Papers by Sandeep Singh Rana
Journal of perioperative echocardiography, 2016
Introduction: Incidence of right ventricular (RV) dysfunction in early postoperative period after... more Introduction: Incidence of right ventricular (RV) dysfunction in early postoperative period after Tetralogy of Fallot (TOF) repair ranges from 28 to 63%. Echocardiography is the first-line tool for the assessment of RV function in early postoperative period. As speckle tracking echocardiography (STE) has emerged as a new promising tool for assessing myocardial performance and is independent of geometric assumptions and angle dependence, it is more sensitive for detecting changes in myocardial performance than conventional echocardiographic parameters of RV function. The current study demonstrates echocardiographic parameters assessed by conventional twodimensional (2D) echocardiography and STE in patients before and after TOF repair. Materials and methods: Fifty-nine consecutive patients planned for complete intracardiac repair for TOF were enrolled in this prospective cohort study. The 2D echocardiography and STE were performed a day prior to TOF repair, in the early postoperative period between days 3 and 7 and after discharge at 3 months. The median age of patients was 6 years, with 57.6% males (34/59). Baseline hemoglobin and room air oxygen saturation were 17.7 ± 3.7 gm% and 79.4% ± 8% respectively. Two patients did not survive the procedure (3.4%). Right ventricular longitudinal peak systolic strain (RV LPSS) in early postoperative period was significantly decreased in all segments of both septal and lateral wall. However, RV LPSS assessed at midterm follow-up at 3 months postoperatively significantly improved in all segments of RV compared with assessment done in the early postoperative period, and was significantly better than preoperative values in all three segments of the septal wall. Our study shows that the use of 2D strain or speckle tracking is a feasible and easy-to-implement technique for the evaluation of RV function after TOF repair.
International journal of research in engineering and innovation, 2022
In the field of satellite image processing, this work focuses on the Image Analysis of Remote Sen... more In the field of satellite image processing, this work focuses on the Image Analysis of Remote Sensitivity which includes Spectral, Temporary, and Spatial Properties. We used multi-spectral remote sensor data to determine the spectral signature of the various objects in Meerut to separate the earth cover, how land use varied over time, and to perform interim analyzes to assess the climate impact on the area. Some combinations of remote sensor bands are useful for splitting the earth cover. The spatial distribution of land cover areas such as roads, urban areas, agricultural land, and water resources can be easily assessed by calculating their General Plant Diversity Index (NDVI). We conducted a groundbreaking survey to determine the NDVI boundary values, and based on that, we calculated the False Color Combination (FCC) of items in the category. Separated information may be used for municipal planning and management. The aim of the longterm thesis is to develop land use patterns to grow the city's sustainable economy and environment.
Journal of Medicinal Chemistry, Aug 28, 2014
Adenosine 5′-monophosphate activated protein kinase (AMPK) is a master sensor of cellular energy ... more Adenosine 5′-monophosphate activated protein kinase (AMPK) is a master sensor of cellular energy status that plays a key role in the regulation of whole-body energy homeostasis. AMPK is a serine/threonine kinase that is activated by upstream kinases LKB1, CaMKKβ and Tak1 among others. AMPK exists as αβγ trimeric complexes that are allosterically regulated by AMP, ADP and ATP. Dysregulation of AMPK has been implicated in a number of metabolic diseases including type 2 diabetes mellitus and obesity. Recent studies have associated roles of AMPK with the development of cancer and neurological conditions making it a potential therapeutic target to treat human diseases. This perspective focuses on the structure and function of AMPK, its role in human diseases and its direct substrates and provides a brief synopsis of key AMPK modulators and their relevance in human diseases.
Separations
Herbal nutraceuticals are foods derived from plants and/or their derivatives, such as oils, roots... more Herbal nutraceuticals are foods derived from plants and/or their derivatives, such as oils, roots, seeds, berries, or flowers, that support wellness and combat acute and chronic ailments induced by unhealthful dietary habits. The current review enlists various traditional as well as unexplored herbs including angelica, burnet, caraway, laurel, parsley, yarrow, and zedoary, which are rich sources of bioactive components, such as aloesin, angelicin, trans-anethole, and cholesteric-7-en-3β-ol. The review further compares some of the extraction and purification techniques, namely, Soxhlet extraction, ultrasound assisted extraction, microwave assisted extraction, supercritical fluid extraction, accelerated solvent extraction, hydro-distillation extraction, ultra-high-pressure extraction, enzyme assisted extraction, pulsed electric field extraction, bio affinity chromatography, cell membrane chromatography, and ligand fishing. Herbal nutraceuticals can be purchased in varied formulations,...
International Journal for Research in Applied Science and Engineering Technology
Pavement engineers much concerned about the tensile properties of bituminous mix because of the p... more Pavement engineers much concerned about the tensile properties of bituminous mix because of the problems associated with cracking. Hence tensile strength of bituminous concrete mix is important in pavement applications. The Indirect Tensile Strength (ITS) test is used to assess the tensile properties of the bituminous mix. Addition of cement or lime is known to impart Anti-Stripping properties to bituminous mixes. For adverse conditions however use of Anti-Stripping chemicals is desired. It is important to ensure that addition of such compound may not have adverse effect on other properties. Many researches were done on ITS individually; however, a combination has not been tried yet. In this proposal, an effort has been made to study the techniques of addition of Anti-Stripping and Cement material both individually and combined in suitable proportions on bituminous concrete.
Indian Journal of Medical and Paediatric Oncology, 2020
Objective Indian data on cancer patients with coronavirus disease 2019 (COVID-19) infection and i... more Objective Indian data on cancer patients with coronavirus disease 2019 (COVID-19) infection and its outcome are limited. Infectivity and outcome among cancer patients staying in large congregations are not known. We conducted this study to address this lacuna in literature. Methods This was a retrospective-prospective, observational study of hospitalized cancer patients with proven COVID-19 infection, conducted at tertiary care hospital in New Delhi after ethical approval. We analyzed clinical, laboratorial parameters, and outcomes in these patients. Results All 32 admitted male patients became COVID-19 positive and 10 (31%) patients were symptomatic. Median age was 37.5 years (range: 16-64). Sixteen health-care workers (HCWs) were working in the cancer ward (paramedical staff: 4, nurses: 6, residents: 4, and consultants: 2). Among HCWs, two nursing staff and three paramedical staff contracted asymptomatic COVID-19. Eighteen (56%) and 14 (44%) patients were on curative and palliative treatment, respectively. Leukopenia (total leukocyte count <4,000/μL) was seen in 37.5% of cases. Grades 4, 3, 2, and 1 neutropenia were present in 12.5% of patents. Two (6.25%) patients had features of critical COVID-19. Eight (25%) patients received treatment with hydroxychloroquine + azithromycin. Five patients died. Three patients had features of progressive disease with poor performance status. One critical COVID patient survived and one succumbed to COVID-19. Patients shared a common dining room, sanitation area. Conclusion Seventy percent of cancer patients were asymptomatic. Cancer patients living in congregation areas are susceptible to COVID-19 with 3% mortality rate. Recent chemotherapy and associated cytopenias may not increase the risk in cancer patients with COVID-19 treated with curative intent. Palliative intended patients are at increased risk of death. N-95, personal protective equipment, and adherence to infection control measures should be encouraged.
Canadian Journal of Anesthesia/Journal canadien d'anesthésie, 2021
Purpose There is some evidence for the use of intrathecal morphine as a means to provide prolonge... more Purpose There is some evidence for the use of intrathecal morphine as a means to provide prolonged analgesia in selective cardiac surgical patients; however, the hemodynamic effects of intrathecal morphine are not well defined. This study was designed to study the effect of intrathecal morphine on hemodynamic parameters in cardiac surgery patients. Methods In a prospective, double-blind study, 100 adult cardiac surgical patients were randomized to receive either intrathecal 40 mg of 0.5% hyperbaric bupivacaine alone (intrathecal bupivacaine [ITB] group, n = 50) or intrathecal 250 lg of morphine added to 40 mg of 0.5% bupivacaine (intrathecal bupivacaine and morphine [ITBM] group, n = 50). Hemodynamic data, pain scores, rescue analgesic use, spirometry, and vasopressor use were recorded every four hours after surgery for 48 hr. The primary outcome was the incidence of vasoplegia in each group, which was defined as a cardiac index [ 2.2 LÁmin -1 Ám -2 with the requirement of vasopressors to maintain the mean arterial pressure [ 60 mmHg with the hemodynamic episode lasting[four hours. Results Eighty-seven patients were analyzed (ITB group, n = 42, and ITBM group, n =45). The incidence of vasoplegia was higher in the ITBM group than in the ITB group [14 (31%) vs 5 (12%), respectively; relative risk, 2.6; 95% confidence interval [CI], 1.0 to 6.6; P = 0.04]. The mean (standard deviation [SD]) duration of vasoplegia was significantly longer in the ITBM group than in the ITB group [8.9 (3.0) hr vs 4.3 (0.4) hr, respectively; difference in means, 4.6; 95% CI, 3.7 to 5.5; P \ 0.001]. Conclusion Intrathecal morphine added to bupivacaine for high spinal anesthesia increases the incidence and duration of vasoplegia in cardiac surgery patients. Trial registration www.clinicaltrials.gov (NCT02825056); registered 19 June 2016.
Petroleum, 2021
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
International Journal of Applied Dental Sciences, 2017
The introduction of flowable composites provide expanded options for restorative dentistry. There... more The introduction of flowable composites provide expanded options for restorative dentistry. There is a growing trend among practitioners to use bulk-fill resin based composite materials because of their more simplified procedures. The main advancement of bulk-fill composite materials, namely increased depth of cure, which probably results from higher translucency, and low polymerization shrinkage stress are related to modifications in the filler content and/or organic matrix with the help of advanced technology. This article has enlighten different aspects of flowable bulkfill composite resin materials.
Onion (Allium cepa L.) is highly valued for their flavour and for their nutritional value in supp... more Onion (Allium cepa L.) is highly valued for their flavour and for their nutritional value in supplying minor constituents such as minerals and trace elements. The pungency in onion is mainly due to the presence of volatile componentallyl propyl disulphide in the bulb.Curing removes excess moisture from the outer layers of the bulb prior to storage. In wet weather, the bulbs can take longer time to dry and may develop higher level of rots during storage. The curing practice is completely dependent on climatic condition and any change in environment causes a drastic change in production of onion and this also influence price of onion in market. Thus, there was an urgent need to develop an onfarm curing method, which helps to achieve a stable onion production and minimizes the post-harvest losses. So, a modular ventilated structure was developed with the capacity of 150 kg.Curing studies were conducted with fresh Bellary big onion Variety to evaluate the performance of modular ventilat...
Journal of Environmental Biology, 2021
Present review is the description of a journey that originates from Virchows' cell theory and... more Present review is the description of a journey that originates from Virchows' cell theory and terminates with the role of molecular switches in cell death recently proposed by Orrenius. Landmark discoveries made, in between, to characterize regulated as well as accidental cell death have also been documented. It embraces the studies that were made in early nineties to understand cellular homeostasis in health and disease. Furthermore, the effects of foreign chemicals on different cell types witnessed in late nineties have been classified into necrosis, apoptosis, autophagy etc. Since it is important to know how a cell dies, studies made in our own and other laboratories on the role of reactive oxygen species, oxidative stress, intracellular Ca2+ homeostasis, redox imbalance, mitochondrial and ER stress in cell death have also been reviewed. Possibility of a cross talk amongst these mechanisms has also been examined. It discusses the impact of wonder molecules like CYP450, GSH, m...
SN Applied Sciences, 2021
The edible flowers and its several products gaining its importance as functional food. Pumpkin fl... more The edible flowers and its several products gaining its importance as functional food. Pumpkin flower mainly consumed in India and Mexico but due to lack of scientific research there is a neophobia among people. The objective of the paper is to analyse the physicochemical, biochemical properties, proximate analysis, antioxidant activities, anthocyanin content and fatty acid profiling. The fresh pumpkin flower was having an average moisture content of 85% (wb) with a dimension of 90 × 51 x 22 mm (l x w x t). The (L, a*, b*) value signifies the bright yellow color having gumminess (26 g) and chewiness (4.70 mJ). In this study the nutritional properties of the pumpkin flower were also determined and significant amount of Sodium (11.5 mg/100 g), Potassium (18.2 mg/100 g), Calcium (17.6 mg/100 g), phenol (17.39 µg/ml), flavonoid (17.13 µg/ml), antioxidant (51.65%DPPH) and anthocyanin (10.3 mg/100 g) was present. Among several fatty acids’ oleic acid (21%), myristic acid (15.99%) and stea...
Molecular and Cellular Biology, 2020
Epidermal growth factor receptor (EGFR) is a prototype receptor tyrosine kinase and an oncoprotei... more Epidermal growth factor receptor (EGFR) is a prototype receptor tyrosine kinase and an oncoprotein in many solid tumors. Cell surface display of EGFR is essential for cellular responses to its ligands. While postactivation endocytic trafficking of EGFR has been well elucidated, little is known about mechanisms of basal/preactivation surface display of EGFR. Here, we identify a novel role of the endocytic regulator EHD1 and a potential EHD1 partner, RUSC2, in cell surface display of EGFR. EHD1 and RUSC2 colocalize with EGFR in vesicular/tubular structures and at the Golgi compartment.
Journal of Perioperative Echocardiography, 2018
Subaortic membrane (SAM), a rare lesion in the adult population is suspected when there is a high... more Subaortic membrane (SAM), a rare lesion in the adult population is suspected when there is a high-velocity gradient across the aortic valve with normal functioning aortic valve. With rheumatic heart disease forming the leading cause of valvular pathology in the Indian population, SAM can be missed in adult patients with poor transthoracic echocardiography window and can be misinterpreted as rheumatic heart disease. We report a case of SAM causing aortic valve pathology leading to aortic stenosis and regurgitation which was diagnosed accurately using 2D and 3D transesophageal echocardiography during the intraoperative period.
Monthly Notices of the Royal Astronomical Society, 2019
We study the angular clustering of point sources in The GMRT (Giant Metrewave Radio Telescope) Sk... more We study the angular clustering of point sources in The GMRT (Giant Metrewave Radio Telescope) Sky Survey (TGSS). The survey at 150 MHz with δ > −53.5° has a sky coverage of 3.6π steradians, i.e. $90{{\ \rm per\ cent}}$ of the whole sky. We created subsamples by applying different total flux thresholds limit (Sflux ≫ 5σ) for good completeness and measured the angular correlation function ω(θ) of point sources at large scales (≥1°). We find that the amplitude of angular clustering is higher for brighter subsamples; this indicates that higher threshold flux samples are hosted by massive haloes and cluster strongly: this conclusion is based on the assumption that the redshift distribution of sources does not change with flux and this is supported by models of radio sources. We compare our results with other low-frequency studies of clustering of point sources and verify that the amplitude of clustering varies with the flux limit. We quantify this variation as a power-law dependence ...
Journal of medicinal chemistry, Jan 7, 2017
Heme-regulated inhibitor (HRI), an eukaryotic translation initiation factor 2 alpha (eIF2 kinas... more Heme-regulated inhibitor (HRI), an eukaryotic translation initiation factor 2 alpha (eIF2 kinase, plays critical roles in cell proliferation, differentiation, adaptation to stress, and hemoglobin disorders. HRI phosphorylates eIF2 that couples cellular signals including the endoplasmic reticulum (ER) stress to translation. We previously identified 1,3-diarylureas and 1-((1,4-trans)-4-aryloxycyclohexyl)-3-arylureas (cHAUs) as specific activators of HRI that trigger the eIF2 phosphorylation arm of ER-stress response as molecular probes for studying HRI biology and its potential as a druggable target. To develop drug-like cHAUs needed for in vivo studies we undertook bioassay guided structure-activity relationship studies and tested them in the surrogate eIF2 phosphorylation and cell proliferation assays. We further evaluated some of these cHAUs in endogenous eIF2 phosphorylation and expression of the transcription factor CHOP protein and mRNA demonstrating significantly improved...
In the Midsouth, soybean is often grown in close proximity to rice or in rotation with rice. Herb... more In the Midsouth, soybean is often grown in close proximity to rice or in rotation with rice. Herbicides used in rice can injure soybean via drift or carryover. Consequently, field trials were conducted to determine the response of soybean (cv. AG 4703) to imazosulfuron drift and carryover (at Fayetteville, Marianna, Keiser, and Pine Tree, AR) from rice. To assess the potential for carryover, soybean was planted into rice fields treated the previous year with imazosulfuron (rotation study). To evaluate in-season sensitivity of soybean to imazosulfuron (tolerance study) relative to halosulfuron, a common sulfonylurea herbicide applied to rice, both imazosulfuron and halosulfuron were applied preemergence (PRE) at varying rates and soybean was immediately seeded into treated plots. For the drift study, imazosulfuron was applied at the VC, V2, V6, and R2 growth stages of soybean at 1/256 to 1/4 times (X) the labeled rate of imazosulfuron (336 g ai ha -1 ). To evaluate carryover potential, imazosulfuron was applied PRE to rice at 112 to 672 g ha -1 for the rotation study; whereas for the tolerance study, imazosulfuron and halosulfuron were applied at 1/256 to 1/4X the labeled rate of imazosulfuron and halosulfuron (52 g ha -1 ). Soybean was highly sensitive to imazosulfuron drift, with injury (stunting and purple veins, typical of acetolactate synthase-inhibiting herbicides) resulting at all rates and application timings. At 2 weeks after treatment (2 WAT), topical application of the highest (1/4X) rate of imazosulfuron caused more than 73% injury at the VC application timing. Soybean recovered from the injury, with little to no injury observed from the lowest four rates of imazosulfuron applied at the VC and V2 growth stages by the end of the growing season. For the carryover trial at 2 weeks after planting (2 WAP), soybean exhibited 3 and 13% injury at Keiser (silty clay soil with 1.0% organic matter content and a pH of 8.3) and Pine Tree (silt loam soil with 2.3% organic matter content and a pH of 7.1), respectively, when imazosulfuron was applied to rice the previous year at 672 g ha -1 , a 2X rate. Injury to soybean was transient and not apparent by the end of the growing season. For the tolerance study, sulfonylurea-tolerant (STS) soybean was not injured. Moreover, no injury was observed on non-STS soybean by PREapplied imazosulfuron or halosulfuron regardless of herbicide rate, and yield was comparable to the non-treated control. Results of this research indicate that imazosulfuron should be applied with extreme caution to rice as off-target movement will likely cause injury to non-STS soybean (cv. AG 4703) in adjacent fields and applications of imazosulfuron may carryover to non-STS (cv. AG 4703) soybean on silt loam soils having low organic matter and a high soil pH, especially in fields where overlap of an imazosulfuron spray occurred in the preceding rice crop. Under conditions conducive for imazosulfuron to injure soybean via off-target movement or carryover, planting of STS soybean is recommended to avoid possible risks of soybean injury. Firstly, I would like to extend my love and sincere thanks to my parents, Dr. Raninder Singh Rana and Smt. Sneh Lata Rana; my brother, Randeep Singh Rana; and my beloved wife, Trisha Sanwal Rana for their faith, love, and support that have always been a driving force behind all my personal as well as professional ventures. Many thanks to all my dear friends back in India and here in the USA;
World journal of cardiology, Jan 26, 2015
Constrictive pericarditis (CP) is an inflammatory disease of pericardium. Pericardial calcificati... more Constrictive pericarditis (CP) is an inflammatory disease of pericardium. Pericardial calcification in X-ray provides a clue for the diagnosis of CP. An extensive "eggshell" type of calcification is rarely seen in CP. We hereby report a case of CP with eggshell calcification of pericardium, encircling whole of the heart. A need for multimodality imaging and hemodynamic assessment followed by surgical pericardiectomy is discussed.
Xylanase catalyze the hydrolysis of xylan, the major constituent of hemicellulose. Applications o... more Xylanase catalyze the hydrolysis of xylan, the major constituent of hemicellulose. Applications of xylanases can be found in the food, feed and paper industry. The major problem in the field of paper bleaching is the removal of lignin and its derivatives, which are linked to cellulose and xylan. Xylanases are more suitable in paper and pulp industry than lignin degrading systems. This study aimed to find out the most efficient producer of xylanase enzyme isolated from soil samples of Jharna village. Various Aspergillus species were isolated from soil samples. Comparative studies were done to identify the most efficient producer of Xylanase among various species of Aspergillus namely A.niger, A. fumigatus and A. flavus after three days of incubation, at optimum temperature and pH conditions. Wheat substrate provide maximum yield at optimum temperature and pH conditions as compared to rice substrate. Aspergillus fumigatus showed the highest enzyme production in presence of wheat substrate as a carbon source. A. fumigatus showed on wheat substrate as the source of xylan produced highest enzymatic activity, clearing zones formation i.e. 79 mm at 37°C and pH 9 and produced high amount of Xylanase with optical density reading 0.897 at pH 7.4 respectively, thus A. fumigatus produced maximum amount of cellulose free Xylanase.
Journal of Medicinal Chemistry, 2015
Adenosine 5′-monophosphate activated protein kinase (AMPK) is a master sensor of cellular energy ... more Adenosine 5′-monophosphate activated protein kinase (AMPK) is a master sensor of cellular energy status that plays a key role in the regulation of whole-body energy homeostasis. AMPK is a serine/threonine kinase that is activated by upstream kinases LKB1, CaMKKβ and Tak1 among others. AMPK exists as αβγ trimeric complexes that are allosterically regulated by AMP, ADP and ATP. Dysregulation of AMPK has been implicated in a number of metabolic diseases including type 2 diabetes mellitus and obesity. Recent studies have associated roles of AMPK with the development of cancer and neurological conditions making it a potential therapeutic target to treat human diseases. This perspective focuses on the structure and function of AMPK, its role in human diseases and its direct substrates and provides a brief synopsis of key AMPK modulators and their relevance in human diseases.
Uploads
Papers by Sandeep Singh Rana