This paper proposes a new method to extract pores on high resolution fingerprints. The basic idea... more This paper proposes a new method to extract pores on high resolution fingerprints. The basic idea of this method is to binarize the fingerprint images based on multi-scale morphological transformation, and then extract pores by different strategies. The closed pores are extracted by the size of connected regions, and the open pores are detected using the skeleton of valleys. The noise and false detected points are finally removed by using a comprehensive selection rule. Experimental results have shown that the proposed method can improve the accuracy of existing methods.
An effective and stable quantitative resistance locus, QSc.VR4, was fine mapped, characterized an... more An effective and stable quantitative resistance locus, QSc.VR4, was fine mapped, characterized and physically anchored to the short arm of 4H, conferring adult plant resistance to the fungus Rhynchosporium commune in barley. Scald caused by Rhynchosporium commune is one of the most destructive barley diseases worldwide. Accumulation of adult plant resistance (APR) governed by multiple resistance alleles is predicted to be effective and long-lasting against a broad spectrum of pathotypes. However, the molecular mechanisms that control APR remain poorly understood. Here, quantitative trait loci (QTL) analysis of APR and fine mapping were performed on five barley populations derived from a common parent Vlamingh, which expresses APR to scald. Two QTLs, designated QSc.VR4 and QSc.BR7, were detected from a cross between Vlamingh and Buloke. Our data confirmed that QSc.VR4 is an effective and stable APR locus, residing on the short arm of chromosome 4H, and QSc.BR7 derived from Buloke may be an allele of reported Rrs2. High-resolution fine mapping revealed that QSc.VR4 is located in a 0.38 Mb genomic region between InDel markers 4H2282169 and 4H2665106. The gene annotation analysis and sequence comparison suggested that a gene cluster containing two adjacent multigene families encoding leucine-rich repeat receptor kinase-like proteins (LRR-RLKs) and germin-like proteins (GLPs), respectively, is likely contributing to scald resistance. Adult plant resistance (APR) governed by QSc.VR4 may confer partial levels of resistance to the fungus Rhynchosporium commune and, furthermore, be an important resource for gene pyramiding that may contribute broad-based and more durable resistance.
Functional divergence after gene duplication plays a central role in plant evolution. Among cerea... more Functional divergence after gene duplication plays a central role in plant evolution. Among cereals, only barley, wheat and rye accumulate delphinidin-derived (blue) anthocyanins in the aleurone layer of grains, but not rice, maize and sorghum. The underlying genetic basis for this natural occurence remains elusive. Here, we mapped the barley Blx1 locus involved in blue aleruone to a ~ 1.13 Mb genetic interval on chromosome 4HL, thus identifying a trigenic cluster named MbHF35 (containing HvMYB4H, HvMYC4H and HvF35H). Sequence and expression data supported the role of these genes in conferring blue-coloured (blue aleurone) grains. Synteny analyses across monocot species showed that MbHF35 has only evolved within distinct Triticeae lineages, as a result of dispersed gene duplication. Phylogeny analyses revealed a shared evolution pattern for MbHF35 in Triticeae, suggesting that these genes have co-evolved together. We also identified a Pooideae-specific flavonoid 3',5'-hydroxylase (F3'5'H) lineage, termed here Mo_F35H2 which has higher amino acid similarity with eudicot F3'5'Hs, demonstrating a scenario of convergent evolution. Indeed, selection tests identified 13 amino acid residues in Mo_F35H2 which underwent positive selection, possibly driven by protein thermostablility selection. Furthermore, by inquiring the barley germplasm there's evidence that HvMYB4H and HvMYC4H have underwent human selection. Collectively, our study favours blue aleurone as a recently evolved trait resulting from environmental adaptation. Our findings provide an evolutionary explanation for the absence of blue anthocyanins in other cereals and highlight the importance of gene functional divergence for plant diversity and environmental adaptation.
Tillering or branching is an important agronomic trait in plants, especially cereal crops. Previo... more Tillering or branching is an important agronomic trait in plants, especially cereal crops. Previously, in barley (Hordeum vulgare) 'Vlamingh', we identified the high number of tillers1 (hnt1) mutant from a gray treated segregating population. hnt1 exhibited more tillers per plant, narrower leaves, and reduced plant height compared with the wild-type parent. In this study, we show that the hnt1-increased tiller number per plant is caused by accelerated outgrowth of tiller buds and that hnt1 narrower leaves are caused by a reduction in vascular tissue and cell number. Genetic analysis revealed that a 2-bp deletion in the gene HORVU2Hr1G098820 (HvHNT1), encoding a trypsin family protein, was responsible for the hnt1 mutant phenotype. Gene function was further confirmed by transgenic complementation with HvHNT1 and RNA interference experiments. HvHNT1 was expressed in vascular tissue, leaf axils, and adventitious root primordia and shown to negatively regulate tiller development. Mutation of HvHNT1 led to the accumulation of a putative cyclophilin-type peptidyl-prolyl cis/trans-isomerase (HvPPIase), which physically interacts with the HvHNT1 protein in the nucleus of plant cells. Our data suggest that HvHNT1 controls tiller development and leaf width through HvPPIase, thus contributing to understanding of the molecular players that control tillering in barley.
Barley occupies the widest ecological area among the major cereal crops, thereby generating a hig... more Barley occupies the widest ecological area among the major cereal crops, thereby generating a high potential for adaptive genetic diversity against various environmental factors. Colored barley such as black grain barley has been suggested to result from environmental adaptation to biotic and abiotic stresses. Using one double haploid population (433 lines), plus three F5 recombinant inbred line (RIL) populations (1,009 lines), the black lemma and pericarp (Blp) gene was mapped between two Insertion/deletion (Indel) markers MC_1570156 and MC_162350 with a physical distance of 0.807 Mb, containing 21 annotated genes in the mapped interval. Whole-genome re-sequencing was performed on two Tibetan wild barley lines (X1 and W1) with black grain phenotype. The probable candidate genes for Blp were discussed based on gene functional annotation and gene sequence variation analyses. Thirteen polymorphic Indel markers covering the target genetic region were used to analyze 178 barley accessions including 49 black husk entries. Genotype-based clustering analyses showed that the black landraces of different geographical background may have evolved from a single origin. Our study represents a significant improvement on the genetic mapping of Blp and would facilitate future study on the characterization of the genetic basis underlying this interesting agronomic trait.
Leaf color is an important trait for not only controlling crop yield but also monitoring plant st... more Leaf color is an important trait for not only controlling crop yield but also monitoring plant status under temperature stress. In this study, a thermo-inducible chlorophyll-deficient mutant, named V-V-Y, was identified from a gamma-radiated population of the barley variety Vlamingh. The leaves of the mutant were green under normal growing temperature but turned yellowish under high temperature in the glasshouse experiment. The ratio of chlorophyll a and chlorophyll b in the mutant declined much faster in the first 7-9 days under heat treatment. The leaves of V-V-Y turned yellowish but took longer to senesce under heat stress in the field experiment. Genetic analysis indicated that a single nuclear gene controlled the mutant trait. The mutant gene (vvy) was mapped to the long arm of chromosome 4H between SNP markers 1_0269 and 1_1531 with a genetic distance of 2.2 cM and a physical interval of 9.85 Mb. A QTL for grain yield was mapped to the same interval and explained 10.4% of the ...
A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant... more A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H-6H each explaining 10-25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedlin...
The dwarfing gene sdw1 has been widely used throughout the world to develop commercial barley var... more The dwarfing gene sdw1 has been widely used throughout the world to develop commercial barley varieties. There are at least four different alleles at the sdw1 locus. Mutations in the gibberellin 20-oxidase gene (HvGA20ox2) resulted in multiple alleles at the sdw1 locus. The sdw1.d allele from Diamant is due to a 7-bp deletion in exon 1, while the sdw1.c allele from Abed Denso has 1-bp deletion and a 4-bp insertion in the 5' untranslated region. The sdw1.a allele from Jotun resulted from a total deletion of the HvGA20ox2 gene. The structural changes result in lower gene expression in sdw1.d and lack of expression in sdw1.a. There are three HvGA20ox genes in the barley genome. The partial or total loss of function of the HvGA20ox2 gene could be compensated by enhanced expression of its homolog HvGA20ox1and HvGA20ox3. A diagnostic molecular marker was developed to differentiate between the wild-type, sdw1.d and sdw1.a alleles and another molecular marker for differentiation of sdw1...
Barley semi-dwarf genes have been extensively explored and widely used in barley breeding program... more Barley semi-dwarf genes have been extensively explored and widely used in barley breeding programs. The semi-dwarf gene ari-e from Golden Promise is an important gene associated with some agronomic traits and salt tolerance. While ari-e has been mapped on barley chromosome 5H using traditional markers and next-generation sequencing technologies, it has not yet been finely located on this chromosome. We integrated two methods to develop molecular markers for fine-mapping the semi-dwarf gene ari-e: (1) specific-length amplified fragment sequencing (SLAF-seq) with bulked segregant analysis (BSA) to develop SNP markers, and (2) the whole-genome shotgun sequence to develop InDels. Both SNP and InDel markers were developed in the target region and used for fine-mapping the ari-e gene. Linkage analysis showed that ari-e co-segregated with marker InDel-17 and was delimited by two markers (InDel-16 and DGSNP21) spanning 6.8 cM in the doubled haploid (DH) Dash × VB9104 population. The genetic...
Rhynchosporium secalis can overcome a single resistance gene of barley in a relatively short peri... more Rhynchosporium secalis can overcome a single resistance gene of barley in a relatively short period of time. Novel genes and quantitative trait locis (QTLs) are therefore vital to control scald in barley. A population of 220 double haploid lines was developed from a cross of Vlamingh and WABAR2147, where Vlamingh showed adult plant resistance (APR) and WABAR2147 showed seedling resistance to a group of isolates. The population was tested for APR to scald under natural infection in two consecutive seasons in addition to a seedling screen with three isolates. One single gene was mapped to chromosome 6H based on the seedling test, and two QTLs (QSc.VlWa.4H and QSc.VlWa.6H) were mapped to chromosomes 4H and 6H based on APR. Epistatic interaction was observed between the two QTLs, and environment/QTL interaction was only observed for QSc.VlWa.6H which cosegregated with the seedling resistance gene and contributed to basal resistance against scald during whole growth stages. QSc.VlWa.4H explained 42.5 and 57.8 % of the phenotypic variation in the two independent trials when the effect of QSc.VlWa.6H was excluded from the analysis. We developed a high-density consensus genetic map with 7,876 molecular makers and anchored 43 QTLs and 7 genes for scald resistance from different mapping populations. No known QTLs or genes were reported in a similar position to QSc.VlWa.4H, and it was the first major QTL for APR of scald on chromosome 4HS in barley. Combination of the two QTLs achieved better and stable scald resistance across four different environments.
Over the last two GRDC supported projects - Australia China collaboration on barley germplasm, we... more Over the last two GRDC supported projects - Australia China collaboration on barley germplasm, we have accumulated a large volume of experimental data related to various aspects of waterlogging stress in barley. These included: a series physiological studies on waterlogging tolerance; genetic studies and identification of molecular markers for waterlogging tolerance; and QTL mapping for waterlogging related physiological traits. Most importantly, reliable screening facilities were developed, which enable the accurate phenotyping thus effectively breeding for the tolerance. Further research on creating germplasm with waterlogging tolerance will also be discussed.
Department of Agriculture Western Australia, 3 Baron-Hay Court, South Perth WA6151 Department of ... more Department of Agriculture Western Australia, 3 Baron-Hay Court, South Perth WA6151 Department of Primary Industries, 13 Holberton Street, Toowoomba, QLD 4350 State Agricultural Biotechnology Centre, Murdoch University, WA6150 School of Plant Biology (Plant Sciences), Faculty of Natural Agriculture, University of Western Australia, Nedlands WA6009 Department of Plant Science, University of Adelaide, Glen Osmond, SA 5064 NSW Agriculture, Wagga Wagga Agricultural Institute, PMB, Wagga Wagga NSW 2650
ABSTRACT With 2 figures and 5 tablesAbstractNet type net blotch (NTNB) is an important barley dis... more ABSTRACT With 2 figures and 5 tablesAbstractNet type net blotch (NTNB) is an important barley disease in Australia and elsewhere, with significant yield reduction. This trait is important in selection along with other traits of quality and agronomic value. Two-hundred doubled-haploid lines were generated through anther culture from a cross between ‘Pompadour’ and ‘Stirling’. Quantitative trait loci (QTL) were identified against five isolates of Pyrenophora teres f. teres, which represent virulences across Australia. QTL were mapped on chromosomes 3H and 6H using simple sequence repeat (SSR) markers. The resistance locus on 6H was detected with all isolates while the 3H locus was detected with two isolates. The 6H QTL from ‘Pompadour’ contributed resistance to isolates 97NB1, 95NB100 and NB81, whereas 6H QTL from ‘Stirling’ contributed resistance to isolates NB50 and NB52B. The 3H QTL from ‘Pompadour’ contributed resistance to NB50 and NB52B. Significant epistatic interactions were detected between QTL on 3H and 6H. These resistance QTL are a useful resource and identifying closely linked SSR markers with allelic combinations will facilitate in marker-assisted selection to develop NTNB resistant breeding lines.
ABSTRACT Acid soil/aluminium toxicity is one of the major constraints on barley production around... more ABSTRACT Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals—introduced from CYMMIT (International Maize and Wheat Improvement Center)—was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion–deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.
Photoperiod response is a key determinant for barley adaptation to diverse environments. A major ... more Photoperiod response is a key determinant for barley adaptation to diverse environments. A major quantitative trait locus (QTL) for response to long photoperiod was identified in Australia (Perth, 3156 0 S) and China (Wuhan, 3033 0 N) using 178 doubled haploid lines derived from a cross of an Australian barley, Baudin, and a Canadian barley, AC Metcalfe. The QTL was detected as a major QTL in the 18-h photope- riod glasshouse experiments and mapped to the Xp12m50B199-Xp13m47B399 interval on chromosome 4H with a LOD score of 57 in Australia and confirmed in China. The single QTL accounted for 77.48 and 37.81% of phenotypic variation for long photoperiod response in Australia and China, respectively. The same QTL also controlled heading date in Australia, under normal and extended photoperiod conditions, and in China, under extended photoperiod and late-sown conditions. The QTL advanced heading date by 27.8 days in Australia and 42.5 days in China under a 18-h photoperiod. In addition, QTL for heading date were identified on chromosomes 2H and 3H. The chromosome 3H QTL was associated with the denso gene and detected in all conditions, but the chromosome 2H QTL was only detected in Australia. The new photoperiod response QTL, Qhea.BM.4-13/Qpho.BM.4-13, on chromosome 4H and its associated markers will provide an alternative for plant breeders developing new varieties for different environments using marker-assisted selection.
The association between high malting quality and pre-harvest sprouting (PHS) susceptibility is a ... more The association between high malting quality and pre-harvest sprouting (PHS) susceptibility is a key challenge when developing new malting barley varieties. A new malting barley variety Baudin has successfully combined high malting quality and PHS tolerance. A doubled haploid population was developed for mapping PHS tolerance and seed dormancy from a cross of Baudin 9 AC Metcalfe using 233 molecular markers. Three QTLs were mapped for seed dormancy based on the standard germination test at 24, 48 and 72 h. One major QTL was mapped to the long arm of chromosome 5H controlling seed dormancy and PHS tolerance from Baudin. Two other minor QTLs were identified from Baudin on chromosomes 3 and 7H. QTL/QTL interaction was detected for seed dormancy between chromosomes 3 and 5H. The PHS tolerance allele of the 5H QTL from Baudin contributes to higher malt yield without significant impact on diastatic power, beta-glucan content and wort viscosity. QTL from Baudin provide new sources to integrate PHS tolerance and high malting quality.
This paper proposes a new method to extract pores on high resolution fingerprints. The basic idea... more This paper proposes a new method to extract pores on high resolution fingerprints. The basic idea of this method is to binarize the fingerprint images based on multi-scale morphological transformation, and then extract pores by different strategies. The closed pores are extracted by the size of connected regions, and the open pores are detected using the skeleton of valleys. The noise and false detected points are finally removed by using a comprehensive selection rule. Experimental results have shown that the proposed method can improve the accuracy of existing methods.
An effective and stable quantitative resistance locus, QSc.VR4, was fine mapped, characterized an... more An effective and stable quantitative resistance locus, QSc.VR4, was fine mapped, characterized and physically anchored to the short arm of 4H, conferring adult plant resistance to the fungus Rhynchosporium commune in barley. Scald caused by Rhynchosporium commune is one of the most destructive barley diseases worldwide. Accumulation of adult plant resistance (APR) governed by multiple resistance alleles is predicted to be effective and long-lasting against a broad spectrum of pathotypes. However, the molecular mechanisms that control APR remain poorly understood. Here, quantitative trait loci (QTL) analysis of APR and fine mapping were performed on five barley populations derived from a common parent Vlamingh, which expresses APR to scald. Two QTLs, designated QSc.VR4 and QSc.BR7, were detected from a cross between Vlamingh and Buloke. Our data confirmed that QSc.VR4 is an effective and stable APR locus, residing on the short arm of chromosome 4H, and QSc.BR7 derived from Buloke may be an allele of reported Rrs2. High-resolution fine mapping revealed that QSc.VR4 is located in a 0.38 Mb genomic region between InDel markers 4H2282169 and 4H2665106. The gene annotation analysis and sequence comparison suggested that a gene cluster containing two adjacent multigene families encoding leucine-rich repeat receptor kinase-like proteins (LRR-RLKs) and germin-like proteins (GLPs), respectively, is likely contributing to scald resistance. Adult plant resistance (APR) governed by QSc.VR4 may confer partial levels of resistance to the fungus Rhynchosporium commune and, furthermore, be an important resource for gene pyramiding that may contribute broad-based and more durable resistance.
Functional divergence after gene duplication plays a central role in plant evolution. Among cerea... more Functional divergence after gene duplication plays a central role in plant evolution. Among cereals, only barley, wheat and rye accumulate delphinidin-derived (blue) anthocyanins in the aleurone layer of grains, but not rice, maize and sorghum. The underlying genetic basis for this natural occurence remains elusive. Here, we mapped the barley Blx1 locus involved in blue aleruone to a ~ 1.13 Mb genetic interval on chromosome 4HL, thus identifying a trigenic cluster named MbHF35 (containing HvMYB4H, HvMYC4H and HvF35H). Sequence and expression data supported the role of these genes in conferring blue-coloured (blue aleurone) grains. Synteny analyses across monocot species showed that MbHF35 has only evolved within distinct Triticeae lineages, as a result of dispersed gene duplication. Phylogeny analyses revealed a shared evolution pattern for MbHF35 in Triticeae, suggesting that these genes have co-evolved together. We also identified a Pooideae-specific flavonoid 3',5'-hydroxylase (F3'5'H) lineage, termed here Mo_F35H2 which has higher amino acid similarity with eudicot F3'5'Hs, demonstrating a scenario of convergent evolution. Indeed, selection tests identified 13 amino acid residues in Mo_F35H2 which underwent positive selection, possibly driven by protein thermostablility selection. Furthermore, by inquiring the barley germplasm there's evidence that HvMYB4H and HvMYC4H have underwent human selection. Collectively, our study favours blue aleurone as a recently evolved trait resulting from environmental adaptation. Our findings provide an evolutionary explanation for the absence of blue anthocyanins in other cereals and highlight the importance of gene functional divergence for plant diversity and environmental adaptation.
Tillering or branching is an important agronomic trait in plants, especially cereal crops. Previo... more Tillering or branching is an important agronomic trait in plants, especially cereal crops. Previously, in barley (Hordeum vulgare) 'Vlamingh', we identified the high number of tillers1 (hnt1) mutant from a gray treated segregating population. hnt1 exhibited more tillers per plant, narrower leaves, and reduced plant height compared with the wild-type parent. In this study, we show that the hnt1-increased tiller number per plant is caused by accelerated outgrowth of tiller buds and that hnt1 narrower leaves are caused by a reduction in vascular tissue and cell number. Genetic analysis revealed that a 2-bp deletion in the gene HORVU2Hr1G098820 (HvHNT1), encoding a trypsin family protein, was responsible for the hnt1 mutant phenotype. Gene function was further confirmed by transgenic complementation with HvHNT1 and RNA interference experiments. HvHNT1 was expressed in vascular tissue, leaf axils, and adventitious root primordia and shown to negatively regulate tiller development. Mutation of HvHNT1 led to the accumulation of a putative cyclophilin-type peptidyl-prolyl cis/trans-isomerase (HvPPIase), which physically interacts with the HvHNT1 protein in the nucleus of plant cells. Our data suggest that HvHNT1 controls tiller development and leaf width through HvPPIase, thus contributing to understanding of the molecular players that control tillering in barley.
Barley occupies the widest ecological area among the major cereal crops, thereby generating a hig... more Barley occupies the widest ecological area among the major cereal crops, thereby generating a high potential for adaptive genetic diversity against various environmental factors. Colored barley such as black grain barley has been suggested to result from environmental adaptation to biotic and abiotic stresses. Using one double haploid population (433 lines), plus three F5 recombinant inbred line (RIL) populations (1,009 lines), the black lemma and pericarp (Blp) gene was mapped between two Insertion/deletion (Indel) markers MC_1570156 and MC_162350 with a physical distance of 0.807 Mb, containing 21 annotated genes in the mapped interval. Whole-genome re-sequencing was performed on two Tibetan wild barley lines (X1 and W1) with black grain phenotype. The probable candidate genes for Blp were discussed based on gene functional annotation and gene sequence variation analyses. Thirteen polymorphic Indel markers covering the target genetic region were used to analyze 178 barley accessions including 49 black husk entries. Genotype-based clustering analyses showed that the black landraces of different geographical background may have evolved from a single origin. Our study represents a significant improvement on the genetic mapping of Blp and would facilitate future study on the characterization of the genetic basis underlying this interesting agronomic trait.
Leaf color is an important trait for not only controlling crop yield but also monitoring plant st... more Leaf color is an important trait for not only controlling crop yield but also monitoring plant status under temperature stress. In this study, a thermo-inducible chlorophyll-deficient mutant, named V-V-Y, was identified from a gamma-radiated population of the barley variety Vlamingh. The leaves of the mutant were green under normal growing temperature but turned yellowish under high temperature in the glasshouse experiment. The ratio of chlorophyll a and chlorophyll b in the mutant declined much faster in the first 7-9 days under heat treatment. The leaves of V-V-Y turned yellowish but took longer to senesce under heat stress in the field experiment. Genetic analysis indicated that a single nuclear gene controlled the mutant trait. The mutant gene (vvy) was mapped to the long arm of chromosome 4H between SNP markers 1_0269 and 1_1531 with a genetic distance of 2.2 cM and a physical interval of 9.85 Mb. A QTL for grain yield was mapped to the same interval and explained 10.4% of the ...
A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant... more A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H-6H each explaining 10-25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedlin...
The dwarfing gene sdw1 has been widely used throughout the world to develop commercial barley var... more The dwarfing gene sdw1 has been widely used throughout the world to develop commercial barley varieties. There are at least four different alleles at the sdw1 locus. Mutations in the gibberellin 20-oxidase gene (HvGA20ox2) resulted in multiple alleles at the sdw1 locus. The sdw1.d allele from Diamant is due to a 7-bp deletion in exon 1, while the sdw1.c allele from Abed Denso has 1-bp deletion and a 4-bp insertion in the 5' untranslated region. The sdw1.a allele from Jotun resulted from a total deletion of the HvGA20ox2 gene. The structural changes result in lower gene expression in sdw1.d and lack of expression in sdw1.a. There are three HvGA20ox genes in the barley genome. The partial or total loss of function of the HvGA20ox2 gene could be compensated by enhanced expression of its homolog HvGA20ox1and HvGA20ox3. A diagnostic molecular marker was developed to differentiate between the wild-type, sdw1.d and sdw1.a alleles and another molecular marker for differentiation of sdw1...
Barley semi-dwarf genes have been extensively explored and widely used in barley breeding program... more Barley semi-dwarf genes have been extensively explored and widely used in barley breeding programs. The semi-dwarf gene ari-e from Golden Promise is an important gene associated with some agronomic traits and salt tolerance. While ari-e has been mapped on barley chromosome 5H using traditional markers and next-generation sequencing technologies, it has not yet been finely located on this chromosome. We integrated two methods to develop molecular markers for fine-mapping the semi-dwarf gene ari-e: (1) specific-length amplified fragment sequencing (SLAF-seq) with bulked segregant analysis (BSA) to develop SNP markers, and (2) the whole-genome shotgun sequence to develop InDels. Both SNP and InDel markers were developed in the target region and used for fine-mapping the ari-e gene. Linkage analysis showed that ari-e co-segregated with marker InDel-17 and was delimited by two markers (InDel-16 and DGSNP21) spanning 6.8 cM in the doubled haploid (DH) Dash × VB9104 population. The genetic...
Rhynchosporium secalis can overcome a single resistance gene of barley in a relatively short peri... more Rhynchosporium secalis can overcome a single resistance gene of barley in a relatively short period of time. Novel genes and quantitative trait locis (QTLs) are therefore vital to control scald in barley. A population of 220 double haploid lines was developed from a cross of Vlamingh and WABAR2147, where Vlamingh showed adult plant resistance (APR) and WABAR2147 showed seedling resistance to a group of isolates. The population was tested for APR to scald under natural infection in two consecutive seasons in addition to a seedling screen with three isolates. One single gene was mapped to chromosome 6H based on the seedling test, and two QTLs (QSc.VlWa.4H and QSc.VlWa.6H) were mapped to chromosomes 4H and 6H based on APR. Epistatic interaction was observed between the two QTLs, and environment/QTL interaction was only observed for QSc.VlWa.6H which cosegregated with the seedling resistance gene and contributed to basal resistance against scald during whole growth stages. QSc.VlWa.4H explained 42.5 and 57.8 % of the phenotypic variation in the two independent trials when the effect of QSc.VlWa.6H was excluded from the analysis. We developed a high-density consensus genetic map with 7,876 molecular makers and anchored 43 QTLs and 7 genes for scald resistance from different mapping populations. No known QTLs or genes were reported in a similar position to QSc.VlWa.4H, and it was the first major QTL for APR of scald on chromosome 4HS in barley. Combination of the two QTLs achieved better and stable scald resistance across four different environments.
Over the last two GRDC supported projects - Australia China collaboration on barley germplasm, we... more Over the last two GRDC supported projects - Australia China collaboration on barley germplasm, we have accumulated a large volume of experimental data related to various aspects of waterlogging stress in barley. These included: a series physiological studies on waterlogging tolerance; genetic studies and identification of molecular markers for waterlogging tolerance; and QTL mapping for waterlogging related physiological traits. Most importantly, reliable screening facilities were developed, which enable the accurate phenotyping thus effectively breeding for the tolerance. Further research on creating germplasm with waterlogging tolerance will also be discussed.
Department of Agriculture Western Australia, 3 Baron-Hay Court, South Perth WA6151 Department of ... more Department of Agriculture Western Australia, 3 Baron-Hay Court, South Perth WA6151 Department of Primary Industries, 13 Holberton Street, Toowoomba, QLD 4350 State Agricultural Biotechnology Centre, Murdoch University, WA6150 School of Plant Biology (Plant Sciences), Faculty of Natural Agriculture, University of Western Australia, Nedlands WA6009 Department of Plant Science, University of Adelaide, Glen Osmond, SA 5064 NSW Agriculture, Wagga Wagga Agricultural Institute, PMB, Wagga Wagga NSW 2650
ABSTRACT With 2 figures and 5 tablesAbstractNet type net blotch (NTNB) is an important barley dis... more ABSTRACT With 2 figures and 5 tablesAbstractNet type net blotch (NTNB) is an important barley disease in Australia and elsewhere, with significant yield reduction. This trait is important in selection along with other traits of quality and agronomic value. Two-hundred doubled-haploid lines were generated through anther culture from a cross between ‘Pompadour’ and ‘Stirling’. Quantitative trait loci (QTL) were identified against five isolates of Pyrenophora teres f. teres, which represent virulences across Australia. QTL were mapped on chromosomes 3H and 6H using simple sequence repeat (SSR) markers. The resistance locus on 6H was detected with all isolates while the 3H locus was detected with two isolates. The 6H QTL from ‘Pompadour’ contributed resistance to isolates 97NB1, 95NB100 and NB81, whereas 6H QTL from ‘Stirling’ contributed resistance to isolates NB50 and NB52B. The 3H QTL from ‘Pompadour’ contributed resistance to NB50 and NB52B. Significant epistatic interactions were detected between QTL on 3H and 6H. These resistance QTL are a useful resource and identifying closely linked SSR markers with allelic combinations will facilitate in marker-assisted selection to develop NTNB resistant breeding lines.
ABSTRACT Acid soil/aluminium toxicity is one of the major constraints on barley production around... more ABSTRACT Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals—introduced from CYMMIT (International Maize and Wheat Improvement Center)—was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion–deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.
Photoperiod response is a key determinant for barley adaptation to diverse environments. A major ... more Photoperiod response is a key determinant for barley adaptation to diverse environments. A major quantitative trait locus (QTL) for response to long photoperiod was identified in Australia (Perth, 3156 0 S) and China (Wuhan, 3033 0 N) using 178 doubled haploid lines derived from a cross of an Australian barley, Baudin, and a Canadian barley, AC Metcalfe. The QTL was detected as a major QTL in the 18-h photope- riod glasshouse experiments and mapped to the Xp12m50B199-Xp13m47B399 interval on chromosome 4H with a LOD score of 57 in Australia and confirmed in China. The single QTL accounted for 77.48 and 37.81% of phenotypic variation for long photoperiod response in Australia and China, respectively. The same QTL also controlled heading date in Australia, under normal and extended photoperiod conditions, and in China, under extended photoperiod and late-sown conditions. The QTL advanced heading date by 27.8 days in Australia and 42.5 days in China under a 18-h photoperiod. In addition, QTL for heading date were identified on chromosomes 2H and 3H. The chromosome 3H QTL was associated with the denso gene and detected in all conditions, but the chromosome 2H QTL was only detected in Australia. The new photoperiod response QTL, Qhea.BM.4-13/Qpho.BM.4-13, on chromosome 4H and its associated markers will provide an alternative for plant breeders developing new varieties for different environments using marker-assisted selection.
The association between high malting quality and pre-harvest sprouting (PHS) susceptibility is a ... more The association between high malting quality and pre-harvest sprouting (PHS) susceptibility is a key challenge when developing new malting barley varieties. A new malting barley variety Baudin has successfully combined high malting quality and PHS tolerance. A doubled haploid population was developed for mapping PHS tolerance and seed dormancy from a cross of Baudin 9 AC Metcalfe using 233 molecular markers. Three QTLs were mapped for seed dormancy based on the standard germination test at 24, 48 and 72 h. One major QTL was mapped to the long arm of chromosome 5H controlling seed dormancy and PHS tolerance from Baudin. Two other minor QTLs were identified from Baudin on chromosomes 3 and 7H. QTL/QTL interaction was detected for seed dormancy between chromosomes 3 and 5H. The PHS tolerance allele of the 5H QTL from Baudin contributes to higher malt yield without significant impact on diastatic power, beta-glucan content and wort viscosity. QTL from Baudin provide new sources to integrate PHS tolerance and high malting quality.
Ebook ini adalah buku penulis yang secara khusus bicarakan tentang Manajemen yang diperkayakan d... more Ebook ini adalah buku penulis yang secara khusus bicarakan tentang Manajemen yang diperkayakan dengan tulisan lain dalam bidang pendidikan. melihat isi buku, penulis memberikan nama karya dengan judul “Pengantar Manajemen”. Ebook ini berisi tentang bahan ajar yang akan diajarkan diperguruan tinggi dengan kajian-kajian sebagai berikut.
Memberikan Pemahaman berbagai usaha mencapai suatu tujuan tertentu melalui kegiatanorang lain, juga dapat mengetahui pentingnya manajemen bagi pengelolaan organisasi, aspek-aspek yang dilakukan manajemen serta fungsi-fungsi manajemen yang meliputi perencanaan, pengorganisasian, koordinasi, motivasi karyawan, pengawasan termasuk juga pengambilan keputusan.
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Ebook ini berisi tentang bahan ajar yang akan diajarkan diperguruan tinggi dengan kajian-kajian sebagai berikut.
Memberikan Pemahaman berbagai usaha mencapai suatu tujuan tertentu melalui kegiatanorang lain, juga dapat mengetahui pentingnya manajemen bagi pengelolaan organisasi, aspek-aspek yang dilakukan manajemen serta fungsi-fungsi manajemen yang meliputi perencanaan, pengorganisasian, koordinasi, motivasi karyawan, pengawasan termasuk juga pengambilan keputusan.