A species of Sarcocystis is reported from a naturally infected African grey parrot, Psittacus eri... more A species of Sarcocystis is reported from a naturally infected African grey parrot, Psittacus erithacus, from Costa Rica. Only mature sarcocysts, measuring up to 2 mm in length and up to 750 μm in width, were observed. The sarcocyst wall was smooth. The villar protrusions on the sarcocyst wall were up to 5 μm long and up to 1.1 μm
The silvery gibbon ( Hylobates moloch ), endemic to the island of Java, relies on closed-canopy, ... more The silvery gibbon ( Hylobates moloch ), endemic to the island of Java, relies on closed-canopy, low- land evergreen forest for its survival. Because Java has lost over 91% of its original forest, silvery gibbons cur- rently occupy small, isolated forest fragments and are threatened with extinction. To contribute to a compre- hensive conservation strategy for this species, we analyzed
This work presents the study by external PIXE of two giant submandibular obtained from different ... more This work presents the study by external PIXE of two giant submandibular obtained from different individuals. The backscattered protons were detected to perform Rutherford Backscattering Spectrometry (RBS) simultaneously to PIXE. These calculi are rare and presented a layered structure; an elemental profile was obtained: P, Ca, O are commonly detected; Cl, Fe, Cu, Zn, Br and Sr are detected in
A relevant problem related to competitive electricity markets operating under the concept of pool... more A relevant problem related to competitive electricity markets operating under the concept of pool model is the allocation of the congestion cost among the consumers. Typically, this cost is allocated to consumers using simple approaches such as pro rata criteria that fail to comply with economic efficiency requirements. This paper proposes an approach for congestion cost allocation based on the
Hepatitis C virus Core is an intriguing protein with important roles in life cycle of this pathog... more Hepatitis C virus Core is an intriguing protein with important roles in life cycle of this pathogen. In the present work, recombinant vaccinia viruses expressing a genotype 1b HCV Core protein, individually (vvCore) or as a polyprotein Core-E1-E2 (vvRE), were generated and characterized. In general, viral titer of recombinant vaccinia viruses ex- pressing the Core protein in BSC40 infected cells
This article describes a case of massive mortality among horses which was probably due to intoxic... more This article describes a case of massive mortality among horses which was probably due to intoxication by pyrrolizidine alkaloids from Heliotropium indicum. Over 4 years more than 75% of a population of about 110 horses on a farm in Costa Rica died after showing nervous neurological symptoms. Two clinical manifestations were encountered, an acute and a chronic one, both with
SUMMARY Hepatitis C virus (HCV) infection represents a major problem of public health worldwide. ... more SUMMARY Hepatitis C virus (HCV) infection represents a major problem of public health worldwide. In the present study, an attempt was made to review data on morphological lesions in the liver in the course of chronic type C hepatitis. Cell lesions detected by light microscopy show characteristic, although not pathognomonic histological changes. These consist of lymphoid aggregates in portal tracts,
The taxonomic position of the endemic New Zealand bat genus Mystacina has vexed systematists ever... more The taxonomic position of the endemic New Zealand bat genus Mystacina has vexed systematists ever since its erection in 1843. Over the years the genus has been linked with many microchiropteran families and superfamilies. Most recent classifications place it in the Vespertilionoidea, although some immunological evidence links it with the Noctilionoidea (=Phyllostomoidea). We have sequenced 402 bp of the mitochondrial cytochrome b gene for M. tuberculata (Gray in Dieffenbach, 1843), and using both our own and published DNA sequences for taxa in both superfamilies, we applied different tree reconstruction methods to find the appropriate phylogeny and different methods of estimating confidence in the parts of the tree. All methods strongly support the classification of Mystacina in the Noctilionoidea. Spectral analysis suggests that parsimony analysis may be misleading for Mystacina's precise placement within the Noctilionoidea because of its long terminal branch. Analyses not susceptible to long-branch attraction suggest that the Mystacinidae is a sister family to the Phyllostomidae. Dating the divergence times between the different taxa suggests that the extant chiropteran families radiated around and shortly after the Cretaceous-Tertiary boundary. We discuss the biogeographical implications of classifying Mystacina within the Noctilionoidea and contrast our result with those classifications placing Mystacina in the Vespertilionoidea, concluding that evidence for the latter is weak.
A species of Sarcocystis is reported from a naturally infected African grey parrot, Psittacus eri... more A species of Sarcocystis is reported from a naturally infected African grey parrot, Psittacus erithacus, from Costa Rica. Only mature sarcocysts, measuring up to 2 mm in length and up to 750 μm in width, were observed. The sarcocyst wall was smooth. The villar protrusions on the sarcocyst wall were up to 5 μm long and up to 1.1 μm
The silvery gibbon ( Hylobates moloch ), endemic to the island of Java, relies on closed-canopy, ... more The silvery gibbon ( Hylobates moloch ), endemic to the island of Java, relies on closed-canopy, low- land evergreen forest for its survival. Because Java has lost over 91% of its original forest, silvery gibbons cur- rently occupy small, isolated forest fragments and are threatened with extinction. To contribute to a compre- hensive conservation strategy for this species, we analyzed
This work presents the study by external PIXE of two giant submandibular obtained from different ... more This work presents the study by external PIXE of two giant submandibular obtained from different individuals. The backscattered protons were detected to perform Rutherford Backscattering Spectrometry (RBS) simultaneously to PIXE. These calculi are rare and presented a layered structure; an elemental profile was obtained: P, Ca, O are commonly detected; Cl, Fe, Cu, Zn, Br and Sr are detected in
A relevant problem related to competitive electricity markets operating under the concept of pool... more A relevant problem related to competitive electricity markets operating under the concept of pool model is the allocation of the congestion cost among the consumers. Typically, this cost is allocated to consumers using simple approaches such as pro rata criteria that fail to comply with economic efficiency requirements. This paper proposes an approach for congestion cost allocation based on the
Hepatitis C virus Core is an intriguing protein with important roles in life cycle of this pathog... more Hepatitis C virus Core is an intriguing protein with important roles in life cycle of this pathogen. In the present work, recombinant vaccinia viruses expressing a genotype 1b HCV Core protein, individually (vvCore) or as a polyprotein Core-E1-E2 (vvRE), were generated and characterized. In general, viral titer of recombinant vaccinia viruses ex- pressing the Core protein in BSC40 infected cells
This article describes a case of massive mortality among horses which was probably due to intoxic... more This article describes a case of massive mortality among horses which was probably due to intoxication by pyrrolizidine alkaloids from Heliotropium indicum. Over 4 years more than 75% of a population of about 110 horses on a farm in Costa Rica died after showing nervous neurological symptoms. Two clinical manifestations were encountered, an acute and a chronic one, both with
SUMMARY Hepatitis C virus (HCV) infection represents a major problem of public health worldwide. ... more SUMMARY Hepatitis C virus (HCV) infection represents a major problem of public health worldwide. In the present study, an attempt was made to review data on morphological lesions in the liver in the course of chronic type C hepatitis. Cell lesions detected by light microscopy show characteristic, although not pathognomonic histological changes. These consist of lymphoid aggregates in portal tracts,
The taxonomic position of the endemic New Zealand bat genus Mystacina has vexed systematists ever... more The taxonomic position of the endemic New Zealand bat genus Mystacina has vexed systematists ever since its erection in 1843. Over the years the genus has been linked with many microchiropteran families and superfamilies. Most recent classifications place it in the Vespertilionoidea, although some immunological evidence links it with the Noctilionoidea (=Phyllostomoidea). We have sequenced 402 bp of the mitochondrial cytochrome b gene for M. tuberculata (Gray in Dieffenbach, 1843), and using both our own and published DNA sequences for taxa in both superfamilies, we applied different tree reconstruction methods to find the appropriate phylogeny and different methods of estimating confidence in the parts of the tree. All methods strongly support the classification of Mystacina in the Noctilionoidea. Spectral analysis suggests that parsimony analysis may be misleading for Mystacina's precise placement within the Noctilionoidea because of its long terminal branch. Analyses not susceptible to long-branch attraction suggest that the Mystacinidae is a sister family to the Phyllostomidae. Dating the divergence times between the different taxa suggests that the extant chiropteran families radiated around and shortly after the Cretaceous-Tertiary boundary. We discuss the biogeographical implications of classifying Mystacina within the Noctilionoidea and contrast our result with those classifications placing Mystacina in the Vespertilionoidea, concluding that evidence for the latter is weak.
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Papers by Juan Morales