FastQCFastQC Report
Sat 4 May 2024
EGAF00006144286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035946687
Total Bases455.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTAT668466802.2018397189331145TruSeq Adapter, Index 11 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTATGCCGT245652330.8091457305620334TruSeq Adapter, Index 11 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTATG49447300.1628727546887914TruSeq Adapter, Index 11 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTATGCC34797950.11461976637799898TruSeq Adapter, Index 11 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)