Papers by Federico Navarro-Garcia
International Orthopaedics, 2022
Purpose Microencapsulation techniques have allowed the addition of rifampicin to bone cement, but... more Purpose Microencapsulation techniques have allowed the addition of rifampicin to bone cement, but its in vivo efficacy has not been proven. The aim of our study is to determine the superiority of cement containing gentamicin and rifampicin microcapsules in the treatment of PJI versus cement exclusively containing gentamicin. Methods An S. aureus PJI was induced in 15 NZW rabbits. A week after inoculation, the first stage of replacement was carried out, and the animals were divided into two groups: group R received a spacer containing gentamicin and rifampicin microcapsules, and group C received a spacer containing gentamicin. Intra-articular release curve of rifampicin and infection and toxicity markers were monitored for four weeks post-operatively, when microbiological analysis was performed. Results The microbiological cultures showed a significantly lower growth of S. aureus in soft tissue (2.3·104 vs 0; p = 0.01) and bone (5.7·102 vs 0; p = 0.03) in the group with rifampicin mi...
Revista de Salud Ambiental, Dec 15, 2015
Uso de lodos de depuradora en agricultura: patógenos y resistencias a antibióticos The agricultur... more Uso de lodos de depuradora en agricultura: patógenos y resistencias a antibióticos The agricultural use of water treatment plant sludge: pathogens and antibiotic resistance Uso de lamas de estações de tratamento na agricultura: patogénicos e resistência a antibióticos
International Journal of Biological Macromolecules, Apr 1, 2023
Knee, Jun 1, 2020
Background: Infection after arthroplasty (prosthetic joint infection; PJI) is a devastating compl... more Background: Infection after arthroplasty (prosthetic joint infection; PJI) is a devastating complication that can lead to functional loss of the affected limb. The purpose of the present study is to develop an animal model of PJI using a three-dimensional printed species-specific implant, which is a step forward for future research to develop new therapeutic strategies. Methods: Fifteen New Zealand White rabbits were employed to reproduce PJI by intra-articular inoculation of 10 5 cfu/ml of Staphylococcus aureus ATCC® 29213. Three-dimensional printing technology was used to design a species-specific four-millimeter-thick implant maintaining the anatomical irregularities of the tibial-articular surface. Response to bacterial inoculation was monitored by clinical (weight and temperature), hematological (leukocyte, lymphocyte and platelet counts) and biochemical (erythrocyte sedimentation rate) analyses at the time of inoculation and seven days thereafter, when microbiological samples for culture were also taken. Results: All animals recovered from surgery and all displayed full weight-bearing four days postoperatively. Fourteen of the 15 tested animals (93.3%) presented positive microbiological cultures. A statistically significant increase was found in the number of platelets and leukocytes, as well as a significant decrease in the percentage of lymphocytes, with P = 0.0001 in all cases. Conclusions: An experimental model faithfully reproducing the periprosthetic infection environment and achieving a high rate of infection has been designed. The use of threedimensional printed species-specific implants allows rapid postoperative recovery of animals and the development of a stable biofilm. These characteristics make it an interesting model to study its pathogenesis and possible therapeutic strategies.
Plant and Cell Physiology, Jul 6, 2018
The presence of antibiotics in soils could be due to natural production by soil microorganisms or... more The presence of antibiotics in soils could be due to natural production by soil microorganisms or to the effect of anthropogenic activities. However, the impact of these compounds on plant physiology has not been thoroughly investigated. To evaluate the effect of b-lactam antibiotics (carbenicillin and penicillin) on the growth and development of Arabidopsis thaliana roots, plants were grown in the presence of different amounts and we found a reduction in root size, an increase in the size of root hairs as well as an abnormal position closer to the tip of the roots. Those phenomena were dependent on the accumulation of both antibiotics inside root tissues and also correlated with a decrease in size of the root apical meristem not related to an alteration in cell division but to a decrease in cell expansion. Using an RNA sequencing analysis, we detected an increase in the expression of genes related to the response to oxidative stress, which would explain the increase in the levels of endogenous reactive oxygen species found in the presence of those antibiotics. Moreover, some auxin-responsive genes were misregulated, especially an induction of CYP79B3, possibly explaining the increase in auxin levels in the presence of carbenicillin and the decrease in the amount of indole glucosinolates, involved in the control of fungal infections. Accordingly, penicillin-treated plants were hypersensitive to the endophyte fungus Colletotrichum tofieldiae. These results underscore the risks for plant growth of b-lactam antibiotics in agricultural soils, and suggest a possible function for these compounds as fungus-produced signaling molecules to modify plant behavior.
Yeast, Dec 1, 1996
PLA, J., GIL, C., MONTEOLIVA, L., NAVARRO-GARCÍA, F., SÁNCHEZ, M. and NOMBELA, C.(1996), Understa... more PLA, J., GIL, C., MONTEOLIVA, L., NAVARRO-GARCÍA, F., SÁNCHEZ, M. and NOMBELA, C.(1996), Understanding Candida albicans at the Molecular Level. Yeast, 12: 1677–1702. doi: 10.1002/(SICI) 1097-0061 (199612) 12: 16< 1677:: AID-YEA79> 3.0. CO; 2-U
Fems Microbiology Reviews, Apr 1, 2001
In recent years, the incidence of fungal infections has been rising all over the world. Although ... more In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for the identification of reliable determinants of virulence. In this review, we focus on identified Candida albicans genes whose deletant strains have been tested in experimental virulence assays. We discuss the putative relationship of these genes to virulence and also outline the use of new different systems to examine the precise effect in virulence of different genes.
Infection and Immunity, Feb 1, 1997
Deletion of the Candida albicans mitogen-activated protein kinase MKC1 gene gave rise to viable c... more Deletion of the Candida albicans mitogen-activated protein kinase MKC1 gene gave rise to viable cells whose cell integrity was affected (F. Navarro-García, M.
DOAJ (DOAJ: Directory of Open Access Journals), Dec 1, 2015
Uso de lodos de depuradora en agricultura: patógenos y resistencias a antibióticos The agricultur... more Uso de lodos de depuradora en agricultura: patógenos y resistencias a antibióticos The agricultural use of water treatment plant sludge: pathogens and antibiotic resistance Uso de lamas de estações de tratamento na agricultura: patogénicos e resistência a antibióticos
Journal of Bacteriology, May 15, 1999
The relevance of the mitogen-activated protein (MAP) kinase Hog1p in Candida albicans was address... more The relevance of the mitogen-activated protein (MAP) kinase Hog1p in Candida albicans was addressed through the characterization of C. albicans strains without a functional HOG1 gene. Analysis of the phenotype of hog1 mutants under osmostressing conditions revealed that this mutant displays a set of morphological alterations as the result of a failure to complete the final stages of cytokinesis, with parallel defects in the budding pattern. Even under permissive conditions, hog1 mutants displayed a different susceptibility to some compounds such as nikkomycin Z or Congo red, which interfere with cell wall functionality. In addition, the hog1 mutant displayed a colony morphology different from that of the wild-type strain on some media which promote morphological transitions in C. albicans. We show that C. albicans hog1 mutants are derepressed in the serum-induced hyphal formation and, consistently with this behavior, that HOG1 overexpression in Saccharomyces cerevisiae represses the pseudodimorphic transition. Most interestingly, deletion of HOG1 resulted in a drastic increase in the mean survival time of systemically infected mice, supporting a role for this MAP kinase pathway in virulence of pathogenic fungi. This finding has potential implications in antifungal therapy.
EGU General Assembly Conference Abstracts, Apr 1, 2019
Orthopaedic Proceedings, Dec 2, 2019
Pharmaceuticals, 2021
Accumulation of cystine crystals in the cornea of patients suffering from cystinosis is considere... more Accumulation of cystine crystals in the cornea of patients suffering from cystinosis is considered pathognomonic and can lead to severe ocular complications. Cysteamine eye drop compounded formulations, commonly prepared by hospital pharmacy services, are meant to diminish the build-up of corneal cystine crystals. The objective of this work was to analyze whether the shelf life proposed for six formulations prepared following different protocols used in hospital pharmacies is adequate to guarantee the quality and efficacy of cysteamine eye drops. The long-term and in-use stabilities of these preparations were studied using different parameters: content of cysteamine and its main degradation product cystamine; appearance, color and odor; pH and viscosity; and microbiological analysis. The results obtained show that degradation of cysteamine was between 20% and 50% after one month of storage in the long-term stability study and between 35% and 60% in the in-use study. These data confi...
Revista de Salud Ambiental, 2015
The use of water treatment plant sludge to restore degraded soils is customary agricultural pract... more The use of water treatment plant sludge to restore degraded soils is customary agricultural practice, but it could be dangerous from the point of view of both health and the environment. A transient increase of either pathogenic or indicator microbial populations, whose persistence in time is variable and attributed to the characteristics of the soil (types of materials in the soil), any amendments (origin and treatments it has undergone) or the weather (humidity and temperature mainly), has often been detected in soils treated with this kind of waste. Given their origin, water treatment plant sludges could lead to the transmission of a) pathogens and b) antibiotic-resistant microorganisms to human beings through the food chain and cause the spreading of antibiotic resistances as a result of their increase and persistence in the soil for variable periods of time. However, Spanish legislation regulating the use of sludges in the farming industry is based on a very restricted microbio...
European Journal of Orthopaedic Surgery & Traumatology, 2019
Introduction Periprosthetic infection is considered an increasing incidence pathology whose thera... more Introduction Periprosthetic infection is considered an increasing incidence pathology whose therapeutic strategies can be defined as unsatisfactory. Currently, animal models are employed to study its physiopathology and strategic therapies, but non-species-specific materials are implanted as foreign bodies. The use of these implants implies intrinsic instability, which hinders the development of a biofilm on their surfaces and complicates the post-operative recovery of the animal. The objective of the present study is the design of a species-specific implant for the New Zealand white (NZW) rabbit by means of 3D printing. Materials and methods A CT scan of the knee of a NZW rabbit was performed, and the tibial surface was reconstructed in order to fabricate a species-specific tibial plateau using Horos ® and Autodesk ® Meshmixer™ software. This implant was inserted in fifteen NZW rabbits, and the assessment of its stability was based on the position of the limb at rest and the animal weight-bearing capacity. Biofilm formation on the surface was demonstrated by crystal violet staining. Results A 1.81 cm × 1 cm × 1.24 cm stainless steel implant was designed. It consisted of a 4-mm-thick tibial plate with a rough surface and an eccentric metaphyseal anchoring. All of the animals exhibited hyperflexion of the operated limb immediately post-operative, and 100% could apply full weight bearing from day 5 after surgery. Conclusions The species-specific design of implants in experimental surgery encourages rapid recovery of the animal and the development of a biofilm on their surfaces, making them ideal for the study of the physiopathology and for establishing possible therapeutic targets for prosthetic infection.
Carbohydrate polymers, 2016
Science of The Total Environment, 2016
Effect of biosolids on fecal and ampicillinresistant bacteria (AmpR) in crop soil • Bacteria in s... more Effect of biosolids on fecal and ampicillinresistant bacteria (AmpR) in crop soil • Bacteria in soils were analyzed every four months using culturing methods. • Faecal and AmpR bacteria increased and persisted in amended soil for two years. • The proportion of AmpR bacteria in amended soils differed with different biosolids. • No correlation between classical faecal markers and AmpR bacteria was found.
Medical Mycology, 1995
The karotype of Candida albicans 1001, a pathogenic isolate, presents two additional chromosome b... more The karotype of Candida albicans 1001, a pathogenic isolate, presents two additional chromosome bands when compared with C. albicans 1006 strain. These two bands were a 2600 kb chromosome located between chromosome group 1-R and chromosome 2 (named chromosome 2*) and a 710 kb small chromosome, called snc due to its similarity in size to the supernumerary chromosome in strain WO-1. A comparison of karyotypes of strains 1001, 1006 and WO-1 has enabled us to conclude that chromosomes 2 and 7 are involved in such a reorganization giving rise to the new chromosome bands of strain 1001. We describe a tentative physical map of C. albicans 1001 based on the previously outlined map of C. albicans strain 1006.
Soil Biology and Biochemistry, 2012
ABSTRACT
Proceedings of the National Academy of Sciences, 2002
Protein degradation by the ubiquitin (Ub) system controls the concentrations of many regulatory p... more Protein degradation by the ubiquitin (Ub) system controls the concentrations of many regulatory proteins. The degradation signals (degrons) of these proteins are recognized by the system's Ub ligases (complexes of E2 and E3 enzymes). Two substrate-binding sites of UBR1, the E3 of the N-end rule pathway in the yeast Saccharomyces cerevisiae , recognize basic (type 1) and bulky hydrophobic (type 2) N-terminal residues of proteins or short peptides. A third substrate-binding site of UBR1 targets CUP9, a transcriptional repressor of the peptide transporter PTR2, through an internal (non-N-terminal) degron of CUP9. Previous work demonstrated that dipeptides with destabilizing N-terminal residues allosterically activate UBR1, leading to accelerated in vivo degradation of CUP9 and the induction of PTR2 expression. Through this positive feedback, S. cerevisiae can sense the presence of extracellular peptides and react by accelerating their uptake. Here, we show that dipeptides with dest...
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Papers by Federico Navarro-Garcia