FastQCFastQC Report
Fri 3 May 2024
EGAF00005432316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48995684
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG2876880.5871700862467805No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC1849850.3775536637063787No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1467590.29953454675722047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1369310.2794756370785639No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1360590.27769588847866683No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG1275100.2602474128129327No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG1236380.25234467591063736No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG1224230.24986486564816607No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT1177220.24027014297830804No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG1176660.24015584719666327No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA1121750.22894873760717374No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1107060.2259505143350994No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA1022140.20861837544711082No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA958470.19562335327332098No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA935730.1909821281401031No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC903880.184481555559057No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG899170.18352024639558048No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA874770.17854021590962993No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA819900.16734127030454357No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGAT789470.16113051916981097No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACAT786790.1605835322147967No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC774200.15801391812389026No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT764440.15602190592951004No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA749780.15302980564573812No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA740970.15123168808093385No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT737140.15044998657432765No Hit
GTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGAC729780.1489478134441393No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA718150.14657413497890956No Hit
GGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCT717090.14635778939222482No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG704540.14379633928572158No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC704370.14376164235200797No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT693420.14152675162163264No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG693330.14150838265672544No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG687460.14031031794555618No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC682890.13937758272749087No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG681080.13900816243324615No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC679860.13875916090894863No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA673430.1374468004161346No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC663080.13533436945180724No Hit
GGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA657580.13421182159636755No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC655780.13384444229822365No Hit
GGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTT649450.13255249176641762No Hit
ACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTC637300.13007268150394635No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA631330.1288542068317691No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT627020.12797453751232454No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG624380.1274357145417135No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT619990.12653971725346255No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC603080.12308839284701077No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG599200.12229648635990059No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG598760.12220668253146542No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC595800.12160254768562881No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG585210.11944113281488222No Hit
GTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGC574710.11729808690904284No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC569650.11626534288203835No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA566580.11563875707909294No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC566010.11552242030134735No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT564940.11530403371856184No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC561290.11455907014177004No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT560570.11441211842251249No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG559180.11412841996450136No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC558060.11389982840121184No Hit
GTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGT551020.11246296714624905No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT549040.11205884991829075No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG546670.11157513384240131No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA544980.1112302055013662No Hit
ATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGG541260.11047095495186883No Hit
AGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCT534520.10909532357993001No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA533280.10884224006343088No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT530900.10835648299144063No Hit
TGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCAC528430.10785235695454318No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCC525350.10722373015549695No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA523000.1067440960718091No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG519670.10606444437024289No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC511400.10437654059488179No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC510500.10419285094580985No Hit
GGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA506350.1033458375639781No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC504880.10304581113716056No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA497580.10155588398357701No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA492850.10059049282789888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)