Vine decline is limiting muskmelon production in many growing areas. Monosporascus cannonballus P... more Vine decline is limiting muskmelon production in many growing areas. Monosporascus cannonballus Pollack and Uecker and Acremonium cucurbitacearum Alfaro-García, W. Gams, and J. García-Jiménez are the main causal agents of this disease in Spain. The wild accession Pat81 (Cucumis melo subsp. agrestis Jeffrey) has shown to be highly resistant in naturally infested fields and after artificial inoculations. In three greenhouse experiments conducted over two seasons, the root structure of Pat81 was examined and compared to the highly susceptible commercial cultivar Amarillo Canario (AC). Pat81 produced a more vigorous, branched, and longer root system, conferring to this accession a higher capacity for the uptake of water and nutrients, even after inoculation using naturally infested soil. To determine the plasticity of the root systems, the effect of five different soil substrates on root growth was assayed. The root morphology was highly influenced by the soil substrate. Differences ...
Journal of the American Society for Horticultural Science, 2016
Wild relatives represent a source of variation for many traits of interest for eggplant (Solanum ... more Wild relatives represent a source of variation for many traits of interest for eggplant (Solanum melongena) breeding, as well as for broadening the genetic base of this crop. However, interspecific hybridization with wild relatives has been barely used in eggplant breeding programs. As initiation of an introgression breeding program we performed 1424 interspecific hybridizations between six accessions of eggplant from the Occidental and Oriental groups and 19 accessions of 12 wild species from the primary (Solanum incanum and Solanum insanum), secondary (Solanum anguivi, Solanum dasyphyllum, Solanum lichtensteinii, Solanum linnaeanum, Solanum pyracanthos, Solanum tomentosum, and Solanum violaceum), and tertiary (Solanum elaeagnifolium, Solanum sisymbriifolium, and Solanum torvum) genepools. Fruit set, hybrid seed, and seed germination were obtained between Solanum melongena and all wild species of the primary and secondary genepools. The highest fruit set percentage and quantity of ...
The ability to access alleles from unadapted germplasm collections is a long-standing problem for... more The ability to access alleles from unadapted germplasm collections is a long-standing problem for geneticist and breeders. Here we developed, characterized, and demonstrated the utility of a wild barley advanced backcross nested association mapping (AB-NAM) population. We developed this population by backcrossing 25 wild barley accessions to the 6-rowed malting barley cultivar Rasmusson. The 25 wild barley parents were selected from the 318 accession Wild Barley Diversity Collection (WBDC) to maximize allelic diversity. The resulting 796 BC2F4:6 lines were genotyped with 384-SNP markers, and an additional 4,022 SNPs and 263,531 sequence variants were imputed onto the population using 9K iSelect SNP genotypes and exome capture sequence of the parents, respectively. On average, 96% of each wild parent was introgressed into the Rasmusson background, and the population exhibited low population structure. While LD decay (r(2) = 0.2) was lowest in the WBDC (0.36 cM), the AB-NAM (9.2 cM) e...
Eggplant (Solanum melongena) varieties with increased levels of phenolics in the fruit present en... more Eggplant (Solanum melongena) varieties with increased levels of phenolics in the fruit present enhanced functional quality, but may display greater fruit flesh browning. We evaluated 18 eggplant accessions for fruit total phenolics content, chlorogenic acid content, DPPH scavenging activity, polyphenol oxidase (PPO) activity, liquid extract browning, and fruit flesh browning. For all the traits we found a high diversity, with differences among accessions of up to 3.36-fold for fruit flesh browning. Variation in total content in phenolics and in chlorogenic acid content accounted only for 18.9% and 6.0% in the variation in fruit flesh browning, and PPO activity was not significantly correlated with fruit flesh browning. Liquid extract browning was highly correlated with chlorogenic acid content (r=0.852). Principal components analysis (PCA) identified four groups of accessions with different profiles for the traits studied. Results suggest that it is possible to develop new eggplant varieties with improved functional and apparent quality.
Enhancing conservation and use of local vegetable landraces: the Almagro eggplant (Solanum melong... more Enhancing conservation and use of local vegetable landraces: the Almagro eggplant (Solanum melongena L.
Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato... more Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato. Exploitation of resistance to viruses Julián, O.
ABSTRACT In this paper, we have studied the rapid synthesis and antimicrobial activity of silver ... more ABSTRACT In this paper, we have studied the rapid synthesis and antimicrobial activity of silver nanoparticles synthesized using extract of Eichornia crassipes (Water Hyacinth) which is considered as one of the most notorious aquatic weeds present all over the world. The synthesized nanoparticles exhibited absorption maximum at 420 nm which is a characteristic feature of Surface Plasmon Resonance (SPR) peak for silver nanoparticles. The silver nanoparticles were characterized with Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM) and found the size ranges from 10 to 50 nm. Further Fourier Transformation Infrared Spectrum (FTIR) revealed that the phenolic groups present in the plant extract were responsible for the reduction of silver nitrate into silver nanoparticles and stabilization of formed silver nanoparticles. Finally antibacterial activities of the silver nanoparticles were checked against Bacillus subtilis, Pseudomonas fluorescens and Klebsiela pneumonia and significant zones of inhibition were found.
... eggplant relatives (Solanum vespertilio Aiton and Solanum lidii Sunding) endemic to the Canar... more ... eggplant relatives (Solanum vespertilio Aiton and Solanum lidii Sunding) endemic to the Canary Islands Jaime Prohens Æ Gregory J. Anderson Æ F. Javier Herraiz Æ Gabriel Bernardello Æ Arnoldo Santos-Guerra Æ Daniel Crawford Æ Fernando Nuez ...
and gboma (S. macrocarpon) eggplants are important vegetables in Sub-Saharan Africa. Few studies ... more and gboma (S. macrocarpon) eggplants are important vegetables in Sub-Saharan Africa. Few studies have been made on these crops regarding the diversity of phenolic content and their biological activity. We have studied the reducing activity, the chlorogenic acid and other phenolic acid contents in a collection of 56 accessions of scarlet eggplant, including the four cultivated groups (Aculeatum, Gilo, Kumba, Shum) and the weedy intermediate S. aethiopicum-S. anguivi types, as well as in eight accessions of gboma eggplant, including the cultivated S. macrocarpon and its wild ancestor, S. dasyphyllum. A sample of the accessions evaluated in this collection has been tested for inhibition of nitric oxide (NO) using macrophage cell cultures. The results show that there is a great diversity in both crops for reducing activity, chlorogenic acid content and chlorogenic acid peak area ( % of total phenolic acids). Heritability (H2) for these traits was intermediate to high in both crops. In a...
Availability of standardized morphological and molecular characterization data is essential for t... more Availability of standardized morphological and molecular characterization data is essential for the efficient development of breeding programmes in emerging crops. Pepino (Solanum muricatum) is an increasingly important vegetatively propagated vegetable crop for which concurrent data on morphological descriptors and molecular markers are not available. We evaluated 58 morphological traits, using a collection of 14 accessions of pepinos (including local Andean varieties and modern cultivars) and 8 of wild relatives, using the IPGRI and COMAV descriptors lists coupled with 20 EST-SSRs from tomato. High morphological diversity was found in both cultivated and wild accessions; all morphological traits except three were variable. Cultivated pepino and wild relatives were significantly different for 26 traits. Also, local varieties and modern cultivars of pepino were different from each other for 13 morphological traits and were clearly separated in a principal components analysis. Fourte...
Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We ... more Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore in vitro culture. Up to 476,903 microspores and pollen grains per male flower, with in vivo microspore viability rates from 53.71 to 70.88% were found. A high uniformity in the developmental stage of microspores and pollen grains contained in anthers was observed, and this allowed the identification of bud length intervals containing mostly vacuolate microspores and young bi-cellular pollen grains. The starch presence in C. sativa microspores and pollen grains follows a similar pattern to that observed i...
Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphi... more Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants
Vine decline is limiting muskmelon production in many growing areas. Monosporascus cannonballus P... more Vine decline is limiting muskmelon production in many growing areas. Monosporascus cannonballus Pollack and Uecker and Acremonium cucurbitacearum Alfaro-García, W. Gams, and J. García-Jiménez are the main causal agents of this disease in Spain. The wild accession Pat81 (Cucumis melo subsp. agrestis Jeffrey) has shown to be highly resistant in naturally infested fields and after artificial inoculations. In three greenhouse experiments conducted over two seasons, the root structure of Pat81 was examined and compared to the highly susceptible commercial cultivar Amarillo Canario (AC). Pat81 produced a more vigorous, branched, and longer root system, conferring to this accession a higher capacity for the uptake of water and nutrients, even after inoculation using naturally infested soil. To determine the plasticity of the root systems, the effect of five different soil substrates on root growth was assayed. The root morphology was highly influenced by the soil substrate. Differences ...
Journal of the American Society for Horticultural Science, 2016
Wild relatives represent a source of variation for many traits of interest for eggplant (Solanum ... more Wild relatives represent a source of variation for many traits of interest for eggplant (Solanum melongena) breeding, as well as for broadening the genetic base of this crop. However, interspecific hybridization with wild relatives has been barely used in eggplant breeding programs. As initiation of an introgression breeding program we performed 1424 interspecific hybridizations between six accessions of eggplant from the Occidental and Oriental groups and 19 accessions of 12 wild species from the primary (Solanum incanum and Solanum insanum), secondary (Solanum anguivi, Solanum dasyphyllum, Solanum lichtensteinii, Solanum linnaeanum, Solanum pyracanthos, Solanum tomentosum, and Solanum violaceum), and tertiary (Solanum elaeagnifolium, Solanum sisymbriifolium, and Solanum torvum) genepools. Fruit set, hybrid seed, and seed germination were obtained between Solanum melongena and all wild species of the primary and secondary genepools. The highest fruit set percentage and quantity of ...
The ability to access alleles from unadapted germplasm collections is a long-standing problem for... more The ability to access alleles from unadapted germplasm collections is a long-standing problem for geneticist and breeders. Here we developed, characterized, and demonstrated the utility of a wild barley advanced backcross nested association mapping (AB-NAM) population. We developed this population by backcrossing 25 wild barley accessions to the 6-rowed malting barley cultivar Rasmusson. The 25 wild barley parents were selected from the 318 accession Wild Barley Diversity Collection (WBDC) to maximize allelic diversity. The resulting 796 BC2F4:6 lines were genotyped with 384-SNP markers, and an additional 4,022 SNPs and 263,531 sequence variants were imputed onto the population using 9K iSelect SNP genotypes and exome capture sequence of the parents, respectively. On average, 96% of each wild parent was introgressed into the Rasmusson background, and the population exhibited low population structure. While LD decay (r(2) = 0.2) was lowest in the WBDC (0.36 cM), the AB-NAM (9.2 cM) e...
Eggplant (Solanum melongena) varieties with increased levels of phenolics in the fruit present en... more Eggplant (Solanum melongena) varieties with increased levels of phenolics in the fruit present enhanced functional quality, but may display greater fruit flesh browning. We evaluated 18 eggplant accessions for fruit total phenolics content, chlorogenic acid content, DPPH scavenging activity, polyphenol oxidase (PPO) activity, liquid extract browning, and fruit flesh browning. For all the traits we found a high diversity, with differences among accessions of up to 3.36-fold for fruit flesh browning. Variation in total content in phenolics and in chlorogenic acid content accounted only for 18.9% and 6.0% in the variation in fruit flesh browning, and PPO activity was not significantly correlated with fruit flesh browning. Liquid extract browning was highly correlated with chlorogenic acid content (r=0.852). Principal components analysis (PCA) identified four groups of accessions with different profiles for the traits studied. Results suggest that it is possible to develop new eggplant varieties with improved functional and apparent quality.
Enhancing conservation and use of local vegetable landraces: the Almagro eggplant (Solanum melong... more Enhancing conservation and use of local vegetable landraces: the Almagro eggplant (Solanum melongena L.
Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato... more Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato. Exploitation of resistance to viruses Julián, O.
ABSTRACT In this paper, we have studied the rapid synthesis and antimicrobial activity of silver ... more ABSTRACT In this paper, we have studied the rapid synthesis and antimicrobial activity of silver nanoparticles synthesized using extract of Eichornia crassipes (Water Hyacinth) which is considered as one of the most notorious aquatic weeds present all over the world. The synthesized nanoparticles exhibited absorption maximum at 420 nm which is a characteristic feature of Surface Plasmon Resonance (SPR) peak for silver nanoparticles. The silver nanoparticles were characterized with Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM) and found the size ranges from 10 to 50 nm. Further Fourier Transformation Infrared Spectrum (FTIR) revealed that the phenolic groups present in the plant extract were responsible for the reduction of silver nitrate into silver nanoparticles and stabilization of formed silver nanoparticles. Finally antibacterial activities of the silver nanoparticles were checked against Bacillus subtilis, Pseudomonas fluorescens and Klebsiela pneumonia and significant zones of inhibition were found.
... eggplant relatives (Solanum vespertilio Aiton and Solanum lidii Sunding) endemic to the Canar... more ... eggplant relatives (Solanum vespertilio Aiton and Solanum lidii Sunding) endemic to the Canary Islands Jaime Prohens Æ Gregory J. Anderson Æ F. Javier Herraiz Æ Gabriel Bernardello Æ Arnoldo Santos-Guerra Æ Daniel Crawford Æ Fernando Nuez ...
and gboma (S. macrocarpon) eggplants are important vegetables in Sub-Saharan Africa. Few studies ... more and gboma (S. macrocarpon) eggplants are important vegetables in Sub-Saharan Africa. Few studies have been made on these crops regarding the diversity of phenolic content and their biological activity. We have studied the reducing activity, the chlorogenic acid and other phenolic acid contents in a collection of 56 accessions of scarlet eggplant, including the four cultivated groups (Aculeatum, Gilo, Kumba, Shum) and the weedy intermediate S. aethiopicum-S. anguivi types, as well as in eight accessions of gboma eggplant, including the cultivated S. macrocarpon and its wild ancestor, S. dasyphyllum. A sample of the accessions evaluated in this collection has been tested for inhibition of nitric oxide (NO) using macrophage cell cultures. The results show that there is a great diversity in both crops for reducing activity, chlorogenic acid content and chlorogenic acid peak area ( % of total phenolic acids). Heritability (H2) for these traits was intermediate to high in both crops. In a...
Availability of standardized morphological and molecular characterization data is essential for t... more Availability of standardized morphological and molecular characterization data is essential for the efficient development of breeding programmes in emerging crops. Pepino (Solanum muricatum) is an increasingly important vegetatively propagated vegetable crop for which concurrent data on morphological descriptors and molecular markers are not available. We evaluated 58 morphological traits, using a collection of 14 accessions of pepinos (including local Andean varieties and modern cultivars) and 8 of wild relatives, using the IPGRI and COMAV descriptors lists coupled with 20 EST-SSRs from tomato. High morphological diversity was found in both cultivated and wild accessions; all morphological traits except three were variable. Cultivated pepino and wild relatives were significantly different for 26 traits. Also, local varieties and modern cultivars of pepino were different from each other for 13 morphological traits and were clearly separated in a principal components analysis. Fourte...
Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We ... more Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore in vitro culture. Up to 476,903 microspores and pollen grains per male flower, with in vivo microspore viability rates from 53.71 to 70.88% were found. A high uniformity in the developmental stage of microspores and pollen grains contained in anthers was observed, and this allowed the identification of bud length intervals containing mostly vacuolate microspores and young bi-cellular pollen grains. The starch presence in C. sativa microspores and pollen grains follows a similar pattern to that observed i...
Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphi... more Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants
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Papers by Javier Herraiz