Papers by MINERVA RAMOS GOMEZ
Industrial Crops and Products, 2018
The objective of the study was to determine the NO-inhibitory effect of moringa infusion/aqueous ... more The objective of the study was to determine the NO-inhibitory effect of moringa infusion/aqueous preparation on a mouse macrophage RAW264 cell line. Total soluble phenols, total flavonoids, and simple phenolic contents and the antioxidant capacity of the moringa aqueous infusion and decoction were determined in order to select the best sample for NO-inhibitory assay. NO-inhibitory effect of the selected moringa infusion, was dose dependent and almost similar to the positive control (dexamethasone). A cup of the selected moringa infusion contained 70.2 mg of total soluble phenols and 30.5 mg of total flavonoids. Gallic acid and rutin were the major simple phenolics detected. The extraction efficiency of gallic acid and rutin from moringa leaves by decoction and infusion procedures were from 54.1 to 66.2% and from 39.6 to 52.4%, respectively. The order of the antioxidant capacity of moringa decoctions and infusions, from low to high, was decoction, boiling 30 min < decoction, 15 min < infusion reposed 15 min < infusion, 5 min for ABTS, decoction, 15 min < infusion, 15 min < decoction, 30 min < infusion, 5 min for DPPH, and decoction, 15 min < infusion, 15 min < decoction, 30 min = infusion, 5 min for FRAP. This is the first report concerning the content of phenolic compounds, antioxidant capacity and NO-inhibitory effect of moringa aqueous infusion that are commonly consumed around the world.
Molecules
Currently, research is focused on bioactive compounds with the potential to promote macrophage po... more Currently, research is focused on bioactive compounds with the potential to promote macrophage polarization with the aim of reducing the development of inflammatory-related diseases. However, the effect of bioactive compounds under oxidative-stress-induced hyperglycemia on macrophage polarization has been scarcely investigated. RAW 264.7 macrophages were incubated under standard (SG) or high glucose (HG) conditions and stimulated with lipopolysaccharide (LPS) (10, 60 and 100 ng/mL) to monitor macrophage polarization after resveratrol (RSV) or 3H-1,2-dithiole-3-thione (D3T) supplementation (2.5, 5, 10 and 20 µM). Under SG and HG conditions without LPS stimulation, RSV significantly decreased macrophage viability at the highest concentration (20 µM), whereas D3T had no or low effect. LPS stimulation at 60 and 100 ng/mL, under SG and HG conditions, increased significantly macrophage viability. Both RSV and D3T significantly decreased NO production in LPS-stimulated macrophages under HG...
The FASEB Journal, Apr 1, 2016
Abstract: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbal... more Abstract: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbalance in the expression of certain genes that activate or inhibit vital cellular functions of kidney. Despite several recent advances, the pathogenesis of DN remains far from clear, suggesting the need to carry out studies identifying molecular aspects, such as gene expression, that could play a key role in the development of DN. There are several techniques to analyze transcriptome in living organisms. In this study, the suppression subtractive hybridization (SSH) method was used to generate up- and down-regulated subtracted cDNA libraries in the kidney of streptozotocin (STZ)-induced diabetic rats. Northern-blot analysis was used to confirm differential expression ratios from the obtained SSH clones to identify genes related to DN. 400 unique SSH clones were randomly chosen
Journal of Food Biochemistry
Journal of Food Science and Technology, 2019
Since the fruits of Lycium L. species (Fructus lycii, goji berries) are promoted as a "superfood"... more Since the fruits of Lycium L. species (Fructus lycii, goji berries) are promoted as a "superfood" with plenty of health benefits, there is extensive research interest in their nutritional and phytochemical composition. In the present study, the nutritional value, minerals, fatty acid composition, and bioactive compounds of L. barbarum L., red, yellow, and black goji berry (L. ruthenicum Murray.) cultivated in Serbia were investigated. Antioxidant and antimicrobial properties of their methanol extracts were assessed. Red goji berry had the highest content of fats, dietary fiber, iron, total carotenoids, and 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG). The yellow goji berry extract showed the highest level of flavonoids and the most prominent antimicrobial (especially against Gram-negative bacteria) properties. The highest total phenolic content and the most potent antioxidant activity were observed for the extract of black goji berry. Therefore, all goji berries could be a valuable source of bioactive compounds in the food and pharmaceutical industry.
Food and Chemical Toxicology, 2015
Red oak (Quercus spp.) leaves are traditionally used as food in Mexico, and some of their infusio... more Red oak (Quercus spp.) leaves are traditionally used as food in Mexico, and some of their infusions have potential anticarcinogenic and anti-inflammatory effects; however, these properties have not yet been scientifically tested. The aim of this work was to explore the anti-inflammatory activity in HT-29 cells and anticarcinogenic effect in 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis of red oak infusions. Quercus infusions were prepared and administered as the sole source of drink to male Sprague-Dawley rats (1% w/v) for the entire 26-week experimental period. On week 4, rats received 8 subcutaneous injections of DMH (21 mg/kg body weight) once a week. The results showed that mean tumor (0.9 ± 0.2 vs. 2.6 ± 0.3) and multiplicity (1.2 ± 0.1 vs. 2.0 ± 0.23), and β-catenin protein level (2.2-fold) in adenocarcinomas were significantly lower in Quercus sideroxyla-treated group compared with DMH group. By contrast, Quercus durifolia and Quercus eduardii infusions had no protective effect. Additionally, the experiments in HT-29 cells confirmed that Q. sideroxyla infusion effectively decreased the levels of the inflammatory markers COX-2 and IL-8 by modulating the expression of NF-κB. These results highlight some of the molecular mechanisms related to the chemopreventive effect of Q. sideroxyla infusion and its potential value as a source of bioactive compounds.
The consumption of dry common beans (Phaseolus vulgaris) has been associated with a decrease risk... more The consumption of dry common beans (Phaseolus vulgaris) has been associated with a decrease risk for a wide variety of chronic and degenerative diseases such as cancer, obesity, diabetes and cardiovascular diseases. Beans are considered as a good source of high protein content, complex carbohydrates, dietary fiber and some vitamins and minerals. In addition to these nutritional components, common beans are rich in a variety of several phytochemicals with potential health benefits such as polyphenolic compounds, fiber, lectins and trypsin inhibitors, among others. Therefore, the scope of this chapter is to review the Correspondence/Reprint request: Dr. Loarca-Pina, G., Programa de Posgrado en Alimentos del Centro de la Republica (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Queretaro, Queretaro, Qro., 76010 México. E-mail: loarca@uaq.mx Reynoso-Camacho, R et al. 218 role of some bioactive compounds present in common beans (Phas...
Revista Mexicana De Ciencias Agricolas, Jun 1, 2012
* Recibido: agosto de 2011 Aceptado: febrero de 2012 de los flavonoides sobre esta enzima se cons... more * Recibido: agosto de 2011 Aceptado: febrero de 2012 de los flavonoides sobre esta enzima se considera uno de los mecanismos antiinf lamatorios celulares más importantes (Surh et al., 2001). Por lo tanto, es importante la identificación de fuentes con alto contenido de flavonoides que sean aceptadas por la población en general. A este respecto, el consumo de bebidas, como las infusiones, es una opción accesible para proveer de este tipo de compuestos (Høstmark, 2010). suggest that boldo and peppermint commercial infusions show biological properties with potential benefits for health.
Current Issues in Molecular Biology
Overweight and obesity have become worldwide health issues in most countries. Current strategies ... more Overweight and obesity have become worldwide health issues in most countries. Current strategies aimed to prevent or reduce overweight and obesity have mainly focused on the genes and molecular mechanisms that give the functional characteristics to different types of adipose tissue. The Browning phenomenon in adipocytes consists of phenotypic and metabolic changes within white adipose tissue (WAT) activated by thermogenic mechanisms similar to that occurring in brown adipose tissue (BAT); this phenomenon has assumed great relevance due to its therapeutic potential against overweight and obesity. In addition, the study of inflammation in the development of overweight and obesity has also been included as a relevant factor, such as the pro-inflammatory mechanisms promoted by M1-type macrophages in adipose tissue. Studies carried out in this area are mainly performed by using the 3T3-L1 pre-adipocyte cell line, testing different bioactive compound sources such as plants and foods; neve...
ANNUAL REPORT- …, 2006
123 EFFECT OF COMMON BEAN (PHASEOLUS VULGARIS) CONSUMPTION ON COLON CANCER IN SPARGUE-DAWLEY RATS... more 123 EFFECT OF COMMON BEAN (PHASEOLUS VULGARIS) CONSUMPTION ON COLON CANCER IN SPARGUE-DAWLEY RATS MC Ríos-Uglade1, R. Reynoso-Camacho1, I. Torres-Pacheco2, JA Acosta-Gallegos2, AC Palomino-Salinas2, M. Ramos-Gómez2, E. González ...
Journal of Food Science and Technology
International Journal of Molecular Sciences
The Insulin-like growth factor-I/Insulin-like growth factor-I receptor (IGF-1/IGF-1R) system is a... more The Insulin-like growth factor-I/Insulin-like growth factor-I receptor (IGF-1/IGF-1R) system is a major determinant in colorectal cancer (CRC) pathogenesis. Probiotics (Bifidobacterium longum, BF) and lycopene (LYC) have been individually researched for their beneficial effects in the prevention of CRC. However, the effect of a combined treatment of microencapsulated BF and LYC on IGF-1/IGF-1R/IGFBPs (Insulin-like growth factor-binding proteins) expression in an azoxymethane (AOM)-dextran sulfate sodium (DSS)-induced CRC model have not been demonstrated. BF was microencapsulated by the spray drying technique, with high viability, and daily gavaged with LYC for 16 weeks to CD-1 mice in an AOM-DSS model. The results indicated that BF- and BF + LYC-treated groups had significantly lower inflammation grade, tumor incidence (13–38%) and adenocarcinoma (13–14%) incidence compared to the AOM + DSS group (80%), whereas LYC treatment only protected against inflammation grade and incidence. C...
Plant Foods for Human Nutrition, 2012
Natural oleoresins rich in lycopene were obtained from two varieties of tomato (Zedona and Girond... more Natural oleoresins rich in lycopene were obtained from two varieties of tomato (Zedona and Gironda) and their nutraceutical potential (antioxidant and antimutagenic capacity) was evaluated. Both oleoresins had a high content of lycopene, 58.33±1.67 mg/g (Zedona) and 63.97±0.80 mg/g (Gironda). The antioxidant activity (AA) of the oleoresins by β-carotene method were 56.4-74.5% (Zedona) and 51-72.8% (Gironda), while when using the free radical stable 2,2-diphenyl-picryl-hydrazyl (DPPH) method, the antiradical activity (ARA) was determined to be 18.2-32.7% (Zedona) and 16.6-26.7% (Gironda) for the concentrations tested that of 200-400 μM equivalents of lycopene. The antimutagenic activity of the oleoresins was tested against aflatoxin B 1 (AFB 1) using the microsuspension assay, both varieties had a very high antimutagenic potential against AFB 1 (60-66%).These results suggest the NCRT can be taken advantage to obtaining rich oleoresin in lycopene with a nutraceutical value. Keywords Aflatoxin B 1. Antimutagenic activity. Antioxidant capacity. Lycopene. Lycopersicon esculentum. Oleoresin. Salmonella typhimurium Abbreviations NCRT Non commercial red tomato AA Antioxidant activity DPPH 2,2-diphenyl-2-picrylhydrazyl ARA Antiradical activity AFB 1 Aflatoxin B 1 BHT Butylated hydroxytoluene ADIBA α, α′-Azodiisobutyramidine dihydrochloride DMSO Dimethyl sulfoxide
Journal of Agricultural and Food Chemistry, 2008
Common beans ( Phaseolus vulgaris L.) contain a high proportion of undigested carbohydrates (NDC)... more Common beans ( Phaseolus vulgaris L.) contain a high proportion of undigested carbohydrates (NDC) that can be fermented in the large intestine to produce short-chain fatty acids (SCFA) such as acetate, propionate, and butyrate. The objective of the present study was to evaluate the composition and chemopreventive effect of a polysaccharide extract (PE) from cooked common beans ( P. vulgaris L) cv. Negro 8025 on azoxymethane (AOM) induced colon cancer in rats. The PE induced SCFA production with the highest butyrate concentrated in the cecum zone: 6.7 +/- 0.06 mmol/g of sample for PE treatment and 5.29 +/- 0.24 mmol/g of sample for PE + AOM treatment. The number of aberrant crypt foci (ACF) and the transcriptional expression of bax and caspase-3 were increased, and rb expression was decreased. The data suggest that PE decreased ACF and had an influence on the expression of genes involved in colon cancer for the action of butyrate concentration.
Agricultura técnica en …, 2007
Proceedings of the National Academy of Sciences, 2001
Induction of phase 2 enzymes, which neutralize reactive electrophiles and act as indirect antioxi... more Induction of phase 2 enzymes, which neutralize reactive electrophiles and act as indirect antioxidants, appears to be an effective means for achieving protection against a variety of carcinogens in animals and humans. Transcriptional control of the expression of these enzymes is mediated, at least in part, through the antioxidant response element (ARE) found in the regulatory regions of their genes. The transcription factor Nrf2, which binds to the ARE, appears to be essential for the induction of prototypical phase 2 enzymes such as glutathione S -transferases (GSTs) and NAD(P)H:quinone oxidoreductase (NQO1). Constitutive hepatic and gastric activities of GST and NQO1 were reduced by 50–80% in nrf2 -deficient mice compared with wild-type mice. Moreover, the 2- to 5-fold induction of these enzymes in wild-type mice by the chemoprotective agent oltipraz, which is currently in clinical trials, was almost completely abrogated in the nrf2 -deficient mice. In parallel with the enzymatic ...
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Papers by MINERVA RAMOS GOMEZ