Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997
Two genomic clones of the villin (VIL) gene were independently hybridized on river buffalo (Bubal... more Two genomic clones of the villin (VIL) gene were independently hybridized on river buffalo (Bubalus bubalis, BBU), sheep (Ovis aries, OAR) and goat (Capra hircus, CHI) chromosomes by using sequential fluorescence in situ hybridization (FISH) and R-banding (RBP- and RBA-banding). Clear hybridization signals revealed that VIL is located in BBU 2q33, OAR 2q33 and CHI 2q33. These chromosomes and chromosome bands are believed to be homologous and the VIL locus is the same as that previously found on cattle chromosome 2q43. VIL localization in these three species allows us tentatively to assign all cattle U17 to BBU and CHI 2q and to extend the physical map to OAR 2q.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997
L. Iannuzzi (corresponding author) and GP Di Meo are at the National Research Council (CNR), IAB-... more L. Iannuzzi (corresponding author) and GP Di Meo are at the National Research Council (CNR), IAB-BAM, Via Argine 1085, 80147 Naples, Italy. Tel: (+39) 815 964 977; Fax: (+39) 815 965 291; Email: Poldo@iabbam.iabbam.na.cnr.it. C. Le Chalony and G. Goubin are at ...
An improved characterization of cattle chromosomes was obtained by means of high-resolution G- an... more An improved characterization of cattle chromosomes was obtained by means of high-resolution G- and R-band comparison. Models of G- and R-banded karyotypes that were arranged according to the Reading system and the previous RBA-banded karyotype were obtained at the 475 band level by using early- and late-BrdU incorporation in synchronized cell cultures. As in human chromosomes, only one common G- and R-banding nomenclature is proposed.
Sister chromatid exchanges (SCEs) in early- and late-replicating X chromosomes of seven female ca... more Sister chromatid exchanges (SCEs) in early- and late-replicating X chromosomes of seven female cattle (Bos taurus L.) and five female river buffalo (Bubalus bubalis L.) were studied in untreated lymphocytes and lymphocytes treated with mitomycin C (MMC). In the experiment, 577 SCEs on X chromosomes of MMC-untreated cells and 825 SCEs on X chromosomes of MMC-treated cells from both species were observed. No significant differences between the number of SCEs in early- and late-replicating X chromosomes were found even when singular species and subjects were considered.
The Fragile Histidine Triad gene (FHIT) is an oncosuppressor implicated in many human cancers, in... more The Fragile Histidine Triad gene (FHIT) is an oncosuppressor implicated in many human cancers, including vesical tumors. FHIT is frequently hit by deletions caused by fragility at FRA3B, the most active of human common fragile sites, where FHIT lays. Vesical tumors affect also cattle, including animals grazing in the wild on bracken fern; compounds released by the fern are known to induce chromosome fragility and may trigger cancer with the interplay of latent Papilloma virus. The bovine FHIT was characterized by assembling a contig of 78 BACs. Sequence tags were designed on human exons and introns and used directly to select bovine BACs, or compared with sequence data in the bovine genome database or in the trace archive of the bovine genome sequencing project, and adapted before use. FHIT is split in ten exons like in man, with exons 5 to 9 coding for a 149 amino acids protein. VISTA global alignments between bovine genomic contigs retrieved from the bovine genome database and the...
The management of disorders of sexual development (DSD) in humans and domestic animals has been t... more The management of disorders of sexual development (DSD) in humans and domestic animals has been the subject of intense interest for decades. The association between abnormal chromosome constitutions and DSDs in domestic animals has been recorded since the beginnings of conventional cytogenetic analysis. Deviated karyotypes consisting of abnormal sex chromosome sets and/or the coexistence of cells with different sex chromosome constitutions in an individual seem to be the main causes of anomalies of sex determination and sex differentiation. In recent years, a growing interest has developed around the environmental insults, such as endocrine-disrupting compounds (EDC) and heat stressors, which affect fertility, early embryonic development and, in some instances, directly the sex ratio and/or the development of 1 specific sex versus the other. A variety of chemical compounds present in the environment at low doses has been shown to have major effects on the reproductive functions in human and domestic animals following prolonged exposure. In this review, we present an overview of congenital/chromosomal factors that are responsible for the DSDs and link them and the lack of proper embryonic development to environmental factors that are becoming a major global concern.
High rates of structural chromosome aberrations were associated with increased yields of sister c... more High rates of structural chromosome aberrations were associated with increased yields of sister chromatid exchanges (SCE) in metaphase chromosomes of a new born female calf affected by a congenital malformation. The frequency of abnormal cells was 25 per cent in the abnormal calf, 8 per cent in its dam and 3 per cent in a group of four healthy cows. Chromatid and chromosome breaks were the most frequent types of chromosome aberration found in the malformed calf; centric fusions, chromosome fragments and deletions were much less common. The mean rate of SCE/cell in the malformed calf was nearly twice that of the control and the difference was statistically significant. Possible factors involved in the occurrence of such a malformation are discussed.
Excess thymidine is capable of synchronizing lymphocyte cultures in vitro by acting with a feed-b... more Excess thymidine is capable of synchronizing lymphocyte cultures in vitro by acting with a feed-back mechanism during the S phase of the cell cycle (Harper 2005 Methods Mol. Biol. 296, 157–166). The possibility to synchronize the embryonic growth can be a good strategy for future epigenetic studies. The present study was undertaken to test whether excess thymidine could also synchronize in vitro development of bovine embryos. Abattoir-derived cumulus–oocyte complexes (COC) of the Agerolese breed of cattle were matured in vitro using standard procedures. After maturation, COCs were transferred in drops of 300 µL of IVF-TALP (25/drop) and covered with mineral oil. Frozen sperm from a bull were selected by centrifugation on a Percoll discontinuous gradient (45 to 80%). The pellet was diluted in IVF medium and added to the COC at the concentration of 1 × 106 sperm mL–1. After 18–20 h of gametes co-incubation, presumptive zygotes were denuded and cultured in SOF medium containing differe...
Go to AGRIS search. Atti della Societa' Italiana delle Scienze Veterinarie (2002). The use o... more Go to AGRIS search. Atti della Societa' Italiana delle Scienze Veterinarie (2002). The use of computer-assisted image analysis in building a karyotype of Equidae. ...
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997
Two genomic clones of the villin (VIL) gene were independently hybridized on river buffalo (Bubal... more Two genomic clones of the villin (VIL) gene were independently hybridized on river buffalo (Bubalus bubalis, BBU), sheep (Ovis aries, OAR) and goat (Capra hircus, CHI) chromosomes by using sequential fluorescence in situ hybridization (FISH) and R-banding (RBP- and RBA-banding). Clear hybridization signals revealed that VIL is located in BBU 2q33, OAR 2q33 and CHI 2q33. These chromosomes and chromosome bands are believed to be homologous and the VIL locus is the same as that previously found on cattle chromosome 2q43. VIL localization in these three species allows us tentatively to assign all cattle U17 to BBU and CHI 2q and to extend the physical map to OAR 2q.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997
L. Iannuzzi (corresponding author) and GP Di Meo are at the National Research Council (CNR), IAB-... more L. Iannuzzi (corresponding author) and GP Di Meo are at the National Research Council (CNR), IAB-BAM, Via Argine 1085, 80147 Naples, Italy. Tel: (+39) 815 964 977; Fax: (+39) 815 965 291; Email: Poldo@iabbam.iabbam.na.cnr.it. C. Le Chalony and G. Goubin are at ...
An improved characterization of cattle chromosomes was obtained by means of high-resolution G- an... more An improved characterization of cattle chromosomes was obtained by means of high-resolution G- and R-band comparison. Models of G- and R-banded karyotypes that were arranged according to the Reading system and the previous RBA-banded karyotype were obtained at the 475 band level by using early- and late-BrdU incorporation in synchronized cell cultures. As in human chromosomes, only one common G- and R-banding nomenclature is proposed.
Sister chromatid exchanges (SCEs) in early- and late-replicating X chromosomes of seven female ca... more Sister chromatid exchanges (SCEs) in early- and late-replicating X chromosomes of seven female cattle (Bos taurus L.) and five female river buffalo (Bubalus bubalis L.) were studied in untreated lymphocytes and lymphocytes treated with mitomycin C (MMC). In the experiment, 577 SCEs on X chromosomes of MMC-untreated cells and 825 SCEs on X chromosomes of MMC-treated cells from both species were observed. No significant differences between the number of SCEs in early- and late-replicating X chromosomes were found even when singular species and subjects were considered.
The Fragile Histidine Triad gene (FHIT) is an oncosuppressor implicated in many human cancers, in... more The Fragile Histidine Triad gene (FHIT) is an oncosuppressor implicated in many human cancers, including vesical tumors. FHIT is frequently hit by deletions caused by fragility at FRA3B, the most active of human common fragile sites, where FHIT lays. Vesical tumors affect also cattle, including animals grazing in the wild on bracken fern; compounds released by the fern are known to induce chromosome fragility and may trigger cancer with the interplay of latent Papilloma virus. The bovine FHIT was characterized by assembling a contig of 78 BACs. Sequence tags were designed on human exons and introns and used directly to select bovine BACs, or compared with sequence data in the bovine genome database or in the trace archive of the bovine genome sequencing project, and adapted before use. FHIT is split in ten exons like in man, with exons 5 to 9 coding for a 149 amino acids protein. VISTA global alignments between bovine genomic contigs retrieved from the bovine genome database and the...
The management of disorders of sexual development (DSD) in humans and domestic animals has been t... more The management of disorders of sexual development (DSD) in humans and domestic animals has been the subject of intense interest for decades. The association between abnormal chromosome constitutions and DSDs in domestic animals has been recorded since the beginnings of conventional cytogenetic analysis. Deviated karyotypes consisting of abnormal sex chromosome sets and/or the coexistence of cells with different sex chromosome constitutions in an individual seem to be the main causes of anomalies of sex determination and sex differentiation. In recent years, a growing interest has developed around the environmental insults, such as endocrine-disrupting compounds (EDC) and heat stressors, which affect fertility, early embryonic development and, in some instances, directly the sex ratio and/or the development of 1 specific sex versus the other. A variety of chemical compounds present in the environment at low doses has been shown to have major effects on the reproductive functions in human and domestic animals following prolonged exposure. In this review, we present an overview of congenital/chromosomal factors that are responsible for the DSDs and link them and the lack of proper embryonic development to environmental factors that are becoming a major global concern.
High rates of structural chromosome aberrations were associated with increased yields of sister c... more High rates of structural chromosome aberrations were associated with increased yields of sister chromatid exchanges (SCE) in metaphase chromosomes of a new born female calf affected by a congenital malformation. The frequency of abnormal cells was 25 per cent in the abnormal calf, 8 per cent in its dam and 3 per cent in a group of four healthy cows. Chromatid and chromosome breaks were the most frequent types of chromosome aberration found in the malformed calf; centric fusions, chromosome fragments and deletions were much less common. The mean rate of SCE/cell in the malformed calf was nearly twice that of the control and the difference was statistically significant. Possible factors involved in the occurrence of such a malformation are discussed.
Excess thymidine is capable of synchronizing lymphocyte cultures in vitro by acting with a feed-b... more Excess thymidine is capable of synchronizing lymphocyte cultures in vitro by acting with a feed-back mechanism during the S phase of the cell cycle (Harper 2005 Methods Mol. Biol. 296, 157–166). The possibility to synchronize the embryonic growth can be a good strategy for future epigenetic studies. The present study was undertaken to test whether excess thymidine could also synchronize in vitro development of bovine embryos. Abattoir-derived cumulus–oocyte complexes (COC) of the Agerolese breed of cattle were matured in vitro using standard procedures. After maturation, COCs were transferred in drops of 300 µL of IVF-TALP (25/drop) and covered with mineral oil. Frozen sperm from a bull were selected by centrifugation on a Percoll discontinuous gradient (45 to 80%). The pellet was diluted in IVF medium and added to the COC at the concentration of 1 × 106 sperm mL–1. After 18–20 h of gametes co-incubation, presumptive zygotes were denuded and cultured in SOF medium containing differe...
Go to AGRIS search. Atti della Societa' Italiana delle Scienze Veterinarie (2002). The use o... more Go to AGRIS search. Atti della Societa' Italiana delle Scienze Veterinarie (2002). The use of computer-assisted image analysis in building a karyotype of Equidae. ...
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