FastQCFastQC Report
Thu 9 May 2024
EGAF00002167567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10675720
Total Bases273.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8610868.065835372227822No Hit
TCAGTGCACTACAGAACTTTGT2630642.464133566635318No Hit
AAACCGTTACCATTACTGAGC1184451.109480203677129No Hit
TATTGCACTTGTCCCGGCCTGT1096431.0270314320720288No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC819150.7673018775314452No Hit
TTCAAGTAATCCAGGATAGGCT768450.7198109354685212No Hit
TCCTGTACTGAGCTGCCCCGA688510.6449307400343958No Hit
TCCTGTACTGAGCTGCCCCGAG687380.6438722634164253No Hit
AAGCTGCCAGTTGAAGAACTGT565390.5296036239241944No Hit
TGAGGTAGTAGGTTGTATAGTT479000.44868168142289233No Hit
TGAGGGGCAGAGAGCGAGACTTT467210.4376379298070762No Hit
TGAGGTAGTAGGTTGTATAGT450180.4216858441397864No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT422670.39591709036954886No Hit
TCAGTGCACTACAGAACTTTGC415060.388788765535252No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT381840.3576714263768626No Hit
CCCATAAAGTAGAAAGCACT372640.34905374063763384No Hit
TGAGGGGCAGAGAGCGAGACT365580.34244060353774736No Hit
CGCGACCTCAGATCAGA365020.34191604875362036No Hit
TAGCTTATCAGACTGATGTTGA354380.3319495078552079No Hit
TTCACAGTGGCTAAGTTCCG342320.3206528458970449No Hit
AAACCGTTACCATTACTGAG340790.3192196872904123No Hit
TAGCTTATCAGACTGATGTTGAC327300.3065835372227822No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC316780.29672940092096833No Hit
TGAGGTAGTAGATTGTATAGTT315290.29533371051320195No Hit
CATTGCACTTGTCTCGGTCTGA294420.2757846777547557No Hit
TGAGGTAGTAGATTGTATAGT286660.26851584717471044No Hit
TGGCTCAGTTCAGCAGGAAC263730.24703720217465427No Hit
TCATTGCACTACAGAACTTTGT260550.2440584803647904No Hit
AAACGGTTACCATTACTGAGT256210.23999318078780635No Hit
TAACCGTTACCATTACTGAGT249660.2338577632234641No Hit
TGGAGTGTGACAATGGTGTTT230880.21626644385577742No Hit
TGAGGTAGTAGGTTGTGTGGTT226880.2125196239691562No Hit
TATTGCACTTGTCCCGGCCTGC213480.19996777734897506No Hit
TATTTCACTTGTCCCGGCCTGT208810.19559336513134476No Hit
TGAGGTAGTAGTTTGTGCTGTT208520.19532172068956474No Hit
TAGCTTATCAGACTGATGTTG207500.1943662816184763No Hit
AAACCGTTACCATTACTGAGTT205420.19241793527743328No Hit
TGAGGTAGTAGGTTGTGTGGT201060.18833390160101615No Hit
TCAGGGCACTACAGAACTTTGT200110.1874440318779436No Hit
TCCTGTACTGAGCTGCCCCG192600.18040937754081224No Hit
AAAAGCTGGGTTGAGAGGGCGA184860.17315928106020018No Hit
TCGTACCGTGAGTAATAATGCG179580.16821347880986015No Hit
TGAGGTAGTAGTTTGTACAGT173100.16214363059353373No Hit
CGCGACCTCAGATCAGACGT167240.15665453945963365No Hit
TCCTGTACTGAGCTGCCCCGAGA165670.1551839126541348No Hit
TCCTGTACTGAGCTGCCCCGAGT163880.15350721075487178No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC152180.1425477625865047No Hit
TGAGATGAAGCACTGTAGCT151290.14171409516173147No Hit
TGTAAACATCCCCGACTGGAAG148760.13934423158344356No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT148650.13924119403656146No Hit
TTCAAGTAATCCAGGATAGGCC139350.1305298378001671No Hit
TGGCTCAGTTCAGCAGGAACAG137490.12878756655288823No Hit
TGAGGTAGTAGGTTGTGTGG134350.12584631294189058No Hit
AAACCGTTACCATTACTG133780.12531239110804704No Hit
TGAGGGGCAGAGAGCGAGACTT132520.12413214284376137No Hit
AAAGCGTTACCATTACTGAGT126420.11841824251666397No Hit
TCCTTTACTGAGCTGCCCCGAG125270.11734103179926038No Hit
GGCTTGTCCGATGGTAGTGGGTTATCAGAAC124260.11639495977788852No Hit
CGCGACCTCAGATC124010.1161607835349747No Hit
TCCTTTACTGAGCTGCCCCGA122370.11462458738145999No Hit
AGACCGTTACCATTACTGAGT118720.11120561423491812No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG116110.10876081425889776No Hit
TGTAAACATCCTTGACTGGAAGCT114750.10748689549744656No Hit
TGAGGTAGTAGTTTGTGCTGT112500.1053793093112221No Hit
AAACCGTTACCATTACTGAGA110000.10303754688208383No Hit
TAGCAGCACGTAAATATTGGCG109300.10238185340192513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)