International Symposium/Conference on Music Information Retrieval, Aug 24, 2020
A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences ... more A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences in a live setting by a DJ on stage. As a DJ mix is produced in a studio or the live version is recorded for music streaming services, computational methods to analyze DJ mixes, for example, extracting track information or understanding DJ techniques, have drawn research interests. Many of previous works are, however, limited to identifying individual tracks in a mix or segmenting it, and the sizes of the datasets are usually small. In this paper, we provide an in-depth analysis of DJ music by aligning a mix to its original music tracks. We set up the subsequence alignment such that the audio features are less sensitive to the tempo or key change of the original track in a mix. This approach provides temporally tight mix-to-track matching from which we can obtain cue-points, transition length, mix segmentation, and musical changes in DJ performance. Using 1,557 mixes from 1001Tracklists including 13,728 tracks and 20,765 transitions, we conduct the proposed analysis and show a wide range of statistics, which may elucidate the creative process of DJ music making.
A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences ... more A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences in a live setting by a DJ on stage. As a DJ mix is produced in a studio or the live version is recorded for music streaming services, computational methods to analyze DJ mixes, for example, extracting track information or understanding DJ techniques, have drawn research interests. Many of previous works are, however, limited to identifying individual tracks in a mix or segmenting it, and the sizes of the datasets are usually small. In this paper, we provide an in-depth analysis of DJ music by aligning a mix to its original music tracks. We set up the subsequence alignment such that the audio features are less sensitive to the tempo or key change of the original track in a mix. This approach provides temporally tight mix-to-track matching from which we can obtain cue-points, transition length, mix segmentation, and musical changes in DJ performance. Using 1,557 mixes from 1001Tracklists i...
as follows: stage none, 58% (290/503); mild, 42% (128/303); moderate, 25% (36/142); and severe, 1... more as follows: stage none, 58% (290/503); mild, 42% (128/303); moderate, 25% (36/142); and severe, 16% (9/57) (Z 2 = 73.93, p < 0.001). Survival rates by TNM stage were as follows: stage I, 72% (153/213); stage II, 54% (127/234); stage III, 36% (90/249); and stage IV, 30% (93/309) (Z 2 = 100.46, p < 0.001). When symptom severity stage and TNM stage were entered in a proportional-hazards model along with comorbidity, all three variables were independently predictive of survival duration (risk ratio: TNM 1.295, symptom severity 1.311, comorbidity 2.259). Conclusions: Appropriately defined symptoms provide important prognostic information that is independent of TNM and thus provides an index of the biological behavior of head and neck cancer.
Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margi... more Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margin of the right upper eyelid of a 40-year-old man. The clinical diagnoses were either a conjunctival inclusion cyst or an adnexal cyst, possibly of the gland of Moll. The excised specimen was studied histopathologically and exhibited a lining that was virtually indistinguishable from that of the normal canaliculus, except for hyperplasia of the cellular wall and the focal presence of a superficial mucin-producing columnar cell monolayer at the lumen. The medial edge of the excised specimen showed a merging of the cyst into a nonectatic portion of the canaliculus. Inflammation in the wall of the cyst and in this terminal portion of the canaliculus was not identified. This is believed to be a unique entity of an idiopathic ectasia with mild epithelial proliferation of a segment of the canaliculus. The authors have termed this condition canaliculops, to draw a parallel between it and ectasia of the ducts of the lacrimal gland, so-called dacryops.
Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sectio... more Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sections of normal human conjunctiva (epibulbar, tarsal, and forniceal). The overwhelming majority of lymphocytes were T-cells (Leu1+ and OKT8+). In the epithelium, the predominant cell type was the OKT8+ cytotoxic/suppressor cell, whereas in the substantia propria, helper T-cells (Leu3a/3b+) equalled suppressor T-cells. T-cells outnumbered B-cells 20-fold; the letter cells were detected only in the substantial propria, particularly in the fornices, and not in the epithelium. Plasma cells, as identified by OKT10 staining, were completely absent except in the accessory lacrimal glands of Krause. Langerhans&#39; cells, identified by OKT6 and HLA-DR (la) staining, were observed in the epithelium of all conjunctival regions. Our findings suggest that the reactivity of B-lymphocytes and plasma cells is heavily damped down by T-lymphocytes, thus allying the conjunctiva to other mucosal membranes and the skin as heavily immunoregulated tissues.
Survival rates by TNM stage were as follows: stage I, 72% (153/213); stage II, 54% (127/234); sta... more Survival rates by TNM stage were as follows: stage I, 72% (153/213); stage II, 54% (127/234); stage III, 36% (90/249); and stage IV, 30% (93/309) (Z 2 = 100.46, p < 0.001). When symptom severity stage and TNM stage were entered in a proportional-hazards model along with comorbidity, all three variables were independently predictive of survival duration (risk ratio: TNM 1.295, symptom severity 1.311, comorbidity 2.259). Conclusions: Appropriately defined symptoms provide important prognostic information that is independent of TNM and thus provides an index of the biological behavior of head and neck cancer.
Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnorm... more Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnormalities, was studied to determine its possible deleterious effects on the mammalian eye. Young (3.5-week-old) Columbia-Sherman rats were exposed simultaneously to a constant magnetic field of 2.7 tesla and radio frequency pulses of 29 MHz at 800-ms intervals for six hours at field strengths representing the maximum used in a clinical setting. The six-hour exposure is many times greater than the four to six minutes currently employed in most diagnostic protocols. The animals were examined by slit-lamp biomicroscopy and ophthalmoscopy at regular intervals. Autoradiograms of lenses from animals injected with tritiated thymidine prior to exposure did not reveal any disturbances in cell-cycle kinetics. Eyes from rats not previously injected with the isotope were processed for cytopathologic analysis at various intervals. A two-year follow-up has indicated that at both the slit-lamp biomicroscopic and the light microscopic levels, there were no discernable effects on the rat eye.
Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid ... more Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid infiltrates (formerly called reactive lymphoid hyperplasia) of the choroid and contiguous extraocular tissues. There were multifocal, con fluent, and nonconfluent creamy patches in the choroid. These lesions changed very little over periods of observation ranging from 1 to 4 years and failed to produce ophthalmoscopically visible disturbances of the retinal pigment epithe lium (RPE). Fluorescein angiography demonstrated dye collection within the lesions without leakage into the subretinal or subpigment epithelial spaces. Both ultrasonography and computed tomography (CT) showed thickening of the choroid with either anterior or posterior episcleral extensions of lymphoid tissue. Pathologic evaluation of biopsy specimens of extraocular portions of the lesions showed low-grade tumors that were diffuse infiltrates of mature lymphocytes, which exhibited lymphoplasmacytoid features, Dutcher bodies, or small residual germinal centers (so-called borderline lesions). Two patients had uniocular lo calized disease, whereas the third had bilateral ocular lesions, hypogamma globulinemia, and another extranodal chest wall lymphoid tumor. Therapy for the localized ocular condition consisted either of oral administration of corti costeroids or low doses of radiotherapy.
An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 y... more An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 years&#39; duration. The cysts were remarkable for possessing a superior dome of either creamy or yellowish material (probably reflecting the lipid-rich decapitation secretion), surmounting a clearer fluid inferiorly, as well as for being restricted to the eyelid margins where the glands of Moll are located. Light microscopy disclosed a double-layered cuboidal cellular lining, which focally became more plump or hobnailed, exhibited apical decapitation secretion, and was occasionally thrown into papillary folds. Electron microscopy demonstrated cellular debris and segments of interrupted plasmalemmas within the cystic cavity, intercellular widenings constituting canaliculi, cytoplasmic election-dense secretory granules, flocculent mucous vacuoles, and swirls of smooth-surfaced endoplasmic reticulum. Basal cells were focally interspersed between the secretory cells and the basement membrane, and occasionally manifested spotty myofilamentary differentiation. These findings are supportive of an origin of the lesions from the secretory rather than the ductal portion of the glands of Moll. They corroborate conclusions drawn from dermatologic investigations that the cysts are an essentially proliferative process as opposed to the result of passive ductal dilatation.
A 39-year-old black woman had a 12-year history of slowly progressive left temporal juxtalimbal c... more A 39-year-old black woman had a 12-year history of slowly progressive left temporal juxtalimbal conjunctival swelling. The lesion was freely movable over the surface of the globe and was believed to be situated within the substantia propria. Histopathologic evaluation of the excised tissue disclosed that it was a collagenous mass of lamellar architecture, with a hypocellular dispersion of mesenchymal cells and a scattering of capillaries in the absence of inflammation. Electron microscopic examination revealed that the lamellar arrangement had regular lobular subdivisions composed of collagen fibers approximately 50 nm in diameter. Compressed fibroblasts occupied the peripheries of the lobules; the former exhibited abundant rough-surfaced endoplasmic reticulum and failed to elaborate basement membrane material. Delicate processes of the fibroblasts extended into the centers of the collagenous aggregates. To our knowledge, this is the first convincingly documented case of an acquired fibroma of the substantia propria of the epibulbar conjunctiva. The differential diagnosis in this case included related fibroblastic and simulating nonfibroblastic lesions.
Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnorm... more Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnormalities, was studied to determine its possible deleterious effects on the mammalian eye. Young (3.5-week-old) Columbia-Sherman rats were exposed simultaneously to a constant magnetic field of 2.7 tesla and radio frequency pulses of 29 MHz at 800-ms intervals for six hours at field strengths representing the maximum used in a clinical setting. The six-hour exposure is many times greater than the four to six minutes currently employed in most diagnostic protocols. The animals were examined by slit-lamp biomicroscopy and ophthalmoscopy at regular intervals. Autoradiograms of lenses from animals injected with tritiated thymidine prior to exposure did not reveal any disturbances in cell-cycle kinetics. Eyes from rats not previously injected with the isotope were processed for cytopathologic analysis at various intervals. A two-year follow-up has indicated that at both the slit-lamp biomicroscopic and the light microscopic levels, there were no discernable effects on the rat eye.
An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 y... more An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 years&#39; duration. The cysts were remarkable for possessing a superior dome of either creamy or yellowish material (probably reflecting the lipid-rich decapitation secretion), surmounting a clearer fluid inferiorly, as well as for being restricted to the eyelid margins where the glands of Moll are located. Light microscopy disclosed a double-layered cuboidal cellular lining, which focally became more plump or hobnailed, exhibited apical decapitation secretion, and was occasionally thrown into papillary folds. Electron microscopy demonstrated cellular debris and segments of interrupted plasmalemmas within the cystic cavity, intercellular widenings constituting canaliculi, cytoplasmic election-dense secretory granules, flocculent mucous vacuoles, and swirls of smooth-surfaced endoplasmic reticulum. Basal cells were focally interspersed between the secretory cells and the basement membrane, and occasionally manifested spotty myofilamentary differentiation. These findings are supportive of an origin of the lesions from the secretory rather than the ductal portion of the glands of Moll. They corroborate conclusions drawn from dermatologic investigations that the cysts are an essentially proliferative process as opposed to the result of passive ductal dilatation.
Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margi... more Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margin of the right upper eyelid of a 40-year-old man. The clinical diagnoses were either a conjunctival inclusion cyst or an adnexal cyst, possibly of the gland of Moll. The excised specimen was studied histopathologically and exhibited a lining that was virtually indistinguishable from that of the normal canaliculus, except for hyperplasia of the cellular wall and the focal presence of a superficial mucin-producing columnar cell monolayer at the lumen. The medial edge of the excised specimen showed a merging of the cyst into a nonectatic portion of the canaliculus. Inflammation in the wall of the cyst and in this terminal portion of the canaliculus was not identified. This is believed to be a unique entity of an idiopathic ectasia with mild epithelial proliferation of a segment of the canaliculus. The authors have termed this condition canaliculops, to draw a parallel between it and ectasia of the ducts of the lacrimal gland, so-called dacryops.
Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sectio... more Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sections of normal human conjunctiva (epibulbar, tarsal, and forniceal). The overwhelming majority of lymphocytes were T-cells (Leu1+ and OKT8+). In the epithelium, the predominant cell type was the OKT8+ cytotoxic/suppressor cell, whereas in the substantia propria, helper T-cells (Leu3a/3b+) equalled suppressor T-cells. T-cells outnumbered B-cells 20-fold; the letter cells were detected only in the substantial propria, particularly in the fornices, and not in the epithelium. Plasma cells, as identified by OKT10 staining, were completely absent except in the accessory lacrimal glands of Krause. Langerhans&#39; cells, identified by OKT6 and HLA-DR (la) staining, were observed in the epithelium of all conjunctival regions. Our findings suggest that the reactivity of B-lymphocytes and plasma cells is heavily damped down by T-lymphocytes, thus allying the conjunctiva to other mucosal membranes and the skin as heavily immunoregulated tissues.
Langerhans cells belong to the dendritic cell family. Their presence in the conjunctiva and corne... more Langerhans cells belong to the dendritic cell family. Their presence in the conjunctiva and cornea has been demonstrated by means of various membrane and cytoplasmic markers. Utilizing OKT6, a monoclonal antibody that specifically binds to Langerhans cells in conjunction with la histocompatibility antigens (HLA-DR), and a new panel of monoclonal antibodies, we compared the population density and characteristic phenotypes of Langerhans cells in normal conjunctiva with those in normal epidermis. A greater density of Langerhans cells was noted in epidermis in comparison with conjunctiva. Various areas of the conjunctiva and cornea were mapped for Langerhans cell distribution. The T6/la ratio of Langerhans cells in conjunctiva was notably different from that in skin. Utilizing the Prolm2 marker, we identified non-Langerhans dendritic cells in the substantia propria and in the basilar epithelium of the conjunctiva, antigen-processing cells probably identical to the interdigitating dendritic cells of lymph nodes.
To delineate the immunoarchitecture of the normal human lacrimal gland, monoclonal antibodies tha... more To delineate the immunoarchitecture of the normal human lacrimal gland, monoclonal antibodies that detect B- and T-lymphocyte, macrophage, and dendritic cell lineage, subset, and differentiation-associated antigens were used in combination with highly sensitive tissue-section immunoperoxidase techniques. Plasma cells, identified with monoclonal antibody OKT10, represented the predominant mononuclear cell population, accounting for 53.9% of all mononuclear cells present. A qualitative study of plasma cell cytoplasmic immunoglobulin heavy-chain expression in deparaffinized, formalin-fixed tissue sections showed that the vast majority of these plasma cells contained IgA. Rare plasma cells contained IgG, IgM, or IgD. T-cells, identified with monoclonal antibody OKT3, represented the second most common cell type in the normal human lacrimal gland, accounting for 40.3% of all mononuclear cells present. T cells were located predominantly in lymphocytic foci and singly in the interstitium. T8 antigen-positive (suppressor/cytotoxic) T cells predominated over T4 antigen-positive (helper) T cells, averaging 25.2 and 14.7%, respectively. The overall mean T4/T8 ratio was 0.56. T8 antigen-positive T cells were the most numerous cell population outside aggregates and follicles, being distributed almost equally between the acini and the ducts (49%) and the interstitium (51%). Only 16% of T4 antigen-positive cells preferred glands or ducts to the interstitium. B cells, identified with monoclonal antibody BL9, represented only 5.7% of all mononuclear cells present. B cells were exclusively found in the centers of primary and secondary follicles. The majority of the surface immunoglobulin-positive B cells expressed IgM, fewer expressed IgD, and still fewer expressed IgG or IgA. Rare LeuM1+ OKM1+ macrophages were present in the center of B-cell follicles, where rare OKT6+ dendritic cells and activated T cells (IL-2+) were also discovered. These results support the concept that the lacrimal gland belongs to the mucosa-associated lymphoid system.
Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid ... more Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid infiltrates (formerly called reactive lymphoid hyperplasia) of the choroid and contiguous extraocular tissues. There were multifocal, con fluent, and nonconfluent creamy patches in the choroid. These lesions changed very little over periods of observation ranging from 1 to 4 years and failed to produce ophthalmoscopically visible disturbances of the retinal pigment epithe lium (RPE). Fluorescein angiography demonstrated dye collection within the lesions without leakage into the subretinal or subpigment epithelial spaces. Both ultrasonography and computed tomography (CT) showed thickening of the choroid with either anterior or posterior episcleral extensions of lymphoid tissue. Pathologic evaluation of biopsy specimens of extraocular portions of the lesions showed low-grade tumors that were diffuse infiltrates of mature lymphocytes, which exhibited lymphoplasmacytoid features, Dutcher bodies, or small residual germinal centers (so-called borderline lesions). Two patients had uniocular lo calized disease, whereas the third had bilateral ocular lesions, hypogamma globulinemia, and another extranodal chest wall lymphoid tumor. Therapy for the localized ocular condition consisted either of oral administration of corti costeroids or low doses of radiotherapy.
Ocular cicatricial pemphigoid (OCP) is characterized by the deposition of immunoglobulin and comp... more Ocular cicatricial pemphigoid (OCP) is characterized by the deposition of immunoglobulin and complement along the conjunctival epithelial basement membrane zone (BMZ). In order to further elucidate the cellular populations of the local inflammatory infiltrates, the authors used a panel of monoclonal antibodies in cryostat tissue sections to delineate T cell subsets, B lymphocytes, dendritic cells, and macrophages in six patients with OCP. In comparison with matched controls of the epibulbar conjunctiva, the authors discovered a threefold increase in T lymphocytes within the epithelium and a 20-fold increase within the substantia propria. In contrast with the normal-standing population of conjunctival T lymphocytes, there were activated interleukin 2 receptor (IL-2R)-positive lymphocytes in both the epithelium and the substantia propria. Macrophages were the second most common cells in the substantia propria, accounting for 12.7% of the mononuclear population-a threefold increase over the normal percentage. B cells and plasma cells, normally absent from epibulbar conjunctiva, were the next most prominent populations, constituting 6.9 and 4.6%, respectively, of all mononuclear cells. Dendritic cells which process antigen locally constituted only 1.2% of the mononuclear cell population, but were increased 25-fold over normal controls. By elaborating cytokines that promote fibroplasia, the T cells in OCP may be effector cells along with macrophages and other inflammatory cells in bringing about scarification of the substantia propria, and may furthermore be responsible for an immunoregulatory defect that allows local B lymphocytes to produce autoantibodies to the BMZ. Ophthalmology 96: 236-243, 1989
International Symposium/Conference on Music Information Retrieval, Aug 24, 2020
A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences ... more A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences in a live setting by a DJ on stage. As a DJ mix is produced in a studio or the live version is recorded for music streaming services, computational methods to analyze DJ mixes, for example, extracting track information or understanding DJ techniques, have drawn research interests. Many of previous works are, however, limited to identifying individual tracks in a mix or segmenting it, and the sizes of the datasets are usually small. In this paper, we provide an in-depth analysis of DJ music by aligning a mix to its original music tracks. We set up the subsequence alignment such that the audio features are less sensitive to the tempo or key change of the original track in a mix. This approach provides temporally tight mix-to-track matching from which we can obtain cue-points, transition length, mix segmentation, and musical changes in DJ performance. Using 1,557 mixes from 1001Tracklists including 13,728 tracks and 20,765 transitions, we conduct the proposed analysis and show a wide range of statistics, which may elucidate the creative process of DJ music making.
A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences ... more A DJ mix is a sequence of music tracks concatenated seamlessly, typically rendered for audiences in a live setting by a DJ on stage. As a DJ mix is produced in a studio or the live version is recorded for music streaming services, computational methods to analyze DJ mixes, for example, extracting track information or understanding DJ techniques, have drawn research interests. Many of previous works are, however, limited to identifying individual tracks in a mix or segmenting it, and the sizes of the datasets are usually small. In this paper, we provide an in-depth analysis of DJ music by aligning a mix to its original music tracks. We set up the subsequence alignment such that the audio features are less sensitive to the tempo or key change of the original track in a mix. This approach provides temporally tight mix-to-track matching from which we can obtain cue-points, transition length, mix segmentation, and musical changes in DJ performance. Using 1,557 mixes from 1001Tracklists i...
as follows: stage none, 58% (290/503); mild, 42% (128/303); moderate, 25% (36/142); and severe, 1... more as follows: stage none, 58% (290/503); mild, 42% (128/303); moderate, 25% (36/142); and severe, 16% (9/57) (Z 2 = 73.93, p < 0.001). Survival rates by TNM stage were as follows: stage I, 72% (153/213); stage II, 54% (127/234); stage III, 36% (90/249); and stage IV, 30% (93/309) (Z 2 = 100.46, p < 0.001). When symptom severity stage and TNM stage were entered in a proportional-hazards model along with comorbidity, all three variables were independently predictive of survival duration (risk ratio: TNM 1.295, symptom severity 1.311, comorbidity 2.259). Conclusions: Appropriately defined symptoms provide important prognostic information that is independent of TNM and thus provides an index of the biological behavior of head and neck cancer.
Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margi... more Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margin of the right upper eyelid of a 40-year-old man. The clinical diagnoses were either a conjunctival inclusion cyst or an adnexal cyst, possibly of the gland of Moll. The excised specimen was studied histopathologically and exhibited a lining that was virtually indistinguishable from that of the normal canaliculus, except for hyperplasia of the cellular wall and the focal presence of a superficial mucin-producing columnar cell monolayer at the lumen. The medial edge of the excised specimen showed a merging of the cyst into a nonectatic portion of the canaliculus. Inflammation in the wall of the cyst and in this terminal portion of the canaliculus was not identified. This is believed to be a unique entity of an idiopathic ectasia with mild epithelial proliferation of a segment of the canaliculus. The authors have termed this condition canaliculops, to draw a parallel between it and ectasia of the ducts of the lacrimal gland, so-called dacryops.
Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sectio... more Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sections of normal human conjunctiva (epibulbar, tarsal, and forniceal). The overwhelming majority of lymphocytes were T-cells (Leu1+ and OKT8+). In the epithelium, the predominant cell type was the OKT8+ cytotoxic/suppressor cell, whereas in the substantia propria, helper T-cells (Leu3a/3b+) equalled suppressor T-cells. T-cells outnumbered B-cells 20-fold; the letter cells were detected only in the substantial propria, particularly in the fornices, and not in the epithelium. Plasma cells, as identified by OKT10 staining, were completely absent except in the accessory lacrimal glands of Krause. Langerhans&#39; cells, identified by OKT6 and HLA-DR (la) staining, were observed in the epithelium of all conjunctival regions. Our findings suggest that the reactivity of B-lymphocytes and plasma cells is heavily damped down by T-lymphocytes, thus allying the conjunctiva to other mucosal membranes and the skin as heavily immunoregulated tissues.
Survival rates by TNM stage were as follows: stage I, 72% (153/213); stage II, 54% (127/234); sta... more Survival rates by TNM stage were as follows: stage I, 72% (153/213); stage II, 54% (127/234); stage III, 36% (90/249); and stage IV, 30% (93/309) (Z 2 = 100.46, p < 0.001). When symptom severity stage and TNM stage were entered in a proportional-hazards model along with comorbidity, all three variables were independently predictive of survival duration (risk ratio: TNM 1.295, symptom severity 1.311, comorbidity 2.259). Conclusions: Appropriately defined symptoms provide important prognostic information that is independent of TNM and thus provides an index of the biological behavior of head and neck cancer.
Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnorm... more Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnormalities, was studied to determine its possible deleterious effects on the mammalian eye. Young (3.5-week-old) Columbia-Sherman rats were exposed simultaneously to a constant magnetic field of 2.7 tesla and radio frequency pulses of 29 MHz at 800-ms intervals for six hours at field strengths representing the maximum used in a clinical setting. The six-hour exposure is many times greater than the four to six minutes currently employed in most diagnostic protocols. The animals were examined by slit-lamp biomicroscopy and ophthalmoscopy at regular intervals. Autoradiograms of lenses from animals injected with tritiated thymidine prior to exposure did not reveal any disturbances in cell-cycle kinetics. Eyes from rats not previously injected with the isotope were processed for cytopathologic analysis at various intervals. A two-year follow-up has indicated that at both the slit-lamp biomicroscopic and the light microscopic levels, there were no discernable effects on the rat eye.
Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid ... more Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid infiltrates (formerly called reactive lymphoid hyperplasia) of the choroid and contiguous extraocular tissues. There were multifocal, con fluent, and nonconfluent creamy patches in the choroid. These lesions changed very little over periods of observation ranging from 1 to 4 years and failed to produce ophthalmoscopically visible disturbances of the retinal pigment epithe lium (RPE). Fluorescein angiography demonstrated dye collection within the lesions without leakage into the subretinal or subpigment epithelial spaces. Both ultrasonography and computed tomography (CT) showed thickening of the choroid with either anterior or posterior episcleral extensions of lymphoid tissue. Pathologic evaluation of biopsy specimens of extraocular portions of the lesions showed low-grade tumors that were diffuse infiltrates of mature lymphocytes, which exhibited lymphoplasmacytoid features, Dutcher bodies, or small residual germinal centers (so-called borderline lesions). Two patients had uniocular lo calized disease, whereas the third had bilateral ocular lesions, hypogamma globulinemia, and another extranodal chest wall lymphoid tumor. Therapy for the localized ocular condition consisted either of oral administration of corti costeroids or low doses of radiotherapy.
An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 y... more An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 years&#39; duration. The cysts were remarkable for possessing a superior dome of either creamy or yellowish material (probably reflecting the lipid-rich decapitation secretion), surmounting a clearer fluid inferiorly, as well as for being restricted to the eyelid margins where the glands of Moll are located. Light microscopy disclosed a double-layered cuboidal cellular lining, which focally became more plump or hobnailed, exhibited apical decapitation secretion, and was occasionally thrown into papillary folds. Electron microscopy demonstrated cellular debris and segments of interrupted plasmalemmas within the cystic cavity, intercellular widenings constituting canaliculi, cytoplasmic election-dense secretory granules, flocculent mucous vacuoles, and swirls of smooth-surfaced endoplasmic reticulum. Basal cells were focally interspersed between the secretory cells and the basement membrane, and occasionally manifested spotty myofilamentary differentiation. These findings are supportive of an origin of the lesions from the secretory rather than the ductal portion of the glands of Moll. They corroborate conclusions drawn from dermatologic investigations that the cysts are an essentially proliferative process as opposed to the result of passive ductal dilatation.
A 39-year-old black woman had a 12-year history of slowly progressive left temporal juxtalimbal c... more A 39-year-old black woman had a 12-year history of slowly progressive left temporal juxtalimbal conjunctival swelling. The lesion was freely movable over the surface of the globe and was believed to be situated within the substantia propria. Histopathologic evaluation of the excised tissue disclosed that it was a collagenous mass of lamellar architecture, with a hypocellular dispersion of mesenchymal cells and a scattering of capillaries in the absence of inflammation. Electron microscopic examination revealed that the lamellar arrangement had regular lobular subdivisions composed of collagen fibers approximately 50 nm in diameter. Compressed fibroblasts occupied the peripheries of the lobules; the former exhibited abundant rough-surfaced endoplasmic reticulum and failed to elaborate basement membrane material. Delicate processes of the fibroblasts extended into the centers of the collagenous aggregates. To our knowledge, this is the first convincingly documented case of an acquired fibroma of the substantia propria of the epibulbar conjunctiva. The differential diagnosis in this case included related fibroblastic and simulating nonfibroblastic lesions.
Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnorm... more Nuclear magnetic resonance, an imaging technique with great promise for detecting cerebral abnormalities, was studied to determine its possible deleterious effects on the mammalian eye. Young (3.5-week-old) Columbia-Sherman rats were exposed simultaneously to a constant magnetic field of 2.7 tesla and radio frequency pulses of 29 MHz at 800-ms intervals for six hours at field strengths representing the maximum used in a clinical setting. The six-hour exposure is many times greater than the four to six minutes currently employed in most diagnostic protocols. The animals were examined by slit-lamp biomicroscopy and ophthalmoscopy at regular intervals. Autoradiograms of lenses from animals injected with tritiated thymidine prior to exposure did not reveal any disturbances in cell-cycle kinetics. Eyes from rats not previously injected with the isotope were processed for cytopathologic analysis at various intervals. A two-year follow-up has indicated that at both the slit-lamp biomicroscopic and the light microscopic levels, there were no discernable effects on the rat eye.
An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 y... more An 88-year-old man presented with 13 apocrine cystadenomas of both lower eyelids of at least 10 years&#39; duration. The cysts were remarkable for possessing a superior dome of either creamy or yellowish material (probably reflecting the lipid-rich decapitation secretion), surmounting a clearer fluid inferiorly, as well as for being restricted to the eyelid margins where the glands of Moll are located. Light microscopy disclosed a double-layered cuboidal cellular lining, which focally became more plump or hobnailed, exhibited apical decapitation secretion, and was occasionally thrown into papillary folds. Electron microscopy demonstrated cellular debris and segments of interrupted plasmalemmas within the cystic cavity, intercellular widenings constituting canaliculi, cytoplasmic election-dense secretory granules, flocculent mucous vacuoles, and swirls of smooth-surfaced endoplasmic reticulum. Basal cells were focally interspersed between the secretory cells and the basement membrane, and occasionally manifested spotty myofilamentary differentiation. These findings are supportive of an origin of the lesions from the secretory rather than the ductal portion of the glands of Moll. They corroborate conclusions drawn from dermatologic investigations that the cysts are an essentially proliferative process as opposed to the result of passive ductal dilatation.
Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margi... more Over a 1-year period, a cystic bluish coloration developed in the most medial aspect at the margin of the right upper eyelid of a 40-year-old man. The clinical diagnoses were either a conjunctival inclusion cyst or an adnexal cyst, possibly of the gland of Moll. The excised specimen was studied histopathologically and exhibited a lining that was virtually indistinguishable from that of the normal canaliculus, except for hyperplasia of the cellular wall and the focal presence of a superficial mucin-producing columnar cell monolayer at the lumen. The medial edge of the excised specimen showed a merging of the cyst into a nonectatic portion of the canaliculus. Inflammation in the wall of the cyst and in this terminal portion of the canaliculus was not identified. This is believed to be a unique entity of an idiopathic ectasia with mild epithelial proliferation of a segment of the canaliculus. The authors have termed this condition canaliculops, to draw a parallel between it and ectasia of the ducts of the lacrimal gland, so-called dacryops.
Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sectio... more Monoclonal antibodies were used to analyze the lymphocytic subpopulations in frozen tissue sections of normal human conjunctiva (epibulbar, tarsal, and forniceal). The overwhelming majority of lymphocytes were T-cells (Leu1+ and OKT8+). In the epithelium, the predominant cell type was the OKT8+ cytotoxic/suppressor cell, whereas in the substantia propria, helper T-cells (Leu3a/3b+) equalled suppressor T-cells. T-cells outnumbered B-cells 20-fold; the letter cells were detected only in the substantial propria, particularly in the fornices, and not in the epithelium. Plasma cells, as identified by OKT10 staining, were completely absent except in the accessory lacrimal glands of Krause. Langerhans&#39; cells, identified by OKT6 and HLA-DR (la) staining, were observed in the epithelium of all conjunctival regions. Our findings suggest that the reactivity of B-lymphocytes and plasma cells is heavily damped down by T-lymphocytes, thus allying the conjunctiva to other mucosal membranes and the skin as heavily immunoregulated tissues.
Langerhans cells belong to the dendritic cell family. Their presence in the conjunctiva and corne... more Langerhans cells belong to the dendritic cell family. Their presence in the conjunctiva and cornea has been demonstrated by means of various membrane and cytoplasmic markers. Utilizing OKT6, a monoclonal antibody that specifically binds to Langerhans cells in conjunction with la histocompatibility antigens (HLA-DR), and a new panel of monoclonal antibodies, we compared the population density and characteristic phenotypes of Langerhans cells in normal conjunctiva with those in normal epidermis. A greater density of Langerhans cells was noted in epidermis in comparison with conjunctiva. Various areas of the conjunctiva and cornea were mapped for Langerhans cell distribution. The T6/la ratio of Langerhans cells in conjunctiva was notably different from that in skin. Utilizing the Prolm2 marker, we identified non-Langerhans dendritic cells in the substantia propria and in the basilar epithelium of the conjunctiva, antigen-processing cells probably identical to the interdigitating dendritic cells of lymph nodes.
To delineate the immunoarchitecture of the normal human lacrimal gland, monoclonal antibodies tha... more To delineate the immunoarchitecture of the normal human lacrimal gland, monoclonal antibodies that detect B- and T-lymphocyte, macrophage, and dendritic cell lineage, subset, and differentiation-associated antigens were used in combination with highly sensitive tissue-section immunoperoxidase techniques. Plasma cells, identified with monoclonal antibody OKT10, represented the predominant mononuclear cell population, accounting for 53.9% of all mononuclear cells present. A qualitative study of plasma cell cytoplasmic immunoglobulin heavy-chain expression in deparaffinized, formalin-fixed tissue sections showed that the vast majority of these plasma cells contained IgA. Rare plasma cells contained IgG, IgM, or IgD. T-cells, identified with monoclonal antibody OKT3, represented the second most common cell type in the normal human lacrimal gland, accounting for 40.3% of all mononuclear cells present. T cells were located predominantly in lymphocytic foci and singly in the interstitium. T8 antigen-positive (suppressor/cytotoxic) T cells predominated over T4 antigen-positive (helper) T cells, averaging 25.2 and 14.7%, respectively. The overall mean T4/T8 ratio was 0.56. T8 antigen-positive T cells were the most numerous cell population outside aggregates and follicles, being distributed almost equally between the acini and the ducts (49%) and the interstitium (51%). Only 16% of T4 antigen-positive cells preferred glands or ducts to the interstitium. B cells, identified with monoclonal antibody BL9, represented only 5.7% of all mononuclear cells present. B cells were exclusively found in the centers of primary and secondary follicles. The majority of the surface immunoglobulin-positive B cells expressed IgM, fewer expressed IgD, and still fewer expressed IgG or IgA. Rare LeuM1+ OKM1+ macrophages were present in the center of B-cell follicles, where rare OKT6+ dendritic cells and activated T cells (IL-2+) were also discovered. These results support the concept that the lacrimal gland belongs to the mucosa-associated lymphoid system.
Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid ... more Three patients, each more than 60 years of age at initial presentation, had early stage lymphoid infiltrates (formerly called reactive lymphoid hyperplasia) of the choroid and contiguous extraocular tissues. There were multifocal, con fluent, and nonconfluent creamy patches in the choroid. These lesions changed very little over periods of observation ranging from 1 to 4 years and failed to produce ophthalmoscopically visible disturbances of the retinal pigment epithe lium (RPE). Fluorescein angiography demonstrated dye collection within the lesions without leakage into the subretinal or subpigment epithelial spaces. Both ultrasonography and computed tomography (CT) showed thickening of the choroid with either anterior or posterior episcleral extensions of lymphoid tissue. Pathologic evaluation of biopsy specimens of extraocular portions of the lesions showed low-grade tumors that were diffuse infiltrates of mature lymphocytes, which exhibited lymphoplasmacytoid features, Dutcher bodies, or small residual germinal centers (so-called borderline lesions). Two patients had uniocular lo calized disease, whereas the third had bilateral ocular lesions, hypogamma globulinemia, and another extranodal chest wall lymphoid tumor. Therapy for the localized ocular condition consisted either of oral administration of corti costeroids or low doses of radiotherapy.
Ocular cicatricial pemphigoid (OCP) is characterized by the deposition of immunoglobulin and comp... more Ocular cicatricial pemphigoid (OCP) is characterized by the deposition of immunoglobulin and complement along the conjunctival epithelial basement membrane zone (BMZ). In order to further elucidate the cellular populations of the local inflammatory infiltrates, the authors used a panel of monoclonal antibodies in cryostat tissue sections to delineate T cell subsets, B lymphocytes, dendritic cells, and macrophages in six patients with OCP. In comparison with matched controls of the epibulbar conjunctiva, the authors discovered a threefold increase in T lymphocytes within the epithelium and a 20-fold increase within the substantia propria. In contrast with the normal-standing population of conjunctival T lymphocytes, there were activated interleukin 2 receptor (IL-2R)-positive lymphocytes in both the epithelium and the substantia propria. Macrophages were the second most common cells in the substantia propria, accounting for 12.7% of the mononuclear population-a threefold increase over the normal percentage. B cells and plasma cells, normally absent from epibulbar conjunctiva, were the next most prominent populations, constituting 6.9 and 4.6%, respectively, of all mononuclear cells. Dendritic cells which process antigen locally constituted only 1.2% of the mononuclear cell population, but were increased 25-fold over normal controls. By elaborating cytokines that promote fibroplasia, the T cells in OCP may be effector cells along with macrophages and other inflammatory cells in bringing about scarification of the substantia propria, and may furthermore be responsible for an immunoregulatory defect that allows local B lymphocytes to produce autoantibodies to the BMZ. Ophthalmology 96: 236-243, 1989
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Papers by Evan Sacks