FastQCFastQC Report
Wed 8 May 2024
EGAF00008072556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008072556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380829
Total Bases253.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC378681.1200803116632045No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC338191.000316786208353No Hit
GCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCC220270.6515265930338388No Hit
GGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGC194020.5738829145159368No Hit
CCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCG130940.38730145771939367No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC129760.38381118950411275No Hit
CAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG96300.2848413806199604No Hit
CCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGAC96210.2845751737221847No Hit
CTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCT80640.23852138040699483No Hit
GAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGC77650.22967739569200335No Hit
GGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGT63160.18681808515012147No Hit
TGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCT61800.18279540313928921No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGG58920.17427678241046796No Hit
GCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTG58250.17229501994924915No Hit
AAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAG56440.16694130344953856No Hit
AGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCC55710.16478206972313597No Hit
TGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCA50790.15022942597806632No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTC50480.14931249110795014No Hit
CAGAGGACCTGCAGGTGGGGCAGGTGGAGCTGGGCGGGGGCCCTGGTGCA49920.14765609263290158No Hit
TTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTA49800.14730115010253403No Hit
CCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCT49610.1467391577627854No Hit
GCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC48480.14339678226849095No Hit
CTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTG47630.1408826060117208No Hit
GCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCCT47000.13901915772729115No Hit
CAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCC45770.13538099679102375No Hit
CGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAA45260.13387249103696164No Hit
CAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTG44600.1319203071199401No Hit
AAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCC44190.13070758680785097No Hit
GGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCA43560.12884413852342133No Hit
CTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACC43010.12721731859257004No Hit
GGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGA41010.12130160975311086No Hit
AGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCC40080.11855080514276232No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCT40020.11837333387757853No Hit
GCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAG39650.11727892774227859No Hit
CGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCA39390.1165098855931489No Hit
CCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGG39200.11594789325340027No Hit
ACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCAT38880.1150013798390868No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCC38010.11242804649392205No Hit
TGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTC37710.11154069016800316No Hit
TGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCT37260.11020965567912486No Hit
GGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGAC37120.10979555606036272No Hit
GAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG36820.10890819973444384No Hit
CAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGA36580.10819831467370872No Hit
CTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGC35740.10571371696113586No Hit
CTCTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGG35670.10550666715175479No Hit
CAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGG34820.10299249089498463No Hit
CCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCG34670.1025488127320252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)