Papers by INTERNATIONAL JOURNAL
IJUPBS Journal, 2013
Ursolic acid (UA) is a vital bioactive component of Eucalyptus × hybrida possessing anticancer, a... more Ursolic acid (UA) is a vital bioactive component of Eucalyptus × hybrida possessing anticancer, antimutagenic, anti-inflammatory and antiprotozoal activities. The present study aims at finding antioxidant and antimicrobial potential of Eucalyptus × hybrida extracts, setting up solvent scheme for high yield extraction of ursolic acid and standardization of chromatographic separation of ursolic acid from Eucalyptus x hybrida extracts.
Biological methods of synthesis have paved way for " greener synthesis " of nanoparticles and the... more Biological methods of synthesis have paved way for " greener synthesis " of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. This has motivated an upsurge in research on the synthesis routes that allow better control of shape and size for various nanotechnological applications. In this work, we have explored an inventive contribution for synthesis of silver nanoparticles using catharanthus roseus (Sadabahar), Musa paradisiacal (Plantain flower) and Polygonum odoratum (Coriandium) leaf extract. Synthesized nanoparticles were characterized by various methods such as UV-Vis spectroscopy, SEM and XRD. In addition, antibacterial activity of the synthesized silver nanoparticles was also determined. This new method is rapid time scales for biosynthesis of metallic nanoparticles using environmentally benign natural resources as an alternative to chemical synthesis protocols as reductant for synthesizing silver nanoparticles. 250 | P a g e
Amongst the plethora of avenues explored for rapid drug releasing products, oral strip technology... more Amongst the plethora of avenues explored for rapid drug releasing products, oral strip technology (OST) is gaining much attention and acceptance. They has been accepted as a new drug delivery system to enhance safety and efficacy of drug molecule by formulating it into an oral dosage. These films are solid dosage form very similar to postage stamp in shape, size and thickness which disintegrate and dissolve in 1min when placed on tongue without drinking water and chewing. These are flexible therefore no need of special packaging is required. They provide better drug utilization in bypassing the first pass metabolism and making the medication more bioavailable [1,2]. This also helps in improving the onset of action, lower the dosing, enhance stability, provides site specific and local action and eliminate patient fear of choking. Their formulation involves the application of both aesthetic and performance characteristics such as plasticized hydrocolloids, active pharmaceutical ingredient, taste masking agent being laminated by solvent casting method, semi solid casting method, hot melt extrusion, solid dispersion extrusion and rolling method [5]. Their evaluation parameters include identification of thickness, folding endurance, percentage elongation, swelling index, tensile strength, stability studies, disintegration and dissolution time. 236 | P a g e
Ultra performance liquid chromatography (UPLC) is a relatively new technique giving new possibili... more Ultra performance liquid chromatography (UPLC) is a relatively new technique giving new possibilities in liquid chromatography, especially concerning decreases of time and solvent consumptions. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. Special analytical columns UPLC Acquity UPLC BEH C18 packed with 1.7_m particles are used in connection with this system. UPLC refers to ultra performance liquid chromatography, which improves in three areas : " speed, resolution and sensitivity ". Today's pharmaceutical industries are looking for new ways to cut cost and shorten time for development of drugs while at the same time improving the quality of their products and analytical laboratories are not exception in this trend. Speed allows a greater number of analyses to be performed in a shorter amount of time thereby increasing sample throughput and lab productivity. These are the benefits of Ultra performance liquid chromatography. UPLC can be regarded as a new direction for liquid chromatography. UPLC System Take advantage of technological strides made in particle chemistry performance. System optimization, detector design, and data processing and control. These Achievements have created a step-function improvement in chromatographic performance. This review introduces the theory of UPLC, and summarizes some of the most recent work in the field. 224 | P a g e
Diabetes mellitus is a disorder affecting people since the antiquity, but it was known by differe... more Diabetes mellitus is a disorder affecting people since the antiquity, but it was known by different names in different era, and managed accordingly to a greater extent. Today it has reached to an epidemic proportion due to substantial lifestyle modifications. To validate the efficacy of Unani Formulations several clinical trials have been carried out at different institutions of Unani Medicine; in the present study four clinical trials have been included which were carried out in the post graduate department of Moalejat, A.K. Tibbiya College, AMU, Aligarh. The main objective of this study was to compare the result of these clinical trials to find out potent and effective Unani drugs/ formulations. From the observations and results it is evident that the formulation 4 is the most effective. But is to conclude that, the drug formulation 3 (Tukhme Hayat and Tukhme Hulba) is the best combination, because it has strong hypoglycaemic evidences, these drugs are easily available, cost effective and easy to use. Further it is proposed that any combination with 2-3 drugs from the above formulations may be used to manage diabetes mellitus type 2 as these drugs are the key hypoglycaemic agents indicated in classical Unani texts and have been proven scientifically today
Depression is a common disorder observed in plenty of the population. Chronic mild stress is a we... more Depression is a common disorder observed in plenty of the population. Chronic mild stress is a well-established model, whereas acute mild stress has never been tried. The present study investigates behavioural, physiological and biochemical parameters of depression in rats as an aspect of mild stress induced depression. Sucrose preference test, adrenal gland weight, body weight, locomotor activity and brain serotonin levels were evaluated on the stress induced rats. Findings from the study indicate that sucrose consumption as a result of anhedonia and locomotor activity of stressed rats decreased, brain serotonin levels were elevated and there was no significance difference in body weight of stressed and normal control rats.
The analytical method validation process was established for the fixed dosage combination of S (-... more The analytical method validation process was established for the fixed dosage combination of S (-) Amlodipine (Levoamlodipine) Besilate and Losartan potassium tablets. The forced degradation study was carried out for drug substances and for drug product as per ICH guidelines. The peaks were homogeneous as peak threshold value found greater than peak angle. The developed protocol applied to the analytical method validation for the analytical assay method of S (-) Amlodipine Besilate and Losartan potassium for fixed dosage combination by HPLC. The HPLC column having specifications as Inertsil ODS-3 (25cm x 4.6 mm internal diameter, 5µ particle size) and mobile phase comprising of buffer: acetonitrile (75:25) was used. The method applies for strength formulated [S (-) Amlodipine Besilate 5mg and Losartan potassium 50mg/tablet]. Linearity studies showed correlation coefficient as 0.9998 for both the drugs. The % relative standard deviation for precision study was found below 2.0%. The mean recovery was found to be well within limits 98 to 102%. The % RSD for solution stability study showed closeness between values calculated as label claim for active ingredients. For the robustness study the %RSD of label claim was found below 2.0% and assay values within ±2.0% in case of both drugs. The ruggedness study shows % RSD of assay value below 1.91% for both actives. No interference and adsorption of active ingredients was observed for filter papers and these were found to be compatible with formulation. Thus developed analytical method was found to be specific, linear, precise, accurate, robust and rugged.
Validation must be done using scientific and statistical principles; to establish process capabil... more Validation must be done using scientific and statistical principles; to establish process capability to conform product acceptability.
Present study describes the spectrophotometric method development and subsequent validation of Di... more Present study describes the spectrophotometric method development and subsequent validation of Diltiazem hydrochloride and its formulation with greater precision and accuracy. Spectrophotometric measurements were carried out using JASCO double beam (V-530 model) UV-visible spectrophotometer with a pair of 1 mm matched quartz cells and 0.1 N HCL as hydrochloric buffer. λmax was 237 nm. Linearity was observed in the concentration range 5-15μg/mL. The percentage recovery of Diltiazem hydrochloride was in the range 98.9 -101.7%. The precision was found to be NMT 2.0% RSD (Relative standard deviation). Proposed method was validated as per International Conference on Harmonization (ICH)
The problem of short gastric residence time encountered with an oral CR formulation. One of the t... more The problem of short gastric residence time encountered with an oral CR formulation. One of the technologies to overcome this problem is formation of floating drug delivery systems. Hollow microspheres represent this type of system. Hollow microspheres loaded with drug in their outer polymer shelf. The problem of short gastric residence time with an oral CR formulation can be overcome with these systems. These systems have a bulk density of <1 as a result of which they can float on the gastric contents. Hollow microspheres as gastroretentive dosage forms precisely control the release rate of target drug to a specific site and facilitates an enormous impact on health care. Optimized multi-unit floating microspheres are expected to provide clinicians with a new choice of an economical, safe and more bioavailable formulation in the effective management of diverse diseases. These systems also provide tremendous opportunities in the designing of new controlled and delayed release oral formulations, thus extending the frontier of futuristic pharmaceutical development. Gatsroretentive drug delivery system offers several advantages besides providing better bioavailability to poorly absorbed drugs and a required release profile thus attracting interest of pharmaceutical formulation scientists. The purpose of this review is to focus on the recent advances in the field of formulation, characterization, evaluation and applications of floating microspheres in the area of gastroretentive dosage forms. This review attempts to bring more insight into recent advances in methods of fabrication techniques and applications of hollow microspheres.
A liposome is an artificially-prepared vesicle composed of a lipid bilayer. The liposome can be u... more A liposome is an artificially-prepared vesicle composed of a lipid bilayer. The liposome can be used as a vehicle for administration of nutrients and pharmaceutical. Liposomes are composed of natural phospholipids, and may also contain mixed lipid chains with surfactant properties. The major types of liposomes are the multilamellar vesicle, the small unilamellar vesicle, and the large unilamellar vesicle. Liposomal formulations were significantly explored over the last decade for the Anticancer Therapy, Respiratory drug delivery system, Nucleic acid therapy, ophthalmic drug delivery applications, Vaccine adjuvant, Anti-infective, Brain Targeting therapy. These formulations are mainly composed of phosphatidylcholine and other constituents such as cholesterol and lipid-conjugated hydrophilic polymers. Liposomes are biodegradable and biocompatible in nature. In this review article we summarize information about some of the key advantages of liposome, mechanism of liposomal formation, classification of liposomes, and application of liposome.
Cucumis melo Linn. (Cucurbitaceae) fruits have been used, traditionally, for the treatment of var... more Cucumis melo Linn. (Cucurbitaceae) fruits have been used, traditionally, for the treatment of various disorders of heart as Cardioprotective and antiobesity. The aim of the present study was to investigate the possible anti-hyperlipidemic activity of Cucumis melo fruit peel extract in triton induced hyperlipidemia in rats. Chloroform, Methanolic and aqueous extracts of were administered to the triton induced hyperlipidemic rats for 7 days to study antihyperlipidemic acivity. The acute toxicity value of chloroform, methanol and aqueous extract after oral administration in mice were found to be 5000 mg/kg. The results concluded that CMFP methanolic extract (500 mg/kg) have definite antihyperlipidemic activity in Triton X-100 induced hyperlipidemia model and which is equipotent activity when compared with Atorvastatin treated group.
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Papers by INTERNATIONAL JOURNAL