FastQCFastQC Report
Sat 11 May 2024
EGAF00005475453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36316227
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length37-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1297030.35714888553813695TruSeq Adapter, Index 1 (97% over 36bp)
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAG913040.25141378260467423No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA848370.23360631598651477No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA805780.22187877611845527No Hit
GTCCGGTCTTCTCCTTGGGGGTCACCCGCACTCGATATCCAGTGAGCTGA772260.2126487423927601No Hit
CCCGGGGCCTGTGGAAGACTTCACAGCCTTGAGTTGGATGCCAGGCTCGG671910.18501646660596102No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT657080.18093289261574447No Hit
GCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTACTGGTTG516010.14208799829343505No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGTAGGATAGTTCAGACGGT510620.14060381327608729No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG468620.12903873521883208No Hit
GTCGGGTGTGTTATTATTCTGAATTTTGGGGGAGGTTATATGGGTTTAAT450730.12411256268444405No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT446890.12305518411920931No Hit
GGGGAGGGAGGTGTAGGGGAAGGAGATATGGAGAGGGAGGCAGAGCTACA423080.11649888629675104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)