import pandas as pd
import matplotlib.pyplot as plt

df = pd.DataFrame({'Gene_set': ['2022', '2022', '2022', '2022', '2022', '2021', '2021', '2021', '2021', '2021'], 
                                  'Term': ['regulation of cation channel activity (GO:2001257)', 
                                                'establishment of mitochondrion localization, microtubule-mediated (GO:0034643)', 
                                                'DNA-templated transcription, termination (GO:0006353)', 
                                                'mitochondrion transport along microtubule (GO:0047497)',
                                                 'mitochondrial fission (GO:0000266)', 'regulation of cation channel activity (GO:2001257)', 
                                                'establishment of mitochondrion localization, microtubule-mediated (GO:0034643)', 
                                                'DNA-templated transcription, termination (GO:0006353)', 
                                                'mitochondrion transport along microtubule (GO:0047497)', 
                                                'mitochondrial fission (GO:0000266)'], 
                                  'area': [82.83045070066913, 625.0, 66.30612244897958, 260.94674556213016, 177.7777777777778, 82.83045070066913, 625.0, 66.30612244897958, 260.94674556213016, 177.7777777777778], 
                                  'p_inv': [2.5549417930429006, 0.7132534017074245, 0.6939727070003071, 0.6939727070003071, 0.6489820335167326, 2.5549417930429006, 0.7132534017074245, 0.6939727070003071, 0.6939727070003071, 0.6489820335167326]})

## scatters
fig, (ax1, ax2) = plt.subplots(2,1, figsize=(4,8))
smax = df["area"].max()
x = "Gene_set"
y = "Term"
blk_sc1 = ax1.scatter( x=x, y=y, s=smax * 1.2, edgecolors="none", c="black", data=df, marker='o', zorder=0)
wht_sc1 = ax1.scatter( x=x, y=y, s=smax * 1.0, edgecolors="none", c="white", data=df, marker='o', zorder=1)
sc2 = ax1.scatter(x=x, y=y, data=df, s="area", edgecolors="none", c="p_inv", cmap=plt.cm.RdBu, marker="o", zorder=2)
ax1.set_title("set edgecolors='none', scatter aligned at center", fontsize=16)
ax1.margins(x = 0.25, y = 0.1)

## when set edgecolors, could not align at center
blk_sc2 = ax2.scatter( x=x, y=y, s=smax * 1.2, edgecolors="gray", c="white", data=df, marker='o', zorder=0)
sc2 = ax2.scatter(x=x, y=y, data=df, s="area", edgecolors="none", c="p_inv", cmap=plt.cm.RdBu, marker="o", zorder=2)
ax2.set_title("set edgecolors='gray', scatter not aligned at center", fontsize=16)
ax2.margins(x = 0.25, y = 0.1) 
plt.show()