FastQCFastQC Report
Fri 3 May 2024
EGAF00006144272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932802051
Total Bases439.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTAT1719002715.861298103681666TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTATG144184320.49162649743386994TruSeq Adapter, Index 22 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTATGCC74124240.2527420491087211TruSeq Adapter, Index 22 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTATGCCGT35903370.12242002486242806TruSeq Adapter, Index 22 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)