FastQCFastQC Report
Wed 8 May 2024
EGAF00001421251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625919
Total Bases62.5 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT46380.7409904476457816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.415548976784536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.3633057951587985No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG16380.26169520337296037RNA PCR Primer, Index 23 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT16190.2586596668258992RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT11510.18388960871933907RNA PCR Primer, Index 23 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.16679474500694177No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT7100.11343320781123437No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA6990.11167579191556735RNA PCR Primer, Index 23 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)