FastQCFastQC Report
Wed 8 May 2024
EGAF00008072746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008072746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9152821
Total Bases686.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC599380.6548582125663771No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC355270.3881535539698635No Hit
GCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCC337680.36893543531551637No Hit
GGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGC326600.35682987791414256No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC200310.21885055984379023No Hit
CCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCG161440.17638277859907892No Hit
CAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG159340.1740884040013456No Hit
CCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGAC139130.1520077798964931No Hit
CTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCT105230.11497001853308395No Hit
AGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCC92630.10120377094668409No Hit
AAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAG91710.10019861636101045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)