-(S-pantetheinyl)-carbapenam-3-carboxylate methyltransferase
Reaction catalysed
  • (2R,3R,5S)-2-(S-pantetheinyl)-carbapenam-3-carboxylate + AH2 + 2 S-adenosyl-L-methionine = (2R,3R,5S,6R)-6-(methyl)-2-(S-pantetheinyl)-carbapenam-3-carboxylate + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  • (2R,3R,5S,6R)-6-(methyl)-2-(S-pantetheinyl)-carbapenam-3-carboxylate + AH2 + 2 S-adenosyl-L-methionine = (2R,3R,5S,6R)-6-(ethyl)-2-(S-pantetheinyl)-carbapenam-3-carboxylate + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
Comment(s)
  • A radical SAM (AdoMet) enzyme that catalyzes two consecutive methylations during the biosynthesis of complex carbapenem antibiotics.
  • The enzyme adds a methyl group at position 6, followed by a second methylation that converts the methyl group to an ethyl group.
  • The enzyme binds a [4Fe-4S] cluster and requires a cobalamin cofactor and an electron donor.
  • Methyl viologen can be used in vitro.
Cross-references
BRENDA2.1.1.394
EC2PDB2.1.1.394
ExplorEnz2.1.1.394
KEGG Ligand Database for Enzyme Nomenclature2.1.1.394
IUBMB Enzyme Nomenclature2.1.1.394
MEDLINEFind literature relating to 2.1.1.394
MetaCyc2.1.1.394
Rhea expert-curated reactions2.1.1.394
UniProtKB/Swiss-Prot
Q83XP3, THNK_STREN

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