FastQCFastQC Report
Sun 5 May 2024
EGAF00002540101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78332070
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5400370.6894200549021621TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2253370.28766889474515356TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)