Microalgae-based biodiesel synthesis is currently not commercially viable due to the high costs o... more Microalgae-based biodiesel synthesis is currently not commercially viable due to the high costs of culture realizations and low lipid yields. The main objective of the current study was to determine the possibility of growing Nannochloropsis oceanica on Saccharomyces cerevisiae yeast wastewater for biodiesel generation at an economical rate. N. oceanica was grown in Guillard F/2 synthetic medium and three dilutions of yeast wastewater (1, 1.25, and 1.5%). Biodiesel properties, in addition to carbohydrate, protein, lipid, dry weight, biomass, lipid productivity, amino acids, and fatty acid methyl ester (FAMEs) content, were analyzed and the quality of the produced biodiesel is assessed. The data revealed the response of N. oceanica to nitrogen-deficiency in the three dilutions of yeast wastewater. N. oceanica in Y2 (1.25%) yeast wastewater dilution exhibited the highest total carbohydrate and lipid percentages (21.19% and 41.97%, respectively), and the highest lipid productivity (52....
Background Biosynthesis of nanoparticles has fueled using materials to produce different metallic... more Background Biosynthesis of nanoparticles has fueled using materials to produce different metallic nanoforms for aquaculture applications. This study investigates the capability of marine Aspergillus flavus amylase (Amy) to synthesize silver nanoparticles (AgNPs) and studied its impact on Dicentrarchus labrax (sea bass) larvae growth performance. Characterization of Amy-AgNPs was carried out by FTIR, UV–Vis’s spectroscopy and SEM. The effect of Amy-AgNPs for antimicrobial and antioxidant were studied.Results The colloidal brown color of Amy-AgNPs with FTIR analysis indicated that amylases were responsible for the capping of the nanoparticles and made it more stable. Spherical Amy-AgNPs had size of 22.88 to 26.35 nm. Significantly, Amy-AgNPs inhibited the growth of fish tested microbes by maximum zone of inhibition 13-20 mm and maximum MICs ranged from 1.6 to 6.3 µg/ml. Also, it acted as scavenged DPPH by 31.46 to 94.0 %. Sea bass larval morphometric measurements including: mean total...
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2018
This paper describes a preliminary analysis of the possibility to use different algae species for... more This paper describes a preliminary analysis of the possibility to use different algae species for biodiesel production. A lab scale cultivation of five Chlorella spp. was conducted to evaluate their potential for biodiesel production, with respect to their growth and fatty acids characterization, as an initial step to transferring them into the outdoor open ponds. The results of algal dry wt (mg/l), arranged in descending order, were Chlorella salina, 200 ± 0.02; Chlorella vulgaris , 192.28 ± 0.00; Chlorella stigmatophora , 162 ± 13.06; Chlorella capsulata , 101.08 ± 7.54; Chlorella marina , 86 ± 6.99, while the growth rates (mg/d) were Chlorella marina , 2 ± 0.17; Chlorella vulgaris , 1.78 ± 0.14; Chlorella stigmatophora , 1.52 ± 0.11; Chlorella capsulata , 1.51 ± 0.13; Chlorella salina , 1.16 ± 0.09. The highest lipid content (dry wt based) was recorded for Chlorella capsulata (446 ± 0.33 mg/g), while Chlorella vulgaris showed the lowest content (255 ± 2.5 mg/g). The amounts of th...
Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease ... more Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen. Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography. Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, an...
Microalgae-based biodiesel synthesis is currently not commercially viable due to the high costs o... more Microalgae-based biodiesel synthesis is currently not commercially viable due to the high costs of culture realizations and low lipid yields. The main objective of the current study was to determine the possibility of growing Nannochloropsis oceanica on Saccharomyces cerevisiae yeast wastewater for biodiesel generation at an economical rate. N. oceanica was grown in Guillard F/2 synthetic medium and three dilutions of yeast wastewater (1, 1.25, and 1.5%). Biodiesel properties, in addition to carbohydrate, protein, lipid, dry weight, biomass, lipid productivity, amino acids, and fatty acid methyl ester (FAMEs) content, were analyzed and the quality of the produced biodiesel is assessed. The data revealed the response of N. oceanica to nitrogen-deficiency in the three dilutions of yeast wastewater. N. oceanica in Y2 (1.25%) yeast wastewater dilution exhibited the highest total carbohydrate and lipid percentages (21.19% and 41.97%, respectively), and the highest lipid productivity (52....
Background Biosynthesis of nanoparticles has fueled using materials to produce different metallic... more Background Biosynthesis of nanoparticles has fueled using materials to produce different metallic nanoforms for aquaculture applications. This study investigates the capability of marine Aspergillus flavus amylase (Amy) to synthesize silver nanoparticles (AgNPs) and studied its impact on Dicentrarchus labrax (sea bass) larvae growth performance. Characterization of Amy-AgNPs was carried out by FTIR, UV–Vis’s spectroscopy and SEM. The effect of Amy-AgNPs for antimicrobial and antioxidant were studied.Results The colloidal brown color of Amy-AgNPs with FTIR analysis indicated that amylases were responsible for the capping of the nanoparticles and made it more stable. Spherical Amy-AgNPs had size of 22.88 to 26.35 nm. Significantly, Amy-AgNPs inhibited the growth of fish tested microbes by maximum zone of inhibition 13-20 mm and maximum MICs ranged from 1.6 to 6.3 µg/ml. Also, it acted as scavenged DPPH by 31.46 to 94.0 %. Sea bass larval morphometric measurements including: mean total...
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2018
This paper describes a preliminary analysis of the possibility to use different algae species for... more This paper describes a preliminary analysis of the possibility to use different algae species for biodiesel production. A lab scale cultivation of five Chlorella spp. was conducted to evaluate their potential for biodiesel production, with respect to their growth and fatty acids characterization, as an initial step to transferring them into the outdoor open ponds. The results of algal dry wt (mg/l), arranged in descending order, were Chlorella salina, 200 ± 0.02; Chlorella vulgaris , 192.28 ± 0.00; Chlorella stigmatophora , 162 ± 13.06; Chlorella capsulata , 101.08 ± 7.54; Chlorella marina , 86 ± 6.99, while the growth rates (mg/d) were Chlorella marina , 2 ± 0.17; Chlorella vulgaris , 1.78 ± 0.14; Chlorella stigmatophora , 1.52 ± 0.11; Chlorella capsulata , 1.51 ± 0.13; Chlorella salina , 1.16 ± 0.09. The highest lipid content (dry wt based) was recorded for Chlorella capsulata (446 ± 0.33 mg/g), while Chlorella vulgaris showed the lowest content (255 ± 2.5 mg/g). The amounts of th...
Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease ... more Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen. Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography. Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, an...
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