diff --git a/src/runtime/Python.Runtime.csproj b/src/runtime/Python.Runtime.csproj
index 82825a626..1fea78082 100644
--- a/src/runtime/Python.Runtime.csproj
+++ b/src/runtime/Python.Runtime.csproj
@@ -145,7 +145,6 @@
   </ItemGroup>
   <ItemGroup Condition=" '$(PythonInteropFile)' == '' ">
     <Compile Include="interop27.cs" />
-    <Compile Include="interop33.cs" />
     <Compile Include="interop34.cs" />
     <Compile Include="interop35.cs" />
     <Compile Include="interop36.cs" />