Many stem cells (SCs) respond to Wnt signaling, but whether b-catenin's DNA binding partners, the... more Many stem cells (SCs) respond to Wnt signaling, but whether b-catenin's DNA binding partners, the Tcfs, play a role in SCs in the absence of Wnts, is unknown. In adult skin, quiescent multipotent progenitors express Tcf3 and commit to a hair cell fate in response to Wnt signaling. We find that embryonic skin progenitors also express Tcf3. Using an inducible system in mice, we show that upon Tcf3 reactivation, committed epidermal cells induce genes associated with an undifferentiated, Wnt-inhibited state and Tcf3 promotes a transcriptional program shared by embryonic and postnatal SCs. Further, Tcf3-repressed genes include transcriptional regulators of the epidermal, sebaceous gland and hair follicle differentiation programs, and correspondingly, all three terminal differentiation pathways are suppressed when Tcf3 is induced postnatally. These data suggest that in the absence of Wnt signals, Tcf3 may function in skin SCs to maintain an undifferentiated state and, through Wnt signaling, directs these cells along the hair lineage.
A total 67 strains were isolated from five different stations of Aksu River. Total aerobic bacter... more A total 67 strains were isolated from five different stations of Aksu River. Total aerobic bacteria number in the river was determined as 2 x 10 7 CFU/mL and fecal coliforms were determined >1100 MPN/100 mL. Strains of Enterobacteriaceae (66 isolates), representative of the human and animal commensal flora, and Pseudomonas sp. (1 isolate) were selected for antibiotic susceptibility testing. Among the isolates, 67,2% were Escherichia coli, 29,8% Klebsiella spp., 1,5% Citrobacter spp., and 1,5% Pseudomonas spp. Eleven antibiotics (Meropenem, Cefoxitin, Ceftazidime, Ceftriaxone, Imipenem, Sulbactam/Cefoperazone, Cefazoline, Cefotaxime, Aztreonam, Ampicillin/Sulbactam, Penicillin G) were used for determination of antibiotic resistance of isolates. Multiple antibiotic resistances were determined in 27 isolates (40%) and they were resistant to five or over antibiotics and 49,3% of the isolates were found to be β-lactamase producing.
Providing efficient data aggregation while preserving data privacy is a challenging problem in wi... more Providing efficient data aggregation while preserving data privacy is a challenging problem in wireless sensor networks research. In this paper, we present two privacy-preserving data aggregation schemes for additive aggregation functions. The first scheme -Cluster-based Private Data Aggregation (CPDA)leverages clustering protocol and algebraic properties of polynomials. It has the advantage of incurring less communication overhead. The second scheme -Slice-Mix-AggRegaTe (SMART)builds on slicing techniques and the associative property of addition. It has the advantage of incurring less computation overhead. The goal of our work is to bridge the gap between collaborative data collection by wireless sensor networks and data privacy. We assess the two schemes by privacy-preservation efficacy, communication overhead, and data aggregation accuracy. We present simulation results of our schemes and compare their performance to a typical data aggregation scheme -TAG, where no data privacy protection is provided. Results show the efficacy and efficiency of our schemes. To the best of our knowledge, this paper is among the first on privacy-preserving data aggregation in wireless sensor networks.
BRCA1 mRNA and protein levels are regulated by the steroid hor monesestrogenand progesteronein hu... more BRCA1 mRNA and protein levels are regulated by the steroid hor monesestrogenand progesteronein humanbreastcancercells. BRCA1 mRNA and protein levels were significantly decreased in estrogen-de pleted MCF-7 and BT2OT cells and increased again after stimulation with @3-estradiol. The increase in BRCA1 expression upon stimulation with estrogen was not coordinated with the early induction of the estrogen dependentpS2 gene but closely paralleledthe delayed increasein the S-phase dependent marker cydlin A. T47-D cells deprived of steroid hormones and subsequently stimulated with progesterone also showed a delayed increase in BRCA1 mRNA expression. However, no change in BRCA1 protein was detected in these cells. When considered together, the data suggest that steroid hormones may affect BRCA1 expression mdi racily by altering the proliferative status of the cells rather than acting directly on DNA sequences In the BRCA1 gene Itself.
Many stem cells (SCs) respond to Wnt signaling, but whether b-catenin's DNA binding partners, the... more Many stem cells (SCs) respond to Wnt signaling, but whether b-catenin's DNA binding partners, the Tcfs, play a role in SCs in the absence of Wnts, is unknown. In adult skin, quiescent multipotent progenitors express Tcf3 and commit to a hair cell fate in response to Wnt signaling. We find that embryonic skin progenitors also express Tcf3. Using an inducible system in mice, we show that upon Tcf3 reactivation, committed epidermal cells induce genes associated with an undifferentiated, Wnt-inhibited state and Tcf3 promotes a transcriptional program shared by embryonic and postnatal SCs. Further, Tcf3-repressed genes include transcriptional regulators of the epidermal, sebaceous gland and hair follicle differentiation programs, and correspondingly, all three terminal differentiation pathways are suppressed when Tcf3 is induced postnatally. These data suggest that in the absence of Wnt signals, Tcf3 may function in skin SCs to maintain an undifferentiated state and, through Wnt signaling, directs these cells along the hair lineage.
A total 67 strains were isolated from five different stations of Aksu River. Total aerobic bacter... more A total 67 strains were isolated from five different stations of Aksu River. Total aerobic bacteria number in the river was determined as 2 x 10 7 CFU/mL and fecal coliforms were determined >1100 MPN/100 mL. Strains of Enterobacteriaceae (66 isolates), representative of the human and animal commensal flora, and Pseudomonas sp. (1 isolate) were selected for antibiotic susceptibility testing. Among the isolates, 67,2% were Escherichia coli, 29,8% Klebsiella spp., 1,5% Citrobacter spp., and 1,5% Pseudomonas spp. Eleven antibiotics (Meropenem, Cefoxitin, Ceftazidime, Ceftriaxone, Imipenem, Sulbactam/Cefoperazone, Cefazoline, Cefotaxime, Aztreonam, Ampicillin/Sulbactam, Penicillin G) were used for determination of antibiotic resistance of isolates. Multiple antibiotic resistances were determined in 27 isolates (40%) and they were resistant to five or over antibiotics and 49,3% of the isolates were found to be β-lactamase producing.
Providing efficient data aggregation while preserving data privacy is a challenging problem in wi... more Providing efficient data aggregation while preserving data privacy is a challenging problem in wireless sensor networks research. In this paper, we present two privacy-preserving data aggregation schemes for additive aggregation functions. The first scheme -Cluster-based Private Data Aggregation (CPDA)leverages clustering protocol and algebraic properties of polynomials. It has the advantage of incurring less communication overhead. The second scheme -Slice-Mix-AggRegaTe (SMART)builds on slicing techniques and the associative property of addition. It has the advantage of incurring less computation overhead. The goal of our work is to bridge the gap between collaborative data collection by wireless sensor networks and data privacy. We assess the two schemes by privacy-preservation efficacy, communication overhead, and data aggregation accuracy. We present simulation results of our schemes and compare their performance to a typical data aggregation scheme -TAG, where no data privacy protection is provided. Results show the efficacy and efficiency of our schemes. To the best of our knowledge, this paper is among the first on privacy-preserving data aggregation in wireless sensor networks.
BRCA1 mRNA and protein levels are regulated by the steroid hor monesestrogenand progesteronein hu... more BRCA1 mRNA and protein levels are regulated by the steroid hor monesestrogenand progesteronein humanbreastcancercells. BRCA1 mRNA and protein levels were significantly decreased in estrogen-de pleted MCF-7 and BT2OT cells and increased again after stimulation with @3-estradiol. The increase in BRCA1 expression upon stimulation with estrogen was not coordinated with the early induction of the estrogen dependentpS2 gene but closely paralleledthe delayed increasein the S-phase dependent marker cydlin A. T47-D cells deprived of steroid hormones and subsequently stimulated with progesterone also showed a delayed increase in BRCA1 mRNA expression. However, no change in BRCA1 protein was detected in these cells. When considered together, the data suggest that steroid hormones may affect BRCA1 expression mdi racily by altering the proliferative status of the cells rather than acting directly on DNA sequences In the BRCA1 gene Itself.
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Papers by Hoang Nguyen