FastQCFastQC Report
Fri 31 May 2024
EGAF00002300454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6007541
Total Bases756.9 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA242980.4044583299556341No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG182090.30310238415351637No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG103450.17220023966544715No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC99980.16642416589416537No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG85990.14313676760591396No Hit
GGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC85880.14295366440278975No Hit
GCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGA75660.1259417122579771No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT68420.11389019234325658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)