Papers by Danna Rodriguez
Journal of Microbiology, Immunology and Infection, 2015
Purpose: Escherichia coli sero-group O25b-sequence type 131 (O25b-ST131), a multi-drug-resistant ... more Purpose: Escherichia coli sero-group O25b-sequence type 131 (O25b-ST131), a multi-drug-resistant clonal group, is a significant pathogen in adults and children. This study investigated the genotyping and role of extended-spectrum b-lactamase (ESBL)-producing E. coli O25b-ST131 and non-O25b-ST131 in urinary tract infections (UTI) in children. Methods: We randomly enrolled one third of all the children (n Z 363) under 18 years of age who were hospitalized for UTI caused by ESBL-producing E. coli between 2009 and 2012. Clinical and laboratory data from the enrolled 121 children were collected. Polymerase chain reactions and multi-locus sequence typing were used to identify E. coli O25-ST131 clones. The gene blaCTX-M groups 1, 2, and 9, a specific PCR reaction of CTX-M 14 and 15, were also determined in ESBL-producing E. coli isolates. Results: O25b-ST131 accounted for 57 (47%) of the 121 isolates, while 54 isolates belonged to the blaCTX-M group 9, of which most were CTX-M-14. Among the 64 non-O25b-ST131 isolates, 10 isolates belonged to the blaCTX-M group 1, of which seven were CTX-M-14; three isolates belonged to the blaCTX-M group 2 and fourteen isolates belonged to the blaCTX-M group 9, of which ten were CTX-M-14. Those with O25b-ST131 clones had similar risk factors, clinical features, and outcomes as those with non-O25b-ST131. The O25b-ST131 isolates were more resistant to ciprofloxacin
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Papers by Danna Rodriguez