FastQCFastQC Report
Fri 31 May 2024
EGAF00002300279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605243
Total Bases328.2 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGC77930.29912756698703347No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTT66700.2560221829595167No Hit
AGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCT43240.16597300136685908No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC40440.15522544346151204No Hit
CACTGACCTGGCAGCGGGATGGGGAGGACCAGACCCAGGACACGGAGCTT38360.14724154330325426No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT37070.14228998983971938No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT35790.13737682051156072No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC32100.12321307455772841No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT31150.11956658169698567No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCG30410.11672615567914395No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT30100.11553624748248052No Hit
CTGGGCTTCTACCCTGCGGAGATCACACTGACCTGGCAGCGGGATGGGGA28300.10862710311475743No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT28250.10843518243787624No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC28090.1078210362718564No Hit
GAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTT28060.1077058838657277No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC27310.10482707371250972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)