FastQCFastQC Report
Wed 8 May 2024
EGAF00001421073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256998
Total Bases25.6 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT32961.2825002529202563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.8202398462244843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.6533124771398999No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT11240.43735748916333983TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG9390.3653724931711531TruSeq Adapter, Index 10 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.32140327940295255No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT7870.3062280640316267RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT4010.15603234266414523No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA3900.15175215371325848RNA PCR Primer, Index 10 (96% over 28bp)
CATTTAAAGTACGCTTTTTAAAAAAATAATCATGTCTTGGCATTTTTTCA3660.1424135596385964No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT3330.12957299278593606TruSeq Adapter, Index 10 (96% over 26bp)
GTATACACCAGGGGCTCAGTCGCTGTTTGTCTTATTTTCTTGTTGACTCT2630.1023354267348384No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.1015572105619499Clontech SMART CDS Primer II A (100% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)