The professionalism of the Nuremberg interpreters highlights shortcomings in current UK interpret... more The professionalism of the Nuremberg interpreters highlights shortcomings in current UK interpreting standards.
We read with interest the article by Reyes et al (1999) describing a transient suppression of lym... more We read with interest the article by Reyes et al (1999) describing a transient suppression of lymphocyte proliferative response to polyclonal mitogens in patients affected by breast cancer and receiving rHuG-CSF to mobilize peripheral blood progenitor cells (PBPC); these variations occurred irrespective of changes in both T-cell subset distribution and lymphocyte ex vivo 'activation status', as measured by expression of HLA-DR and CD25 activation-related antigens. Data reported by Reyes et al confirm that G-CSF treatment modulates the proliferative response of peripheral blood mononuclear cells to T-cell mitogens in humans. Investigations performed by our group in healthy subjects treated with rHuG-CSF to mobilize PBPC have already demonstrated a humoralmediated rHuG-CSF-induced suppression of lymphocyte cycling after mitogenic challenge (Rutella et al, 1997; 1997); interestingly, lymphocyte proliferation inversely correlated with increased serum levels of immunoregulatory cytokines, i.e. lactoferrin and interleukin-1 receptor antagonist (IL-1ra), strongly suggesting indirect actions mediated by soluble factors; this hypothesis is further supported by the observation that lymphocytes apparently lack G-CSF receptors (Mellstedt et al, 1999; Hartung, 1999). We next evaluated the effects of serum collected after rHuG-CSF administration (G-serum) on the proliferation of normal allogeneic lymphocytes (Rutella et al, 1998); when PHAchallenge was performed in the presence of G-serum, lymphocyte cycling was strongly inhibited in a concentration-dependent manner and lymphocytes were arrested in a G0-like phase of the cell cycle, although they up-regulated the expression of early (CD69) and late activation related antigens (CD25, CD71 and HLA-DR), enlarged into blasts and secreted normal amounts of IL-2. Taken together, these features recapitulate the phenotype known as lymphocyte partial activation, which consists of: (1) preserved blast transformation, (2) preserved up-regulation of activation-related antigens, i.e. CD69, CD25, CD71 and HLA-DR, (3) preserved secretion of IL-2 and (4) inability to progress through the cell cycle upon mitogenic stimulation. Lymphocyte partial activation has been regarded as a fundamental mechanism for tolerance induction in T-cell clones and might control the amplification of the immune response (Sloan-Lancaster et al, 1994). From our data (Rutella et al, 1999) and those by other groups (Hartung, 1999), a new intriguing role of rHuG-CSF as an immune suppressive agent has emerged. The modulation of immune responses might be beneficial after the infusion of rHuG-CSF-mobilized allogeneic PBPC; conceivably, similar incidence and severity of acute graft versus host disease (GVHD), which has been recently reported after allogeneic PBPC compared to bone marrow transplantation, might be explained by a functional alteration of lymphocytes exposed in vivo to rHuG-CSF.
Objectives 1) To assess the different clinical varieties and the evolution of the patients. 2) To... more Objectives 1) To assess the different clinical varieties and the evolution of the patients. 2) To assess the clinical impact of the use of high dose corticotherapy. 3) To analyze the phenotype of the lymphocyte populations in peripheral blood. 4) To study the proliferative answer of mononuclear cells in peripheral blood under polyclonal mitogen stimulus. Methods A prospective study in patients with immunomediated inner ear disease was carried out, dividing them in 4 different clinical presentations: Sudden deafness, fluctuant hearing loss, Meniere's disease, and rapidly progressive hearing loss. We analysed 22 patients, controlling their clinical evolution and response to corticotherapy. We also made an immunophenotypical and functional characterization of the T lymphocytes in patients before and after treatment and comparing them with healthy controls. Mann-Whitney U and Wilcoxon range tests were used as statistical resources. Results 59.09% of the patients treated with cortico...
Se analizó el patrón de segmentación embrionaria en el hámster sirio dorado (Mesocricetus auratus... more Se analizó el patrón de segmentación embrionaria en el hámster sirio dorado (Mesocricetus auratus) utilizando dos grupos de hembras: 47 hembras nulíparas de 3-4 meses de edad y 39 multíparas (3 partos en promedio) de 7-8 meses de edad. El tiempo de apareamiento se restringió a 15 minutos, y el tiempo de la ovulación se determinó con base en estudios secuenciales de citología vaginal exfoliativa. El mejor momento para obtener embriones de 8 blastómeros se presentó entre las 62 y las 63 horas postcoito en las hembras nulíparas. En las hembras multíparas el tiempo de segmentación fue 2 horas mayor que en las nulíparas. Estos resultados nos permiten proponer que el aumento en la duración de la gestación, que se ha correlacionado con la edad de las hembras, ocurre ya desde las primeras etapas del desarrollo del embrión. Para precisar con exactitud el tiempo de segmentación temprana en el hámster sirio dorado, es indispensable restringir el tiempo de apareamiento y relacionarlo de una man...
Mast cells may be recruited by tumor-derived chemoattractants and selectively secrete molecules s... more Mast cells may be recruited by tumor-derived chemoattractants and selectively secrete molecules such as VEGF that permit the formation of new blood vessels and metastases. We analyze the VEGF-A and...
Footbridge 2017 Berlin - Tell A Story: Conference Proceedings 6-8.9.2017 TU-Berlin, 2017
The Cuernavaca city is located 80 KM, south of Mexico City. The highway Mexico-Acapulco pass thro... more The Cuernavaca city is located 80 KM, south of Mexico City. The highway Mexico-Acapulco pass through the Cuernavaca city, and traffic jams are very important, particularly during weekends and holydays. For that reason the Ministry of Transports of Mexico started a program to expand the highway from 4 to 10 circulation lanes in the 14.5 KM of the highway zone that crosses the city of Cuernavaca. In that context 10 footbridges, placed over the highway were demolished and replaced with new ones with spans from 37 to 47 m. The bridges are arch type in steel. This type of structure was selected for aesthetic and structural reasons. Due to the high flexibility of the bridges, the dynamic behavior under pedestrian loading was studied and modifications to the original design were made in order to avoid unacceptable vibrations. The bridges are also placed in a high seismic region, and time history calculations considering non-linear behavior of the concrete piers were made in order to evalua...
e21593 Background: Non-Small cell lung cancer (NSCLC) is the most common type of lung cancer and ... more e21593 Background: Non-Small cell lung cancer (NSCLC) is the most common type of lung cancer and accounts for most of all cancer-related morbidity and deaths in the World. Recent Evidence shows that inflammatory response is associated with a poor prognostic in several cancers. Evaluating these markers is of great importance to classify patients of solid tumors including NSCLC. Inflammatory markers like, Glasgow Prognostic Score (GPS), Lung Immune prognostic index (LIPI) and C-Reactive Protein (CRP) have been associated with poor prognosis in patients treated with immune checkpoint inhibitors. Neutrophil to Lymphocyte Ratio (NLR) is a biomarker for the general immune response to various stress stimuli in peripheral blood. It can be easily determined, inexpensive and can correlate with poor outcomes. Methods: A review of medical records was performed including patients from January 2013 to December 2018. The clinical characteristics were described, analyzed and the NLR and the LIPI we...
Recent studies have indicated that autoimmune thrombocytopenic purpura (ATP) patients show immune... more Recent studies have indicated that autoimmune thrombocytopenic purpura (ATP) patients show immune system alterations that are not restricted to the B-cell compartment, but that also affect T lymphocytes. This report studies the phenotypic characteristics of natural killer (NK) cells in the peripheral blood of ATP patients, as well as their clinical significance in 33 ATP patients with active disease. Ten patients had stable disease (sustained platelet counts > 50,000/microL without the need for treatment), whereas 23 patients had therapy- dependent disease (platelet counts < 50,000/microL). A significant increase in both CD56+ CD3- NK cells and CD56+ CD3+ cytotoxic T lymphocytes was observed in peripheral blood mononuclear cells and in purified CD2+ cells from therapy-dependent ATP patients as compared with ATP patients with stable disease and healthy controls. Moreover, there were more major histocompatibility complex (MHC) class II molecules in the CD56+ CD3- cells from the ...
This paper studies the phenotypical characteristics of the lymphocytes present in mononuclear cel... more This paper studies the phenotypical characteristics of the lymphocytes present in mononuclear cell (MNC) preparations from peripheral blood (PBMNC), lymph nodes (LNMNC), tumor bladder (TBMNC), and tumor-free bladder (TFBMNC) from patients with infiltrated transitional cell carcinoma (TCC) of the bladder and PBMNC of healthy controls. Eight patients diagnosed with TCC of the bladder, according to UICC criteria, and 10 healthy controls were studied. For immunofluorescence staining, T lymphocytes were incubated with combinations of fluorescein (FITC, green)- and phycoerytrin (PE, red)-labelled monoclonal antibodies. The percentage of NK cells in the LNMNC and TFBMNC was significantly decreased in comparison to that found in the PBMNC from these patients (p &amp;amp;lt; 0.05). A significant enhancement of the expression of class II molecules of the major histocompatibility complex (MHC) by CD3+ T lymphocytes from TBMNC and TFBMNC specimens from bladder walls was found with respect to those quantified in CD3+ T lymphocytes from PBMNC (p &amp;amp;lt; 0.05). In addition, the percentage of CD3+CD25+ T lymphocytes was significantly higher in PBMNC in TCC patients than in healthy controls (p &amp;amp;lt; 0.05). Our data clearly demonstrate that the presence of infiltrative TCC of the bladder is associated to an infiltration of in vivo activated T lymphocytes of the urinary bladder wall. This in vivo T lymphocyte activation has been considered an expression of the immune response against the tumor cells.
CFT1R, the protein defective in cystic fibrosis is regulated during differentiation of intestinal... more CFT1R, the protein defective in cystic fibrosis is regulated during differentiation of intestinal epithelial cells. The undifferentiated cells (Caco-2 and HT-29) show a lower level of CFTR mRNA, while a 10-fold increase is seen in differentiated cells. These differences correlate well with those of other intestinal-specific genes, including sucrase-isomaltase, villin and a 1-antitrypsin, indicating that the regulation is cell specific. In Caco-2 cells the increase in CFTrR mRNA cannot be accounted for by increased transcription of the gene. These data indicate that CFVJR mRNA stabilizing factor(s) might be present in differentiated cells. The higher levels of CFTR mRNA in differentiated cells are accompanied by decreased protein levels, indicating, as well, involvement of translational control in the regulation of CFTR in these cells. Finally, fully differentiated cells show lowered levels of cyclic AMP-activated Cltransport, the characteristic function of CFIR. Thus, CFTrR function in differentiated cells is modulated by a complex interaction of regulatory elements. Caco-2 and HT-29 cells provide a suitable in vitro system in which to study the mechanism of regulation of CFIR.
American Journal of Physiology-Cell Physiology, 1992
In this study we investigated the properties of adenosine 3',5'-cyclic monophosphate (cAM... more In this study we investigated the properties of adenosine 3',5'-cyclic monophosphate (cAMP)-stimulated Cl- efflux in Caco-2 monolayers by measuring 125I efflux rates from preloaded cells and using patch-clamp electrophysiology. The addition of a cocktail containing 100 microM dibutyryl cAMP (DBcAMP), 10 microM forskolin, and 1 mM 3-isobutyl-1-methylxanthine caused a significant (P less than 0.05) increase in the rate of 125I efflux. Dissipation of cell potential by adding valinomycin (4.5 microM) with 135 mM extracellular KCl reduced the cAMP-evoked 125I efflux. These results suggest that cAMP-stimulated anion efflux occurs through a conductive pore or channel. Whole cell currents evoked with DBcAMP or forskolin were anion selective, PCl greater than PI greater than Pgluconate, and exhibited a linear current-voltage (I-V) relationship. Currents evoked with depolarizing or hyperpolarizing voltage steps showed no evidence of time-dependent activation or inactivation. Single Cl...
Developmental and comparative immunology, Nov 20, 2018
Immunoglobulin molecules play an important role in the immune defense system in all jawed vertebr... more Immunoglobulin molecules play an important role in the immune defense system in all jawed vertebrates, by protecting the organism from a wide variety of pathogens. Nile tilapia (Oreochromis niloticus) is extensively cultivated worldwide, with a strong established market demand. It constitutes one of the model species for the study of fish immunology and its genome is currently fully sequenced. The presence of the immunoglobulin M gene in this species is well documented, as well as its major role in systemic immunity. To date, the IgT gene from O. niloticus has not been identified and, therefore, no information is available on the role of this immunoglobulin isotype in the immune response in tilapia. In the present work, novel secreted and membrane immunoglobulin T isotypes and a fragment of IgM were isolated from tilapia head kidney lymphocytes. Their transcriptional profiles were analyzed by quantitative PCR in larval development and in different tissues of healthy or lipopolysacch...
Screening for prostate cancer remains controversial; the use of circulating prostate cells could ... more Screening for prostate cancer remains controversial; the use of circulating prostate cells could be an ideal biomarker to detect prostate cancer. However, different methods have produced differing results. We review these methods and the published results, comparing with the use of standard immunocytochemistry and double immunomarcation with anti-PSA and anti-P504S to define a primary malignant circulating prostate cell. It´s use as a sequential test in men with an elevated total serum PSA and before a prostate biopsy. We review the results comparing with total serum PSA, PSA based parameters and nomograms. To cite this article: Nigel P. Murray, et al. Sequential use of primary malignant circulating prostate cells to detect prostate cancer. Can Cell Microenviron 2015; 2: e714.
This article provides an overview of four radiological accidents in Latin America, and includes a... more This article provides an overview of four radiological accidents in Latin America, and includes a history of the events, the clinical manifestations and health consequences for the exposed individuals, the medical response based on preclinical studies and the role of the International Atomic Energy Agency (IAEA) in coordinating medical response assistance.
We investigated the potential existence of differences in the distribution of T-lymphocyte subset... more We investigated the potential existence of differences in the distribution of T-lymphocyte subsets and in the proliferative response of these CD2+ cells to polyclonal mitogens in patients with transitional cell bladder carcinoma (SBTCC) treated with prophylactic intracavitary instillations of bacillus Calmette-Guérin (BCG) according to their clinical response to this treatment. Before BCG treatment, different subset distribution (CD8+ and CD3+ CD56+), activation antigen expression (CD3+ HLA-DR+) and proliferative response to mitogenic signals were found in CD2+ cells from SBTCC patients prophylactically treated with BCG who remained free of disease or those who had recurrence of tumour. Otherwise, the prophylactic intracavitary BCG instillations in SBTCC patients are associated with a transitory variation of T-lymphocyte subset distribution (CD4 and CD8) and activation antigens expression (CD25).
Corrigendum to "Increased expression of cytokines, soluble cytokine receptors, soluble apoptosis ... more Corrigendum to "Increased expression of cytokines, soluble cytokine receptors, soluble apoptosis ligand and apoptosis in dengue" [Virology 452-453 (2014) 42-51]
The professionalism of the Nuremberg interpreters highlights shortcomings in current UK interpret... more The professionalism of the Nuremberg interpreters highlights shortcomings in current UK interpreting standards.
We read with interest the article by Reyes et al (1999) describing a transient suppression of lym... more We read with interest the article by Reyes et al (1999) describing a transient suppression of lymphocyte proliferative response to polyclonal mitogens in patients affected by breast cancer and receiving rHuG-CSF to mobilize peripheral blood progenitor cells (PBPC); these variations occurred irrespective of changes in both T-cell subset distribution and lymphocyte ex vivo 'activation status', as measured by expression of HLA-DR and CD25 activation-related antigens. Data reported by Reyes et al confirm that G-CSF treatment modulates the proliferative response of peripheral blood mononuclear cells to T-cell mitogens in humans. Investigations performed by our group in healthy subjects treated with rHuG-CSF to mobilize PBPC have already demonstrated a humoralmediated rHuG-CSF-induced suppression of lymphocyte cycling after mitogenic challenge (Rutella et al, 1997; 1997); interestingly, lymphocyte proliferation inversely correlated with increased serum levels of immunoregulatory cytokines, i.e. lactoferrin and interleukin-1 receptor antagonist (IL-1ra), strongly suggesting indirect actions mediated by soluble factors; this hypothesis is further supported by the observation that lymphocytes apparently lack G-CSF receptors (Mellstedt et al, 1999; Hartung, 1999). We next evaluated the effects of serum collected after rHuG-CSF administration (G-serum) on the proliferation of normal allogeneic lymphocytes (Rutella et al, 1998); when PHAchallenge was performed in the presence of G-serum, lymphocyte cycling was strongly inhibited in a concentration-dependent manner and lymphocytes were arrested in a G0-like phase of the cell cycle, although they up-regulated the expression of early (CD69) and late activation related antigens (CD25, CD71 and HLA-DR), enlarged into blasts and secreted normal amounts of IL-2. Taken together, these features recapitulate the phenotype known as lymphocyte partial activation, which consists of: (1) preserved blast transformation, (2) preserved up-regulation of activation-related antigens, i.e. CD69, CD25, CD71 and HLA-DR, (3) preserved secretion of IL-2 and (4) inability to progress through the cell cycle upon mitogenic stimulation. Lymphocyte partial activation has been regarded as a fundamental mechanism for tolerance induction in T-cell clones and might control the amplification of the immune response (Sloan-Lancaster et al, 1994). From our data (Rutella et al, 1999) and those by other groups (Hartung, 1999), a new intriguing role of rHuG-CSF as an immune suppressive agent has emerged. The modulation of immune responses might be beneficial after the infusion of rHuG-CSF-mobilized allogeneic PBPC; conceivably, similar incidence and severity of acute graft versus host disease (GVHD), which has been recently reported after allogeneic PBPC compared to bone marrow transplantation, might be explained by a functional alteration of lymphocytes exposed in vivo to rHuG-CSF.
Objectives 1) To assess the different clinical varieties and the evolution of the patients. 2) To... more Objectives 1) To assess the different clinical varieties and the evolution of the patients. 2) To assess the clinical impact of the use of high dose corticotherapy. 3) To analyze the phenotype of the lymphocyte populations in peripheral blood. 4) To study the proliferative answer of mononuclear cells in peripheral blood under polyclonal mitogen stimulus. Methods A prospective study in patients with immunomediated inner ear disease was carried out, dividing them in 4 different clinical presentations: Sudden deafness, fluctuant hearing loss, Meniere's disease, and rapidly progressive hearing loss. We analysed 22 patients, controlling their clinical evolution and response to corticotherapy. We also made an immunophenotypical and functional characterization of the T lymphocytes in patients before and after treatment and comparing them with healthy controls. Mann-Whitney U and Wilcoxon range tests were used as statistical resources. Results 59.09% of the patients treated with cortico...
Se analizó el patrón de segmentación embrionaria en el hámster sirio dorado (Mesocricetus auratus... more Se analizó el patrón de segmentación embrionaria en el hámster sirio dorado (Mesocricetus auratus) utilizando dos grupos de hembras: 47 hembras nulíparas de 3-4 meses de edad y 39 multíparas (3 partos en promedio) de 7-8 meses de edad. El tiempo de apareamiento se restringió a 15 minutos, y el tiempo de la ovulación se determinó con base en estudios secuenciales de citología vaginal exfoliativa. El mejor momento para obtener embriones de 8 blastómeros se presentó entre las 62 y las 63 horas postcoito en las hembras nulíparas. En las hembras multíparas el tiempo de segmentación fue 2 horas mayor que en las nulíparas. Estos resultados nos permiten proponer que el aumento en la duración de la gestación, que se ha correlacionado con la edad de las hembras, ocurre ya desde las primeras etapas del desarrollo del embrión. Para precisar con exactitud el tiempo de segmentación temprana en el hámster sirio dorado, es indispensable restringir el tiempo de apareamiento y relacionarlo de una man...
Mast cells may be recruited by tumor-derived chemoattractants and selectively secrete molecules s... more Mast cells may be recruited by tumor-derived chemoattractants and selectively secrete molecules such as VEGF that permit the formation of new blood vessels and metastases. We analyze the VEGF-A and...
Footbridge 2017 Berlin - Tell A Story: Conference Proceedings 6-8.9.2017 TU-Berlin, 2017
The Cuernavaca city is located 80 KM, south of Mexico City. The highway Mexico-Acapulco pass thro... more The Cuernavaca city is located 80 KM, south of Mexico City. The highway Mexico-Acapulco pass through the Cuernavaca city, and traffic jams are very important, particularly during weekends and holydays. For that reason the Ministry of Transports of Mexico started a program to expand the highway from 4 to 10 circulation lanes in the 14.5 KM of the highway zone that crosses the city of Cuernavaca. In that context 10 footbridges, placed over the highway were demolished and replaced with new ones with spans from 37 to 47 m. The bridges are arch type in steel. This type of structure was selected for aesthetic and structural reasons. Due to the high flexibility of the bridges, the dynamic behavior under pedestrian loading was studied and modifications to the original design were made in order to avoid unacceptable vibrations. The bridges are also placed in a high seismic region, and time history calculations considering non-linear behavior of the concrete piers were made in order to evalua...
e21593 Background: Non-Small cell lung cancer (NSCLC) is the most common type of lung cancer and ... more e21593 Background: Non-Small cell lung cancer (NSCLC) is the most common type of lung cancer and accounts for most of all cancer-related morbidity and deaths in the World. Recent Evidence shows that inflammatory response is associated with a poor prognostic in several cancers. Evaluating these markers is of great importance to classify patients of solid tumors including NSCLC. Inflammatory markers like, Glasgow Prognostic Score (GPS), Lung Immune prognostic index (LIPI) and C-Reactive Protein (CRP) have been associated with poor prognosis in patients treated with immune checkpoint inhibitors. Neutrophil to Lymphocyte Ratio (NLR) is a biomarker for the general immune response to various stress stimuli in peripheral blood. It can be easily determined, inexpensive and can correlate with poor outcomes. Methods: A review of medical records was performed including patients from January 2013 to December 2018. The clinical characteristics were described, analyzed and the NLR and the LIPI we...
Recent studies have indicated that autoimmune thrombocytopenic purpura (ATP) patients show immune... more Recent studies have indicated that autoimmune thrombocytopenic purpura (ATP) patients show immune system alterations that are not restricted to the B-cell compartment, but that also affect T lymphocytes. This report studies the phenotypic characteristics of natural killer (NK) cells in the peripheral blood of ATP patients, as well as their clinical significance in 33 ATP patients with active disease. Ten patients had stable disease (sustained platelet counts > 50,000/microL without the need for treatment), whereas 23 patients had therapy- dependent disease (platelet counts < 50,000/microL). A significant increase in both CD56+ CD3- NK cells and CD56+ CD3+ cytotoxic T lymphocytes was observed in peripheral blood mononuclear cells and in purified CD2+ cells from therapy-dependent ATP patients as compared with ATP patients with stable disease and healthy controls. Moreover, there were more major histocompatibility complex (MHC) class II molecules in the CD56+ CD3- cells from the ...
This paper studies the phenotypical characteristics of the lymphocytes present in mononuclear cel... more This paper studies the phenotypical characteristics of the lymphocytes present in mononuclear cell (MNC) preparations from peripheral blood (PBMNC), lymph nodes (LNMNC), tumor bladder (TBMNC), and tumor-free bladder (TFBMNC) from patients with infiltrated transitional cell carcinoma (TCC) of the bladder and PBMNC of healthy controls. Eight patients diagnosed with TCC of the bladder, according to UICC criteria, and 10 healthy controls were studied. For immunofluorescence staining, T lymphocytes were incubated with combinations of fluorescein (FITC, green)- and phycoerytrin (PE, red)-labelled monoclonal antibodies. The percentage of NK cells in the LNMNC and TFBMNC was significantly decreased in comparison to that found in the PBMNC from these patients (p &amp;amp;lt; 0.05). A significant enhancement of the expression of class II molecules of the major histocompatibility complex (MHC) by CD3+ T lymphocytes from TBMNC and TFBMNC specimens from bladder walls was found with respect to those quantified in CD3+ T lymphocytes from PBMNC (p &amp;amp;lt; 0.05). In addition, the percentage of CD3+CD25+ T lymphocytes was significantly higher in PBMNC in TCC patients than in healthy controls (p &amp;amp;lt; 0.05). Our data clearly demonstrate that the presence of infiltrative TCC of the bladder is associated to an infiltration of in vivo activated T lymphocytes of the urinary bladder wall. This in vivo T lymphocyte activation has been considered an expression of the immune response against the tumor cells.
CFT1R, the protein defective in cystic fibrosis is regulated during differentiation of intestinal... more CFT1R, the protein defective in cystic fibrosis is regulated during differentiation of intestinal epithelial cells. The undifferentiated cells (Caco-2 and HT-29) show a lower level of CFTR mRNA, while a 10-fold increase is seen in differentiated cells. These differences correlate well with those of other intestinal-specific genes, including sucrase-isomaltase, villin and a 1-antitrypsin, indicating that the regulation is cell specific. In Caco-2 cells the increase in CFTrR mRNA cannot be accounted for by increased transcription of the gene. These data indicate that CFVJR mRNA stabilizing factor(s) might be present in differentiated cells. The higher levels of CFTR mRNA in differentiated cells are accompanied by decreased protein levels, indicating, as well, involvement of translational control in the regulation of CFTR in these cells. Finally, fully differentiated cells show lowered levels of cyclic AMP-activated Cltransport, the characteristic function of CFIR. Thus, CFTrR function in differentiated cells is modulated by a complex interaction of regulatory elements. Caco-2 and HT-29 cells provide a suitable in vitro system in which to study the mechanism of regulation of CFIR.
American Journal of Physiology-Cell Physiology, 1992
In this study we investigated the properties of adenosine 3',5'-cyclic monophosphate (cAM... more In this study we investigated the properties of adenosine 3',5'-cyclic monophosphate (cAMP)-stimulated Cl- efflux in Caco-2 monolayers by measuring 125I efflux rates from preloaded cells and using patch-clamp electrophysiology. The addition of a cocktail containing 100 microM dibutyryl cAMP (DBcAMP), 10 microM forskolin, and 1 mM 3-isobutyl-1-methylxanthine caused a significant (P less than 0.05) increase in the rate of 125I efflux. Dissipation of cell potential by adding valinomycin (4.5 microM) with 135 mM extracellular KCl reduced the cAMP-evoked 125I efflux. These results suggest that cAMP-stimulated anion efflux occurs through a conductive pore or channel. Whole cell currents evoked with DBcAMP or forskolin were anion selective, PCl greater than PI greater than Pgluconate, and exhibited a linear current-voltage (I-V) relationship. Currents evoked with depolarizing or hyperpolarizing voltage steps showed no evidence of time-dependent activation or inactivation. Single Cl...
Developmental and comparative immunology, Nov 20, 2018
Immunoglobulin molecules play an important role in the immune defense system in all jawed vertebr... more Immunoglobulin molecules play an important role in the immune defense system in all jawed vertebrates, by protecting the organism from a wide variety of pathogens. Nile tilapia (Oreochromis niloticus) is extensively cultivated worldwide, with a strong established market demand. It constitutes one of the model species for the study of fish immunology and its genome is currently fully sequenced. The presence of the immunoglobulin M gene in this species is well documented, as well as its major role in systemic immunity. To date, the IgT gene from O. niloticus has not been identified and, therefore, no information is available on the role of this immunoglobulin isotype in the immune response in tilapia. In the present work, novel secreted and membrane immunoglobulin T isotypes and a fragment of IgM were isolated from tilapia head kidney lymphocytes. Their transcriptional profiles were analyzed by quantitative PCR in larval development and in different tissues of healthy or lipopolysacch...
Screening for prostate cancer remains controversial; the use of circulating prostate cells could ... more Screening for prostate cancer remains controversial; the use of circulating prostate cells could be an ideal biomarker to detect prostate cancer. However, different methods have produced differing results. We review these methods and the published results, comparing with the use of standard immunocytochemistry and double immunomarcation with anti-PSA and anti-P504S to define a primary malignant circulating prostate cell. It´s use as a sequential test in men with an elevated total serum PSA and before a prostate biopsy. We review the results comparing with total serum PSA, PSA based parameters and nomograms. To cite this article: Nigel P. Murray, et al. Sequential use of primary malignant circulating prostate cells to detect prostate cancer. Can Cell Microenviron 2015; 2: e714.
This article provides an overview of four radiological accidents in Latin America, and includes a... more This article provides an overview of four radiological accidents in Latin America, and includes a history of the events, the clinical manifestations and health consequences for the exposed individuals, the medical response based on preclinical studies and the role of the International Atomic Energy Agency (IAEA) in coordinating medical response assistance.
We investigated the potential existence of differences in the distribution of T-lymphocyte subset... more We investigated the potential existence of differences in the distribution of T-lymphocyte subsets and in the proliferative response of these CD2+ cells to polyclonal mitogens in patients with transitional cell bladder carcinoma (SBTCC) treated with prophylactic intracavitary instillations of bacillus Calmette-Guérin (BCG) according to their clinical response to this treatment. Before BCG treatment, different subset distribution (CD8+ and CD3+ CD56+), activation antigen expression (CD3+ HLA-DR+) and proliferative response to mitogenic signals were found in CD2+ cells from SBTCC patients prophylactically treated with BCG who remained free of disease or those who had recurrence of tumour. Otherwise, the prophylactic intracavitary BCG instillations in SBTCC patients are associated with a transitory variation of T-lymphocyte subset distribution (CD4 and CD8) and activation antigens expression (CD25).
Corrigendum to "Increased expression of cytokines, soluble cytokine receptors, soluble apoptosis ... more Corrigendum to "Increased expression of cytokines, soluble cytokine receptors, soluble apoptosis ligand and apoptosis in dengue" [Virology 452-453 (2014) 42-51]
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Papers by Eduardo Reyes