FastQCFastQC Report
Fri 31 May 2024
EGAF00003249748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301644
Total Bases157.2 Mbp
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG85670.6581676710375495No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG75290.5784223643331049No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA71740.5511491621364981No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA57730.44351604586200216No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT57460.44144174597662644No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC56030.4304556391763032No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT53160.4084065996539761No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG47460.3646158242960441No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA45740.3514017657669839No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT41550.3192117045828199No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC40590.311836416101484No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG40220.30899385699930243No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA40210.30891703107762186No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC39810.30584399421039854No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT38660.2970090132171316No Hit
GGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTC34300.26351291136439764No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC34160.26243734846086947No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT33890.26036304857549375No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC33580.2579814450033957No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT33160.25475475629281125No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA31140.23923592011333358No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT30670.23562510179434623No Hit
GGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTT30200.23201428347535885No Hit
GGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCAT29490.22655964303603748No Hit
GGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAAC29240.22463899499402296No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA29180.22417803946393947No Hit
GGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGTC26850.20627759971236373No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG26790.20581664418228024No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC25980.19959374452615308No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC25900.1989791371527084No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC25890.1989023112310278No Hit
GTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAG25820.19836452977926378No Hit
GTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGC25510.1959829262071657No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC25180.1934476707917065No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG24480.18806985627406572No Hit
GGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGGGGCATGGTGGGTT24040.1846895157201201No Hit
GGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGAGTTGAATAAC23930.1838444305816337No Hit
GGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTAATTCTGTCCACGTC23670.18184695661793854No Hit
GAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATC23130.1776983568471871No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG22480.17270467193794925No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC22460.1725510200945881No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG22380.17193641272114343No Hit
GCTCCAATATGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT21950.16863289808887838No Hit
CTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTT21950.16863289808887838No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA21740.16701955373358612No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT21730.16694272781190556No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC21490.1650989056915716No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA21340.16394651686636286No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG21270.16340873541459877No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG21250.1632550835712376No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA21240.16317825764955704No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG21060.16179539105930654No Hit
GCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCA21020.16148808737258422No Hit
GGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTC20520.15764679128855508No Hit
GGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATC19260.1479667251568017No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC18950.14558512158470366No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACACTGTTGCT18590.1428193884042027No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT18560.14258891063916093No Hit
GCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTAT18500.14212795510907744No Hit
GCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATT18410.1414365218139522No Hit
GGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACA18400.14135969589227163No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC18370.14112921812722987No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG18270.14036095891042405No Hit
CTCCAGCAGCTCCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTT17710.13605870729631145No Hit
GGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCT17530.13467584070606095No Hit
AGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGT17400.13367710372421338No Hit
AGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAA17250.13252471489900466No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT17190.13206375936892115No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC16500.12676277077296097No Hit
GGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCA16150.12407386351414057No Hit
CCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCA15920.12230686731548718No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC15770.12115447849027844No Hit
GGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTT15640.12015574150843088No Hit
CTTGAGATTATCATCGGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG15490.11900335268322214No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG15210.11685222687616582No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT15100.11600714173767943No Hit
TGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGT14940.11477792699079012No Hit
GGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTT14630.11239632341869205No Hit
GGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGGGGCATGATGGGTC14500.11139758643684448No Hit
GGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCT14400.11062932722003865No Hit
GTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGTCCTGC14310.10993789392491342No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC14230.10932328655146877No Hit
GTGCAGGGTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCAT14060.10801724588289886No Hit
TCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC14000.10755629035281537No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTAAGACGGAGCACCAGGTGCAG13960.10724898666609303No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT13830.10625024968424547No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG13630.10471373125063381No Hit
GGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTAATTC13580.1043296016422309No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC13530.10394547203382799No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA13460.10340769058206391No Hit
GGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTT13320.10233212767853576No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG13310.10225530175685517No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG13180.10125656477500761No Hit
GTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTG13150.10102608700996586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)