FastQCFastQC Report
Fri 31 May 2024
EGAF00001745370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7288604
Total Bases918.3 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN221620.30406371370978585No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT162040.22231966505520126No Hit
CACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG137560.18873298645392175No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG134210.184136770223763No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG133520.1831900868808348No Hit
GGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGG119080.16337833692158335No Hit
CACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCA118100.1620337721736563No Hit
GTCACCGATGCTTCCTCATTATCCACAGGGGACACCACCCCTCTTCCTGT114980.15775311705780695No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114340.1568750339571199No Hit
GGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGGTGACA104640.1435665869623319No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC99070.13592451997666494No Hit
GCCCTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACCAGC97060.1331667902385697No Hit
GCCCTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACCGAC94930.1302444199190956No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA92390.1267595276132439No Hit
GTCACCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGT91800.12595004475479804No Hit
GTCACCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGT89920.12337067564652984No Hit
GACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAG89570.12289047395084161No Hit
GGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGG89000.12210843118929222No Hit
CACCACGCCTCTTCCTGTCACCATCCCTTCCTCAGCATCCTCAGGTCACA78410.10757889988261125No Hit
CAGCATCCTCAGGTCACACCACCCCTCTTCCTGTCACCGACGCTTCCTCA76960.10558949285761718No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76560.10504069091968778No Hit
CACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACCA75750.10392936699538075No Hit
CACCACCCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG75350.10338056505745134No Hit
GCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGGTGACAC73380.10067771551314902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)